1：Experimental Design and Method Selection:

The study used liquid crystalline networks (LCN) prepared by photopolymerization of acrylate monomers with different alignments (planar homogeneous, homeotropic, isotropic) to serve as cell scaffolds. The rationale was to test if molecular alignment in the polymer could induce cellular alignment in myoblast cultures.

2：Sample Selection and Data Sources:

C2C12 murine myoblasts were used as the cell line, selected for their relevance to skeletal muscle models. The LCN films were fabricated with varying compositions (e.g., 20% or 40% mol mol?1 crosslinker) and alignments.

3：List of Experimental Equipment and Materials:

Materials included commercial monomers (e.g., Synthon Chemical mesogens), photoinitiator (Irgacure 369 from Sigma-Aldrich), liquid crystalline cells with polyimide or PVA coatings, UV lamp (Thorlabs M385L2-C4), SEM (Zeiss Sigma), AFM (Park System XE-7), tensiometer (Krüss K100), and biological assay reagents (e.g., Diff-Quik Kit).

4：Experimental Procedures and Operational Workflow:

Monomeric mixtures were melted, infiltrated into LC cells, aligned, polymerized with UV light, sterilized, and cut into stripes. Myoblasts were seeded on the films, incubated for 72 hours, stained, and analyzed using optical microscopy. Statistical analysis of cell alignment was performed using MATLAB scripts.

5：Data Analysis Methods:

Cell alignment was quantified using order parameter S calculated from nucleus orientation angles, with statistical analysis over ensembles of cells. Surface morphology was assessed via SEM and AFM, and wettability was measured via contact angles.