研究目的
To develop an artificial skin model using digital light processing 3D printing with silk fibroin-incorporated polyethylene glycol hydrogels for cell embedment and keratin layer formation.
研究成果
SF-PEG4A hydrogels fabricated by DLP 3D printing support high cell viability, improved mechanical properties, and effective keratin layer formation, making them suitable for artificial skin models with potential applications in pharmaceuticals and cosmetics.
研究不足
The study may have limitations in scalability for large tissue constructs, long-term stability of hydrogels, and potential variability in cell responses. Optimization of printing parameters and further in vivo validation could be areas for improvement.
1:Experimental Design and Method Selection:
The study used digital light processing (DLP) 3D printing with visible light to fabricate hydrogels from SF-PEG4A precursor solutions, incorporating cells for tissue engineering applications.
2:Sample Selection and Data Sources:
Silk fibroin particles were prepared from Bombyx mori silk cocoons; PEG4A was synthesized; fibroblast and keratinocyte cells were cultured.
3:List of Experimental Equipment and Materials:
Equipment includes a digital rheometer (MARS III, Thermo Scientific), universal testing machine (LRX Plus, Lloyd Instruments), scanning electron microscope (SUPRA SSVP, Carl Zeiss), confocal laser scanning microscope (Carl Zeiss), microplate reader (Synergy HT, BioTek), projector (Vivitek), and software (Rhinoceros
4:0, XMaker). Materials include PEG-hydroxyl (JenKem Technology), acryloyl chloride (Sigma Aldrich), triethylamine (Sigma Aldrich), N-vinylpyrrolidone (Sigma Aldrich), eosin Y disodium (Sigma Aldrich), triethanolamine (Sigma Aldrich), DMEM (Invitrogen), fetal bovine serum (Life Technologies), penicillin/streptomycin (Life Technologies), LIVE/DEAD viability/cytotoxicity kit (Invitrogen), alamarBlue (Invitrogen), rhodamine-labeled phalloidin (Life Technologies), DAPI (Sigma Aldrich), and others. Experimental Procedures and Operational Workflow:
Steps include preparation of PEG4A and SF particles, mixing precursor solutions, 3D printing with DLP, cell culture, viability and proliferation assays (live/dead, alamarBlue), cell adhesion staining (phalloidin/DAPI), and epidermal differentiation at air-liquid interface with H&E staining.
5:Data Analysis Methods:
Rheological and mechanical properties were analyzed; cell viability and proliferation were assessed using fluorescence and absorbance measurements; statistical analysis was performed.
独家科研数据包,助您复现前沿成果,加速创新突破
获取完整内容-
digital rheometer
MARS III
Thermo Scientific
Analyzing rheological behaviors of hydrogels
-
scanning electron microscope
SUPRA SSVP
Carl Zeiss
Obtaining morphological images of hydrogels
-
universal testing machine
LRX Plus
Lloyd Instruments
Determining compressive strengths of hydrogels
-
confocal laser scanning microscope
Carl Zeiss
Collecting fluorescence images for cell viability assays
-
microplate reader
Synergy HT
BioTek
Assaying fluorescence for cell proliferation
-
projector
Vivitek
Projecting light patterns for DLP 3D printing
-
software
Rhinoceros 5.0
Robert McNeel & Associates
Designing patterns for 3D printing
-
software
XMaker
Yinzhou District, Ningbo, Zhejiang, China
Slicing layers for 3D printing
-
4-arm PEG-hydroxyl
20 kDa
JenKem Technology
Precursor for PEG4A synthesis
-
acryloyl chloride
Sigma Aldrich
Reactant for PEG4A synthesis
-
triethylamine
Sigma Aldrich
Reactant for PEG4A synthesis
-
N-vinylpyrrolidone
Sigma Aldrich
Component in precursor solution
-
eosin Y disodium
Sigma Aldrich
Photoinitiator in precursor solution
-
triethanolamine
Sigma Aldrich
Component in precursor solution
-
DMEM
Invitrogen
Cell culture medium
-
fetal bovine serum
Life Technologies
Supplement for cell culture
-
penicillin/streptomycin
Life Technologies
Antibiotic for cell culture
-
LIVE/DEAD viability/cytotoxicity kit
Invitrogen
Assessing cell viability
-
alamarBlue
Invitrogen
Assessing cell proliferation
-
rhodamine-labeled phalloidin
Alexa Fluor 594
Life Technologies
Staining F-actin for cell adhesion
-
登录查看剩余18件设备及参数对照表
查看全部