研究目的
Investigating the feasibility of combining optoacoustics (OA) and near-infrared optical tomography (NIROT) for spectral correction of OA signals to quantify oxygen saturation levels in tissue vasculature.
研究成果
The hybrid OA/NIROT system effectively corrected spectral distortions in OA signals, reducing errors in absorption coefficient ratios from over 100% to less than 20%. This allows for accurate estimation of oxygen saturation levels in vessels, with potential errors below 10% for arteries. The approach provides valuable complementary information for monitoring tissue perturbations and vascular oxygenation, with implications for clinical applications like neonatal brain imaging.
研究不足
The study was conducted on phantoms, not in vivo, limiting direct clinical applicability. The accuracy depends on proper identification of vessel signals and is affected by system noise, motion artifacts, and uncertainties in optical properties. The method may not perform as well in highly complex or heterogeneous real tissues.
1：Experimental Design and Method Selection:
The study used a hybrid OA/NIROT system in reflection mode. OA imaging resolved submillimetric vessel-like absorbers, while NIROT estimated light fluence for spectral correction. Theoretical models included light diffusion equations and finite element methods via NIRFAST software.
2：Sample Selection and Data Sources:
Two cuboid phantoms mimicking infant brain tissue were used. Phantom 1 had a homogeneous scattering background with three dye-filled tubes as vessels. Phantom 2 included additional absorbing cylinders to simulate perturbations.
3：List of Experimental Equipment and Materials:
Equipment included a diode-pumped Q-switched Nd:YAG laser with OPO (Spitlight DPSS OPO, InnoLas Laser GmbH), linear array ultrasound probe (ATL L7-4, Philips N.V.), research ultrasound system (V 1-64, Verasonics), NIRS device (Imagent, ISS Inc.), and materials like fat emulsion (SMOFlipid 20%, Fresenius Kabi), ICG dye (IR-125, ACROS Organics), and silicone (SILPURAN 2420 A/B, Wacker Chemie AG).
4：Experimental Procedures and Operational Workflow:
OA measurements involved laser illumination at multiple wavelengths, signal acquisition with ultrasound probe, and reconstruction using frequency-domain algorithms. NIROT measurements used fiber-based illumination and detection, with data acquisition via rotation for tomographic reconstruction. Fluence was estimated using NIRFAST for spectral correction of OA signals.
5：Data Analysis Methods:
Data analysis included segmentation of OA signals based on spectral correlation, statistical analysis of pixel values in supports, and comparison to reference values from Monte Carlo simulations or analytical models.
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Naphthol Green B
Naphthol Green B for microscopy
Sigma-Aldrich
Dye used in background medium for optical properties.
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Spitlight DPSS OPO
Spitlight DPSS OPO
InnoLas Laser GmbH
Provides laser pulses for optoacoustic imaging illumination.
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ATL L7-4
ATL L7-4
Philips N.V.
Linear array ultrasound probe for detecting optoacoustic signals.
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V 1-64
V 1-64
Verasonics
Research ultrasound system for signal acquisition and processing.
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Imagent
Imagent
ISS Inc.
Near-infrared spectroscopy device for NIROT measurements.
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SMOFlipid 20%
SMOFlipid 20%
Fresenius Kabi
Fat emulsion used as background medium in phantoms.
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IR-125
IR-125
ACROS Organics
Indocyanine Green dye for mimicking blood absorption in vessels.
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SILPURAN 2420 A/B
SILPURAN 2420 A/B
Wacker Chemie AG
Silicone material for creating absorbing cylinders in phantoms.
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ELASTOSIL RAL blue 5022
ELASTOSIL RAL blue 5022
Wacker Chemie AG
Dye for coloring silicone to create optical heterogeneities.
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Polyethylene tubes
Inner diameter 0.28 mm, wall thickness 0.16 mm
Smiths Medical
Tubes used to mimic blood vessels in phantoms.
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