研究目的
To develop a highly selective fiber optic surface plasmon resonance-based dopamine sensor using a molecular imprinted GNP/SnO2 nanocomposite for efficient detection of dopamine, addressing the limitations of existing methods such as complexity, cost, and lack of specificity.
研究成果
The developed SPR-based fiber optic sensor using molecular imprinted GNP/SnO2 nanocomposite is highly sensitive, selective, and stable for dopamine detection, with a low LOD of 0.031 μM. It offers advantages of simplicity, low cost, fast response, and suitability for remote sensing, making it promising for practical applications in health monitoring.
研究不足
The sensor's calibration curve saturates at higher dopamine concentrations due to finite binding sites. The aggregation of GNP at high concentrations can reduce effectiveness. The study does not address potential issues in real-world applications such as sample matrix effects or long-term stability in biological fluids.
1：Experimental Design and Method Selection:
The study uses surface plasmon resonance (SPR) technique with a fiber optic sensor. The sensing layer is a molecular imprinted polymer (MIP) made from graphene nanoplatelets (GNP) and tin oxide (SnO2) nanoparticles nanocomposite. The methodology involves synthesis of the nanocomposite, optimization of fabrication parameters, and characterization using wavelength interrogation.
2：Sample Selection and Data Sources:
Dopamine samples are prepared in phosphate buffer saline (PBS) with concentrations ranging from 0.05 μM to 100 μM. Interferands such as ascorbic acid, urea, and creatinine are also used for selectivity tests.
3：05 μM to 100 μM. Interferands such as ascorbic acid, urea, and creatinine are also used for selectivity tests. List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Equipment includes a tungsten halogen lamp, microscope objective, spectrometer, flow cell, three-dimensional stage, scanning electron microscope (SEM), sonicator, thermal evaporation system. Materials include optical fiber, silver, GNP, SnO2 nanoparticles, dopamine, methacrylic acid (MAA), ethylene glycol dimethacrylate (EGDMA), azobisisobutyronitrile (AIBN), acetonitrile, dimethylformamide (DMF), ethylene glycol, methanol, PBS buffer.
4：Experimental Procedures and Operational Workflow:
The probe is fabricated by leaching cladding from a fiber, coating with silver, and then coating with the MIP layer. The MIP is synthesized by preparing GNP/SnO2 nanocomposite, mixing with dopamine and other reagents, polymerizing, and removing the template. Optimization involves varying dipping time, GS concentration, DA concentration, and template removal time. SPR spectra are recorded for different DA concentrations to analyze shifts in resonance wavelength.
5：Data Analysis Methods:
Data is analyzed by measuring shifts in resonance wavelength from SPR spectra. Sensitivity is calculated as the derivative of the calibration curve. Limit of detection (LOD) is evaluated using spectrometer resolution and sensitivity.
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tungsten halogen lamp
Light source for the experimental setup to provide broadband light for SPR measurements.
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microscope objective
Used to couple light into the fiber probe in the experimental setup.
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spectrometer
Interfaced with a computer to record the spectrum of output light from the probe for SPR analysis.
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flow cell
Holds the analyte sample and the probe during experiments.
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three-dimensional stage
Used to position the flow cell for optimal light coupling into the fiber probe.
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scanning electron microscope
SEM
Used for morphological characterization of the sensing layer to confirm the formation of imprints.
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sonicator
Used for mixing and dispersing materials during the synthesis of the nanocomposite and MIP.
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thermal evaporation system
Used to coat a 40 nm thick silver layer over the unclad core of the optical fiber.
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