研究目的
To synthesize novel cyanoarylporphyrazines with triazole groups and study their potential as photosensitizers for photodynamic therapy and optical probes for intracellular viscosity.
研究成果
The novel cyanoarylporphyrazines with triazole groups exhibit promising photodynamic activity and sensitivity to intracellular viscosity, making them potential candidates for photodynamic therapy and as optical probes. Their reduced dark cytotoxicity is advantageous for therapeutic applications.
研究不足
The study is limited to in vitro experiments with specific cell lines; in vivo applications and broader biological contexts were not explored. The sensitivity to viscosity and photodynamic effects may vary in different environments.
1：Experimental Design and Method Selection:
The study involved the synthesis of cyanoarylporphyrazines using click chemistry and template self-assembly methods, followed by characterization of their photophysical properties, cytotoxicity, and accumulation in cancer cells.
2：Sample Selection and Data Sources:
A431 human skin epidermoid carcinoma cells were used for in vitro studies.
3：List of Experimental Equipment and Materials:
Instruments included IR spectrometer (FSM 1201), UV-Vis spectrometer (PerkinElmer Lambda 25), NMR spectrometer (Bruker Avance II+), fluorescence spectrometer (PerkinElmer LS 55), mass spectrometer (Bruker Microflex LT MALDI TOF), viscometer (Anton Paar SVM 3000), plate spectrophotometer (BioTek Synergy MX), and chemicals from Sigma Aldrich.
4：Experimental Procedures and Operational Workflow:
Synthesis of compounds, measurement of absorption and fluorescence spectra, viscosity studies, cell culture incubation, MTT assay for cytotoxicity, and fluorescence microscopy for accumulation studies.
5：Data Analysis Methods:
Data were analyzed using F?rster-Hoffman equations for viscosity effects and statistical methods for cytotoxicity assessments.
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UV-Vis spectrometer
Lambda 25
PerkinElmer
Recording electronic absorption spectra
-
NMR spectrometer
Avance II+
Bruker
Recording 1H and 13C NMR spectra
-
Fluorescence spectrometer
LS 55
PerkinElmer
Studying steady-state fluorescence
-
Mass spectrometer
Microflex LT
Bruker
Obtaining MALDI TOF mass spectra
-
Viscometer
SVM 3000 Stabinger
Anton Paar
Measuring dynamic viscosity
-
IR spectrometer
FSM 1201
Not specified
Recording IR spectra of compounds
-
Plate spectrophotometer
Synergy MX
BioTek
Reading absorbance in MTT assay
-
MTT reagent
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
Alfa Aesar
Used in cytotoxicity assay
-
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