研究目的
To develop a synthesis method for silica-coated quantum dot-densely-embedded silica nanoparticles with remarkably brighter fluorescence for improved sensitivity in biological detection applications.
研究成果
The Si@D-QD@Si NPs were successfully synthesized with significantly higher fluorescence intensity due to increased QD embedding, making them suitable for sensitive detection in biological fields. Future work could focus on enhancing quantum yield further and testing in vivo applications.
研究不足
The study may have limitations in scalability, potential aggregation issues, and the need for further optimization for specific biological applications. The quantum yield improvement was slight, and the method's applicability to other types of QDs or environments was not fully explored.
1：Experimental Design and Method Selection:
The study involved synthesizing silica nanoparticles using the St?ber method, modifying them with thiol groups, embedding quantum dots (QDs) densely onto the surface, and coating with a silica shell to enhance fluorescence brightness and biocompatibility.
2：Sample Selection and Data Sources:
Silica nanoparticles of about 150 nm diameter were used as templates. Red, green, and blue QDs were purchased from Zeus.
3：List of Experimental Equipment and Materials:
Materials included tetraethylorthosilicate (TEOS), 3-mercaptopropyl trimethoxysilane (MPTS), dichloromethane, ethyl alcohol, aqueous ammonium hydroxide, and QDs. Equipment included transmission electron microscopes (LIBRA 120, JEM-2010), UV/Vis spectrophotometer (Mecasys OPTIZEN POP), fluorometer (Model Cary Eclipse), inductively coupled plasma spectrometer (720 ICP-OES), quantum yield measurement system (QE-2000), confocal micro-Raman system (LabRam 300), optical microscope (BX41), and nanoparticle tracking analysis (NTA).
4：Experimental Procedures and Operational Workflow:
Steps included preparation of thiol-modified silica NPs, embedding QDs by stirring in dichloromethane and ethanol mixtures, adding MPTS and NH4OH for silica coating, centrifugation, washing, and characterization using TEM, UV-Vis, fluorescence spectroscopy, ICP, QY measurement, and single-particle fluorescence intensity measurement.
5：Data Analysis Methods:
Data were analyzed for size distribution, absorbance, fluorescence intensity, quantum yield, and cadmium content using statistical methods and software tools inherent to the equipment.
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Optical Microscope
BX41
Olympus
Equipped with the micro-Raman system for fluorescence signal collection.
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Objective Lens
×100 0.90 NA
Olympus
Used for collecting fluorescence signals in the micro-Raman system.
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Nanoparticle Tracking Analysis
NTA
Malvern
Used to measure the overall size of nanoparticles.
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Transmission Electron Microscope
LIBRA 120
Carl Zeiss
Used to obtain TEM images of nanoparticles for characterization.
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Transmission Electron Microscope
JEM-2010
JEOL
Used to obtain TEM images of nanoparticles for characterization.
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Fluorometer
Cary Eclipse
Agilent Technologies
Used to obtain fluorescence emission spectra of nanoparticles.
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Inductively Coupled Plasma Spectrometer
720 ICP-OES
Agilent Technologies
Used for ICP analysis to measure cadmium content in nanoparticles.
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Confocal Micro-Raman System
LabRam 300
JY-Horiba
Used to measure fluorescence intensity of single nanoparticles.
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Tetraethylorthosilicate
Sigma Aldrich
Used as a precursor for synthesizing silica nanoparticles.
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3-mercaptopropyl trimethoxysilane
Sigma Aldrich
Used for thiol modification of silica nanoparticles to facilitate QD embedding.
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Dichloromethane
Samchun
Used as a solvent in the QD embedding process.
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Ethyl alcohol
99.9%
Daejung
Used as a solvent and for washing nanoparticles.
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Aqueous ammonium hydroxide
27%
Daejung
Used as a catalyst in the silica synthesis and coating processes.
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Quantum dots
Zeus
Embedded onto silica nanoparticles to enhance fluorescence.
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UV/Vis Spectrophotometer
OPTIZEN POP
Mecasys
Used to measure extinction properties and absorbance of samples.
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Quantum Yield Measurement System
QE-2000
Otsuka Electronics
Used to measure the quantum yield of nanoparticles.
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