研究目的
To develop a novel fluorometric method for detecting glucose based on the in-situ formation of silicon nanodots from a redox reaction between glucose and aminopropyltriethoxysilane, aiming for high sensitivity, specificity, and applicability in biological samples without using enzymes.
研究成果
The developed method successfully enables fluorometric detection of glucose through in-situ synthesis of silicon nanodots, offering high sensitivity (detection limit of 7 μM), wide linear range (10 μM to 0.9 mM), good specificity (minimal interference from amino acids), and applicability in complex biological samples like human serum with satisfactory recoveries. It represents a novel, enzyme-free approach with potential for further optimization and application in bioimaging and sensing.
研究不足
The method has cross-reactivity with other reductive saccharides like galactose and mannose, requires a heating process and rather long assay time (90 minutes), and may not be suitable for rapid point-of-care testing due to these factors.
1：Experimental Design and Method Selection:
The method involves a one-step synthesis of silicon nanodots (SNDs) through a redox reaction between glucose and aminopropyltriethoxysilane (APTES) under mild conditions (50°C for 90 minutes). Fluorescence spectroscopy is used for detection based on the turn-on signal from SND formation.
2：Sample Selection and Data Sources:
Glucose solutions of varying concentrations (0 to 900 μM) and spiked human serum samples were used. Samples were prepared by mixing APTES and glucose, followed by heating.
3：List of Experimental Equipment and Materials:
Transmission electron microscopy (TEM) for morphology, dynamic light scattering (DLS) for size distribution, Fourier transform infrared (FT-IR) spectroscopy for chemical bonds, X-ray photoelectron spectroscopy (XPS) for surface analysis, UV-Vis spectrometer (Agilent Cary model) for absorption, fluorescence spectrophotometer (Edinburgh Instrument FSL980 for quantum yield, F-7000 Fluorescence Spectrometer for photostability), and standard laboratory equipment for heating and mixing.
4：Experimental Procedures and Operational Workflow:
Mix APTES and glucose in aqueous solution, heat at 50°C for 90 minutes to form SNDs. Characterize SNDs using TEM, DLS, FT-IR, XPS, UV-Vis, and fluorescence spectroscopy. Perform fluorescence measurements at excitation 410 nm and emission 475 nm for glucose detection. Test selectivity with amino acids and other saccharides. Apply to serum samples with spiked glucose for recovery tests.
5：Data Analysis Methods:
Fluorescence intensity data were analyzed to establish calibration curves (linear ranges 10-100 μM and 150-900 μM). Detection limit calculated as 3 times signal-to-noise ratio. Statistical analysis for recoveries and RSD in serum samples.
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