1：Experimental Design and Method Selection:

The study designed a one-pot, one-step reaction in aqueous solution using organosilane precursors, salts, and surfactants or amphiphilic polymers to synthesize organosilica nanoparticles under ambient conditions without additional solvents, energy, or harsh chemicals. The rationale was to mimic biomimetic synthesis for eco-friendly production.

2：Sample Selection and Data Sources:

Samples were prepared using various organosilanes (e.g., MPTMS, MPMDMS, VTMS, MTMS), salts (e.g., sodium nitrite, fluoride, dibasic phosphate, acetate, sulfite), surfactants (e.g., Tween series, SDS, CTAB), and polymers (e.g., PVA, PVP). Deionized water was used as the solvent. Selection criteria included common availability and amphiphilic properties.

3：List of Experimental Equipment and Materials:

Instruments included a microplate spectrophotometer (Thermo Fisher Scientific Multiskan GO) for turbidity measurements, dynamic light scattering instruments (Horiba LB-500 and Malvern Zetasizer Nano Series) for size and zeta potential, SEM (JSM-7600F field emission scanning electron microscope, JEOL) for imaging, TEM (JEOL JEM-2000EXII) for internal structure, solid-state NMR (Bruker Avance III spectrometer) for silicon structure, ATR-FTIR (IRAffinity-1S, Shimadzu with MIRacle ATR unit, PIKE Technologies) for in-situ monitoring, optical microscope (CKX53, Olympus with DP22 CCD camera) for real-time imaging, and elemental analyzer (VARIO EL cube, Elementar GmbH). Materials included organosilanes, salts, surfactants, polymers, and Ellman reagent (DTNB) from suppliers like Sigma-Aldrich and J.T.Baker.

4：Experimental Procedures and Operational Workflow:

Reactions were set up by mixing organosilane, surfactant, and salt in deionized water with gentle pipetting, then incubated at ambient temperature without stirring. High-throughput screening used 96-well microplates with real-time turbidity monitoring at 800 nm. Samples were purified by centrifugation and washing for characterization. Kinetic studies measured remaining silane using DTNB assay. In-situ ATR-FTIR and microscopic imaging were performed at specified intervals.

5：Data Analysis Methods:

Data were analyzed using statistical techniques for mean values and standard deviations (n=3 for replicates). Software tools included those integrated with the instruments (e.g., for NMR, FTIR, DLS). Turbidity kinetics, size distributions, zeta potentials, and elemental compositions were quantified and compared.