研究目的
To develop a molecular hybrid capable of simultaneously generating singlet oxygen and nitric oxide upon single photon excitation with green light for multimodal anticancer and antibacterial treatments.
研究成果
The molecular hybrid 1 effectively generates both singlet oxygen and nitric oxide upon green light excitation, with high quantum yields and oxygen independence for NO release. It shows good biocompatibility in the dark and enhanced photomortality in cancer cells due to bimodal action, making it a promising candidate for advanced phototherapeutic applications.
研究不足
The quantum yield for NO photodecomposition is relatively low (ΦP = 0.002), and the study is preliminary, requiring further mechanistic investigations and in vivo validation. The efficiency under different biological conditions and scalability for clinical use are not fully addressed.
1：Experimental Design and Method Selection:
The study involved designing a molecular hybrid (compound 1) by nitrosation of a precursor (compound 2), with the BODIPY unit serving as a light-harvesting antenna. Theoretical models included molecular dynamics simulations and DFT calculations to understand molecular interactions.
2：Sample Selection and Data Sources:
Solutions of compounds 1 and 2 in methanol and water-methanol mixtures were used, with concentrations around
3：5 μM. Biological tests used human fibroblasts, melanoma A375, and hepatocellular carcinoma HepG2 cell lines. List of Experimental Equipment and Materials:
Equipment included a green light source (532 nm), spectrophotometer for absorption measurements, fluorimeter for fluorescence quantum yield, HPLC for analysis, amperometric NO electrode for NO detection, and MTT assay kit for cytotoxicity. Materials included methanol, water, and the synthesized compounds.
4：Experimental Procedures and Operational Workflow:
Solutions were irradiated with green light; 1O2 was detected via phosphorescence at 1270 nm, NO release was monitored amperometrically, absorption spectral changes were tracked over time, and cell viability was assessed after incubation and irradiation.
5：Data Analysis Methods:
Data were analyzed using standard photochemical equations (e.g., Rehm-Weller for electron transfer), quantum yields were calculated, and statistical analysis of biological assays was performed.
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