研究目的
To develop a freezing-induced turn-on strategy for selective real-time imaging of frozen cancer cells during cryosurgery to improve therapeutic efficacy by enabling accurate determination of frozen tissue volume and differentiation from normal cells.
研究成果
The synthesis of TABD-Py enables a unique freezing-induced turn-on imaging modality for cryosurgery, with high specificity to cancer cells, biocompatibility, and real-time monitoring capability. This approach enhances therapeutic efficacy by allowing precise imaging of frozen tissues, reducing damage to normal cells, and holds promise for clinical applications in cancer treatment.
研究不足
The study is limited to in vitro experiments with specific cell lines; in vivo applications and clinical translation require further investigation. The mechanism of ice interaction and specificity to cancer cells may need more detailed molecular studies. Potential optimizations include improving dye stability and expanding to other cancer types.
1：Experimental Design and Method Selection:
The study synthesized an AIE fluorogen (TABD-Py) and investigated its interaction with ice crystals. Methods included fluorescence spectroscopy, microscopy, and cell culture experiments to assess AIE characteristics, ice crystal growth, and cell-specific imaging.
2：Sample Selection and Data Sources:
Samples included TABD-Py solutions in various solvents, pure water, and cell lines (HeLa, NIH 3T3, MSC, HUVEC, MCF-7, HepG2). Data were obtained from controlled freezing experiments and confocal laser scanning microscopy.
3：2). Data were obtained from controlled freezing experiments and confocal laser scanning microscopy.
List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Equipment included fluorescence microscopes, confocal laser scanning microscope, UV light source (365 nm), and materials like TABD-Py, commercial dyes (Neutral Red, LyroTracker, MitoTracker), and cell culture reagents.
4：Experimental Procedures and Operational Workflow:
Procedures involved preparing TABD-Py solutions, measuring emission spectra in solvent mixtures, observing ice crystal growth, staining cells with dyes, freezing samples, and imaging with CLSM at room temperature and frozen states.
5：Data Analysis Methods:
Data analysis included comparing PL intensities, calculating enhancement factors, assessing cytotoxicity via inhibitory concentration, and evaluating selectivity through retention ratios and imaging contrast.
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