研究目的
To construct luminescent lanthanide-collagen peptide hybrid three-dimensional nanofibrous scaffolds for pH-controlled drug delivery, mimicking native collagen structures and enabling tunable photoluminescence and pH-responsiveness.
研究成果
The constructed luminescent collagen peptide-lanthanide scaffolds successfully mimic native collagen structures, exhibit pH-responsive self-assembly and drug delivery capabilities, and are biocompatible. They offer a novel approach for pH-controlled drug delivery with potential applications in regenerative medicine and cancer therapy.
研究不足
The study is limited to in vitro experiments; in vivo efficacy and long-term stability were not assessed. The pH-responsiveness may vary with different drugs or biological environments, and scalability for clinical applications needs further investigation.
1：Experimental Design and Method Selection:
The study involved designing collagen mimetic peptides with specific terminal amino acids to bind lanthanide ions, triggering self-assembly into nanofibrous scaffolds. Methods included peptide synthesis, circular dichroism spectroscopy, scanning electron microscopy, fluorescence spectroscopy, turbidity measurements, drug loading and release experiments, and cytotoxicity assays.
2：Sample Selection and Data Sources:
Three peptides (DDColDD, DWColWD, HWColWD) were synthesized and characterized. Lanthanide ions (e.g., La3+, Eu3+, Tb3+) and model drugs (camptothecin, cefoperazone sodium) were used. Data were collected from spectroscopic and microscopic analyses.
3：List of Experimental Equipment and Materials:
Peptides synthesized using Fmoc solid phase synthesis, lanthanide ions, buffers (e.g., HEPES, MES, acetic acid/sodium acetate), drugs, and cells (HELA cells). Equipment included Chirascan CD spectrometer, Hitachi S-4800 SEM, Hitachi FLS920 spectrofluorometer, JASCO V-630 spectrophotometer, and ELx800 Absorbance Microplate Reader.
4：Experimental Procedures and Operational Workflow:
Peptides were synthesized, purified, and characterized. Assembly was triggered by adding lanthanide ions at specific pH conditions. Morphology was analyzed via SEM, photoluminescence via fluorescence spectroscopy, turbidity via spectrophotometry, drug loading and release via UV measurements, and cytotoxicity via MTT assay.
5：Data Analysis Methods:
Data were analyzed using standard curves for UV measurements, thermal unfolding curves for CD, and statistical analysis for cytotoxicity.
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