研究目的
To develop a highly efficient but simple single-component organic photothermal therapy system in the biological transparent window based on biomimetic self-assembling phthalocyanines for cancer treatment.
研究成果
Bio-ZnPc-Pdots exhibit high photothermal conversion efficiency (38.2%) at 808 nm, effective in vitro and in vivo cancer cell eradication through PTT without significant ROS production or systemic toxicity, and show potential for PA imaging-guided therapy, demonstrating a biomimetic approach for clinical PTT applications.
研究不足
The study focuses on specific cell lines and mouse models; generalizability to other cancers or in vivo conditions may require further investigation. The use of intratumoral injection limits direct applicability to systemic administration. Long-term toxicity and biodegradability aspects are not fully explored.
1：Experimental Design and Method Selection:
Inspired by natural bacteriochlorophylls, biomimetic self-assembling tetrahexanoyl phthalocyanine (Bio-ZnPc) was synthesized and characterized. Amphiphilic polymer (H-PEG-PPG-PEG-OH) was used to make it water-dispersible. Photothermal performance, stability, and photochemistry were studied using UV-vis absorption, fluorescence, DLS, TEM, and other spectroscopic methods. In vitro and in vivo PTT efficacy was evaluated using cell cultures and tumor-bearing mice.
2：Sample Selection and Data Sources:
Liver hepatocellular carcinoma cells (HepG2) and murine tumor cell line 4T1 were used. Samples included Bio-ZnPc, H2Pc, ZnPc, and their polymer dots (Pdots). Data were obtained from laboratory experiments.
3：List of Experimental Equipment and Materials:
Phthalocyanines (Bio-ZnPc, H2Pc, ZnPc), amphiphilic polymer H-PEG-PPG-PEG-OH, solvents (chloroform-D, methanol-D4, THF, PBS), laser (808 nm), infrared thermal camera, DLS instrument, TEM, UV-vis spectrophotometer, fluorescence spectrometer, MTT assay kit, calcein-AM, propidium iodide, ABMDMA for singlet oxygen detection.
4：Experimental Procedures and Operational Workflow:
Synthesis of Bio-ZnPc and controls; preparation of Pdots via polymer encapsulation; characterization using NMR, UV-vis, fluorescence, DLS, TEM; photothermal efficiency measurements under laser irradiation; stability tests; in vitro cytotoxicity and PTT assays on HepG2 cells; in vivo PTT and PA imaging on 4T1 tumor-bearing mice; histological analysis with H&E staining.
5：Data Analysis Methods:
Photothermal conversion efficiency calculated using a reported method; statistical analysis of cell viability and tumor volume changes; PA signal intensity quantification.
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Laser
808 nm
Used for photothermal therapy and irradiation in experiments
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Infrared Thermal Camera
Monitoring temperature changes during photothermal therapy
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Dynamic Light Scattering Instrument
Measuring particle size distribution of Pdots
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Transmission Electron Microscope
Imaging morphology of Pdots
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UV-vis Spectrophotometer
Recording absorption spectra
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Fluorescence Spectrometer
Recording fluorescence emission spectra
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MTT Assay Kit
Assessing cell viability
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Photoacoustic Imaging System
Performing in vitro and in vivo PA imaging
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