1：Experimental Design and Method Selection:

The study involved designing a hybrid carrier system by integrating DNA origami nanostructures into layer-by-layer (LbL) biopolymer microparticles. The rationale was to protect DNA nanostructures from degradation and improve their delivery efficiency. Methods included DNA origami assembly, LbL coating, stability testing using FRET, and cell interaction assays.

2：Sample Selection and Data Sources:

Silica microparticles (2.76 μm diameter) were used as templates. DNA origami nanostructures (hollow tubes) were synthesized. Human blood-derived polymorphonuclear neutrophil granulocytes (PMNs) and HEK293T/17 cells were used for enzymatic and cell interaction studies.

3：76 μm diameter) were used as templates. DNA origami nanostructures (hollow tubes) were synthesized. Human blood-derived polymorphonuclear neutrophil granulocytes (PMNs) and HEK293T/17 cells were used for enzymatic and cell interaction studies. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Equipment included flow cytometer (BD FACSCalibur), confocal laser scanning microscope (Zeiss LSM 510 META), zeta potential analyzer (Brookhaven ZetaPALS), SEM (Zeiss Gemini 500), and microplate reader (TECAN infinite M200). Materials included poly-l-arginine, dextran sulfate, silica particles, DNA staples, fluorescent dyes (Cy5, Alexa488, Alexa546), BSA, lipids (POPS, POPC), and various buffers.

4：0). Materials included poly-l-arginine, dextran sulfate, silica particles, DNA staples, fluorescent dyes (Cy5, Alexa488, Alexa546), BSA, lipids (POPS, POPC), and various buffers. Experimental Procedures and Operational Workflow:

4. Experimental Procedures and Operational Workflow: DNA origami was assembled and purified. LbL microparticles were coated with biopolymers and DNA origami layers. Stability was tested at different pH and with lysosomal enzymes. Cell uptake, viability, and lipid bilayer coating were assessed. Cargo loading with BSA was demonstrated.

5：Data Analysis Methods:

Data were analyzed using flow cytometry for fluorescence intensity and FRET efficiency, CLSM for imaging, zeta potential measurements, SEM for morphology, and statistical tests (student's t-test) for significance.