研究目的
To investigate the connection between electrical signal-induced systemic photosynthetic response and changes in the photochemical reflectance index (PRI) in pea plants, and to assess the potential of PRI for monitoring this response.
研究成果
Electrical signals induce systemic photosynthetic responses in pea leaves, including decreases in quantum yields of PSI and PSII and increases in nonphotochemical quenching, which are strongly correlated with changes in PRI. The changes in PRI are primarily due to energy-dependent quenching. PRI shows potential for monitoring systemic photosynthetic responses linked to long-distance electrical signalling, with optimal measurement using unmodified PRI and 10-nm spectral bands.
研究不足
The study is limited to pea plants under controlled laboratory conditions; applicability to other species or field conditions is not verified. The PRI changes were relatively small, and noise in measurements could affect accuracy. The method may not capture all aspects of photosynthetic responses, and further optimization is needed for broader use.
1：Experimental Design and Method Selection:
The study used local burning of a leaflet to induce electrical signals (variation potentials) in pea seedlings, with measurements of electrical activity, photosynthetic parameters (quantum yields of PSI and PSII, nonphotochemical quenching), and PRI in intact leaves. The design aimed to correlate PRI changes with photosynthetic responses induced by electrical signals.
2：Sample Selection and Data Sources:
Pea (Pisum sativum L.) seedlings, 14–21 days old, were cultivated hydroponically under controlled conditions (24°C, 16:8-h light:dark photoperiod) in a plant growth chamber. Each experiment used a separate seedling, with multiple repetitions as specified in figures.
3：List of Experimental Equipment and Materials:
Equipment included extracellular Ag+–AgCl electrodes (EVL-1M3.1), a high-impedance amplifier (IPL-113), a Dual-PAM-100 fluorometer, a halogen lamp (Osram Decostar), a spectrometer (S100), and a grey card (QPcard 101 Calibration Card ver. 3). Materials included pea seedlings, electroconductive gel (Uniagel), and hydroponic setup.
4：1), a high-impedance amplifier (IPL-113), a Dual-PAM-100 fluorometer, a halogen lamp (Osram Decostar), a spectrometer (S100), and a grey card (QPcard 101 Calibration Card ver. 3). Materials included pea seedlings, electroconductive gel (Uniagel), and hydroponic setup.
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Seedlings were adapted for 75 min before stimulation (15 min dark, 60 min under actinic light). Electrical signals were induced by local burning, and measurements were taken in second and fourth leaves. Photosynthetic parameters were measured using saturation pulses, and PRI was calculated from reflectance spectra. Data were averaged and analyzed statistically.
5：Data Analysis Methods:
Data were analyzed using Student's t-test for significant differences, linear regression for correlations, and determination coefficients. Spectral data were processed with specific bandwidths for PRI calculation.
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