研究目的
Designing a method to detect Ag+ with high selectivity, sensitivity, and efficiency in water samples due to environmental pollution concerns.
研究成果
The QCy probe is highly sensitive and selective for Ag+ detection with a low detection limit of 0.03 μM, suitable for environmental monitoring. It shows practical applicability in real water samples with good recovery rates, making it a reliable and economical tool for Ag+ sensing.
研究不足
The probe may have slightly lower recovery rates in river water due to complex oxides affecting fluorescence response. pH sensitivity limits optimal performance to pH 5-8, with best results at pH 7.0. Specificity to Ag+ over other ions like Hg2+ may require further validation in complex matrices.
1：Experimental Design and Method Selection:
Designed and synthesized a heptamethine cyanine-based fluorescent probe QCy for Ag+ detection based on intramolecular d–π interactions. Used fluorescence and UV-vis spectroscopy for characterization and titration experiments.
2：Sample Selection and Data Sources:
Prepared simulated wastewater and collected real water samples (tap water, Xuanwu Lake, Qinhuai River). Cationic salts and reagents were obtained from commercial suppliers.
3：List of Experimental Equipment and Materials:
Instruments included pH meter, NMR spectrometer, mass spectrometer, UV-vis spectrometer, fluorescence spectrometer. Materials included solvents, cationic salts, and synthesized compounds.
4：Experimental Procedures and Operational Workflow:
Synthesized QCy through multi-step organic synthesis. Conducted fluorescence titration with Ag+ in EtOH/PBS buffer, measured selectivity against other metal ions, time dependence, pH titration, and binding mechanism studies. Applied probe to water samples for recovery tests.
5：Data Analysis Methods:
Used linear regression for detection limit calculation (3σ/k method), Job's plot for stoichiometry, Benesi-Hildebrand equation for association constant, and statistical analysis for recovery rates.
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