1：Experimental Design and Method Selection:

The study involved fabricating PMMA-rGO composite films using solvent evaporation technique and exposing them to gamma radiation at doses of 25 kGy, 50 kGy, and 100 kGy to investigate changes in structural, morphological, and biological properties. Theoretical models included XRD for phase analysis, FTIR for functional groups, and various spectroscopic and microscopic techniques for characterization.

2：Sample Selection and Data Sources:

Samples included pristine PMMA-rGO films and irradiated variants. Materials were sourced from commercial suppliers (e.g., Alfa Aesar for PMMA, Merck India for chemicals). Data were collected through laboratory experiments.

3：List of Experimental Equipment and Materials:

Equipment included PANalytical X'Pert Powder XRD System, FTIR-6300 spectrometer, Carl Zeiss MA 15/EVO 18 SEM, KRUSS DSA-10 contact angle system, Jasco UV-Vis-NIR spectrometer, Dimension Edge-Bruker AFM, and gamma chamber (1200 Gamma Chamber from Inter University Accelerator Center). Materials included PMMA, graphite flakes, acids, and other chemicals.

4：Experimental Procedures and Operational Workflow:

Films were prepared by dissolving PMMA in acetone, adding rGO, evaporating solvent, and irradiating with gamma source. Characterization involved XRD, FTIR, SEM, contact angle measurements, DRS, AFM, TGA, hemocompatibility tests, antimicrobial assays, and cell culture studies (MTT assay and DAPI staining).

5：Data Analysis Methods:

Data were analyzed using standard techniques: XRD for phase identification, FTIR for functional groups, SEM and AFM for morphology, contact angle for wettability, DRS for optical properties, TGA for thermal stability, and statistical methods for biological assays (e.g., percentage calculations for cell viability).