研究目的
To design and synthesize a near-infrared fluorescence chemosensor based on isothiocyanate-aza-BODIPY for sensitive and selective detection of cyanide ions at low concentrations in buffer media and living cells.
研究成果
The aza-BODIPY-based sensor N1 is highly sensitive and selective for cyanide detection with a low detection limit of 19 ppb in buffer media, suitable for environmental and biological applications. It demonstrates effective use in living cell imaging with low cytotoxicity, offering dual optical responses for visual and instrumental detection.
研究不足
The sensor operates optimally in 50% PBS buffer/acetonitrile mixtures, which may limit its use in purely aqueous environments. Detection is specific to cyanide, but interference from high concentrations of other nucleophiles was not fully explored. The synthesis yield is moderate (50%), and cytotoxicity increases at higher concentrations (e.g., 70% viability at 50 μM).
1：Experimental Design and Method Selection:
The study involved designing and synthesizing a chemosensor (N1) using aza-BODIPY with isothiocyanate groups for cyanide sensing via nucleophilic addition. Methods included synthesis, characterization (NMR, mass spectrometry), UV/Vis and fluorescence spectroscopy, cyclic voltammetry, and confocal microscopy for cell imaging.
2：Sample Selection and Data Sources:
Cyanide ions (from potassium salts) and other anions were used as analytes. 4T1 breast cancer cells were used for biological testing.
3：List of Experimental Equipment and Materials:
Instruments included Bruker Avance 300 NMR spectrometer, Hewlett Packard 8453 spectrophotometer, Perkin Elmer LS 55B luminescence spectrometer, ThermoElectron LCQ-DECA-XP mass spectrometer, Autolab potentiostat PGSTAT 12 for CV, and Nikon A1 confocal microscope. Materials included reagents from Sigma-Aldrich and Fluka.
4：Experimental Procedures and Operational Workflow:
Synthesis of N1 via condensation reaction; sensitivity and selectivity tests in buffer solutions; binding studies using Benesi-Hildebrand and Job's plot methods; cellular imaging with confocal microscopy; cytotoxicity assay using MTT method.
5：Data Analysis Methods:
Data analyzed using fluorescence titration for detection limit and association constant, NMR for binding mechanism, and DFT calculations for molecular orbitals.
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