1：Experimental Design and Method Selection:

The study designed a nanoconstruct using gold nanorods coated with mesoporous silica, pegylated, modified with d-CPP, and loaded with Ce6 for combined PTT and PDT. Methods included synthesis, characterization, cellular uptake studies, cytotoxicity assays, apoptosis mechanism analysis, and in vivo efficacy testing in nude mice.

2：Sample Selection and Data Sources:

Human breast cancer MCF-7 cells were used, obtained from National Infrastructure of Cell Line Resources. In vivo studies used female Nu/Nu nude mice with MCF-7 xenografts.

3：List of Experimental Equipment and Materials:

Equipment included UV-Vis-NIR spectrophotometer (UV-1800PC, Mapada Instruments), Malvern Zetasizer, transmission electron microscope (JEM1400PLUS, JEOL), fluorescence microscope (IX-81, Olympus), fluorescence spectrophotometer (Cary Eclipse, Agilent), multimode reader (FlexStation 3, Molecular Devices), Operetta high-content screening system (PerkinElmer). Materials included chloroauric acid, CTAB, NaBH4, AgNO3, AA, TEOS, APTES, maleimide-PEG2000-NHS, mPEG2000-NHS, d-CPP, RPMI-1640 medium, FBS, and various chemicals from specified suppliers.

4：Experimental Procedures and Operational Workflow:

Synthesis of AuNRs by seed-mediated growth, coating with SiO2, pegylation, d-CPP modification, Ce6 loading. Characterization included extinction spectra, zeta potential, size, entrapment efficiency. Cellular uptake studies with inhibitors, cytotoxicity assays under dark, PTT, PDT, and combined conditions, apoptosis mechanism studies, and in vivo treatment with laser irradiation.

5：Data Analysis Methods:

Statistical analysis using one-way ANOVA with Bonferroni correction for multiple comparisons. Data presented as mean ± SD.