研究目的
To develop an immunochromatographic test strip with different colored lines for the test and control zones using gold nanoparticles of different shapes to prevent erroneous interpretations and improve sensitivity, using T-2 toxin as an example.
研究成果
The developed immunochromatographic test strip successfully uses gold nanoparticles of different shapes to create bicolor lines, enabling easy differentiation between test and control zones and reducing misinterpretation. It achieves high sensitivity with an instrumental detection limit of 30 pg/mL for T-2 toxin in corn extracts, and the approach is universal for other applications.
研究不足
The synthesis of gold nanoflowers requires optimization of parameters like chloroauric acid concentration and seed concentration to avoid aggregation and ensure proper shape. The method may be affected by high methanol concentrations in samples, which can destabilize antibodies. Further optimization might be needed for other antigens or sample types.
1：Experimental Design and Method Selection:
The study employed a competitive immunochromatographic assay with indirect labeling. Gold nanoparticles of different shapes (spheres and nanoflowers) were synthesized and conjugated with antibodies or streptavidin. The test strip design included specific zones for antigen-antibody interactions and color development.
2：Sample Selection and Data Sources:
Corn extracts were used as samples, spiked with known concentrations of T-2 toxin. Extracts were prepared by milling corn grains and mixing with a methanol-water solution, followed by centrifugation.
3：List of Experimental Equipment and Materials:
Chemicals included chloroauric acid, sodium citrate, antibodies, BSA, etc., from suppliers like Sigma-Aldrich. Equipment included transmission electron microscope (JEM CX-100), Zetasizer Nano for dynamic light scattering, IsoFlow dispenser, Index Cutter-1, flatbed scanner (Canon Lide 90), and software like Image Tool, Total Lab, and Origin 7.
4：Experimental Procedures and Operational Workflow:
5.
4. Experimental Procedures and Operational Workflow: Synthesis of gold nanospheres and nanoflowers, conjugation with antibodies or streptavidin, preparation of test strips with applied reagents, immersion of strips in sample dilutions, incubation, scanning, and image analysis. Statistical analysis used a four-parameter sigmoid function.
5：Data Analysis Methods:
Data were analyzed using Origin 7.5 software with a sigmoid function to determine detection limits and working ranges. Intensity of coloration was measured digitally.
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