研究目的
To develop a theranostic nanoplatform for on-demand CO release combined with chemotherapy and photothermal therapy for enhanced cancer treatment.
研究成果
The MCM@PEG-CO-DOX NPs enable on-demand release of CO and DOX via NIR irradiation, enhancing chemotherapy efficacy through mitochondrial targeting and ROS generation, with effective dual-mode imaging and significant tumor growth inhibition in vivo, demonstrating potential for biomedical applications.
研究不足
The study is limited to in vitro and in vivo models using specific cell lines and mice; clinical applicability and long-term effects are not addressed. The photothermal effect may not be sufficient for complete tumor ablation without combination therapy.
1：Experimental Design and Method Selection:
The study involved synthesizing magnetic carbon nanoparticles (MagC NPs), coating them with MIL-100 MOF, functionalizing with PEG and DABPY for Mn carbonyl chelation, loading with doxorubicin (DOX), and evaluating for CO release, photothermal effects, drug delivery, cytotoxicity, and in vivo antitumor efficacy using NIR irradiation.
2：Sample Selection and Data Sources:
HCT116 human colon cancer cells and nude mice with HCT116 tumors were used. Materials were sourced from Sigma-Aldrich, Seebio Biotech, KeyGen BioTech, Beyotime Biotechnology, and Gibco.
3：List of Experimental Equipment and Materials:
Equipment included TEM, DLS, ICP-OES, GC-MS, UV-Vis spectrophotometer, infrared thermal imaging camera, flow cytometer, confocal microscope, MRI and PAI systems. Materials included ferrocene, FeCl3·6H2O, H3BTC, Mn(CO)5Br, DOX, and various chemicals.
4：Experimental Procedures and Operational Workflow:
Synthesis steps involved solvothermal reactions, coating, functionalization, drug loading, and in vitro/in vivo assays for release, imaging, and therapy with NIR irradiation control.
5：Data Analysis Methods:
Data were analyzed using GraphPad Prism for statistical significance, with methods like ICP-OES for Mn quantification, GC-MS for CO release, and flow cytometry for cell uptake and apoptosis.
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Optima 8000 ICP-OES
8000
PerkinElmer
Used for measuring the concentration of Mn in samples to calculate CO immobilization.
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Infrared thermal imaging camera
VarioCAM?hr research
InfraTec
Used for recording temperature changes during photothermal experiments.
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GC-MS
Used for analyzing the amount of released CO from nanoparticles.
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808 nm laser
Changchun New Industries Optoelectronics Technology
Used for NIR irradiation to trigger photothermal effects and release of CO and DOX.
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Flow cytometer
BD FACSAria I
BD
Used for analyzing cell uptake and apoptosis rates.
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Confocal laser microscope
Carl Zeiss
Used for colocalization analysis and imaging of cells.
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ELISA reader
SynergyTM
BioTeK
Used for measuring cell viability in cytotoxicity assays.
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MRI imaging system
3.0-T
Used for in vivo magnetic resonance imaging of tumors.
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PAI imaging system
Used for in vivo photoacoustic imaging of tumors.
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