研究目的
To investigate the photocatalytic and anticancer activity of green synthesized silver nanoparticles using leaf extract of Justicia adhatoda.
研究成果
The study successfully synthesized silver nanoparticles using Justicia adhatoda leaf extract, which exhibited significant photocatalytic and anticancer activities. The nanoparticles were characterized by various techniques, confirming their size, shape, and stability. The photocatalytic activity was size-dependent, and the nanoparticles showed potent anticancer activity against A549 cell line.
研究不足
The study focuses on the synthesis and characterization of AgNPs using Justicia adhatoda leaf extract and their applications in photocatalytic degradation and anticancer activity. However, the mechanisms behind the photocatalytic and anticancer effects are not fully explored, and the study is limited to in-vitro experiments.
1：Experimental Design and Method Selection:
The study involved the green synthesis of silver nanoparticles (AgNPs) using leaf extract of Justicia adhatoda. The synthesis was monitored by UV-Vis spectral analysis, and the nanoparticles were characterized using HRSEM, TEM, DLS, FTIR, SERS, and XRD.
2：Sample Selection and Data Sources:
Justicia adhatoda leaves were collected, dried, and ground into powder. The aqueous extract was prepared and used for the synthesis of AgNPs.
3：List of Experimental Equipment and Materials:
UV-Vis spectrophotometer (Perkin-Elmer Lamda-45), HRSEM with EDAX, TEM (Hitachi E-1010 model and TEM-JEOL model 1200EX), DLS (Horiba SZ 100), XRD (Enraf Nonius CAD-4), FTIR (Perkin-Elmer model spectrum RXI), SERS (Nano photon-Raman 11 spectrometer).
4：Experimental Procedures and Operational Workflow:
The synthesis involved mixing leaf extract with AgNO3 solution, monitoring the reaction by UV-Vis spectroscopy, and characterizing the nanoparticles using various techniques. The photocatalytic activity was tested using methylene blue dye, and anticancer activity was evaluated against A549 cell line.
5：Data Analysis Methods:
The data were analyzed using UV-Vis spectroscopy for photocatalytic activity and MTT assay for anticancer activity.
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