研究目的
To examine biodegradation under different levels of relative air humidity and to ascertain the interactive mechanism between photodegradation and biodegradation on the decomposition of lignocellulosic materials in moist conditions with UV-A supplementation.
研究成果
Straw degradation is significantly influenced by relative air humidity and UV-A irradiation. High humidity (90% RH) promotes biodegradation primarily by filamentous fungi, while the combination of UV-A and high humidity accelerates degradation through both photodegradation and biodegradation, with photodegradation playing a central role in lignin breakdown. The findings emphasize the importance of environmental factors in straw decomposition and suggest combined mechanisms for efficient degradation.
研究不足
The study was conducted under controlled laboratory conditions, which may not fully replicate natural environmental variations. The use of specific equipment and methods might limit generalizability. Potential areas for optimization include longer experimental durations or field studies to validate findings.
1:Experimental Design and Method Selection:
The study involved straw degradation experiments under four conditions: 30% RH, 60% RH, 90% RH, and UV-A + 90% RH. Methods included mass loss measurement, chemical composition analysis, thermogravimetric analysis, Fourier transform infrared spectroscopy, BIOLOG microplate assays, and scanning electron microscopy.
2:Sample Selection and Data Sources:
Rice straw was obtained from Nanjing, China, cut or ground into pieces, and oven-dried.
3:List of Experimental Equipment and Materials:
Equipment included artificial climate box (MRC-300B, Ningbo Prandt Instrument Co., Ltd, China), UV generator (ZW-P, Yishi Co., Shanghai, China), hygrothermograph (JR593, Anymetre Instrument Co., Ltd., Guangzhou, China), elemental analyzer (Multi N/C 3100 Analyzer, Analytik-Jena Group, Jena, Germany), TGA (SII-7200, Hitachi Limited, Tokyo, Japan), FTIR (J200, Thermo Fisher Scientific, Waltham, USA), BIOLOG microplates (P-96-450R-C, Genestar Limited, Shanghai, China), SEM (EVO MA, Carl Zeiss Shanghai Co. Ltd., Shanghai, China), sputter-coater (ETD-2000, Beijing Elaborate Technology Development Ltd., Beijing, China), and software (Origin Pro Version
4:0, SPSS Version 0). Materials included saturated salt solutions (MgCl2, NaBr, KNO3), and straw samples. Experimental Procedures and Operational Workflow:
Straw was treated for 150 days for humidity conditions and 180 days for UV-A + 90% RH. Mass loss was measured by oven-drying samples. Chemical composition was analyzed using Van-Soest method and elemental analysis. TGA, FTIR, BIOLOG assays, and SEM were performed as per standard protocols.
5:Data Analysis Methods:
Data were analyzed using Origin Pro and SPSS software for statistical analysis, including calculation of mass remaining, AWCD, and Shannon-Wiener Diversity Index.
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elemental analyzer
Multi N/C 3100 Analyzer
Analytik-Jena Group
Used to quantify dissolved organic carbon and nitrogen levels in the straw samples.
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thermogravimetric analyzer
SII-7200
Hitachi Limited
Used to study the pyrolysis of straw samples.
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Fourier transform infrared spectrometer
J200
Thermo Fisher Scientific
Used to study the functional groups in straw samples.
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scanning electron microscope
EVO MA
Carl Zeiss Shanghai Co. Ltd.
Used to study the surface morphological changes in the straw samples.
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artificial climate box
MRC-300B
Ningbo Prandt Instrument Co., Ltd
Used to control ambient temperature and humidity for the straw degradation experiments.
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UV generator
ZW-P
Yishi Co.
Used to provide UV-A irradiation for the straw degradation experiment under UV-A + 90% RH condition.
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hygrothermograph
JR593
Anymetre Instrument Co., Ltd.
Used to monitor ambient temperature and relative air humidity continuously during the experiments.
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BIOLOG microplates
P-96-450R-C
Genestar Limited
Used for BIOLOG microplate assays to study the functional diversity of microbial communities.
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sputter-coater
ETD-2000
Beijing Elaborate Technology Development Ltd.
Used to gold plate specimens for SEM analysis.
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software
Origin Pro Version 9.0
OriginLab
Used for statistical analysis of data.
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software
SPSS Version 22.0
SPSS Inc.
Used for statistical analysis of data.
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