研究目的
To develop a rapid, sensitive, and multiplex fluorescent biosensor for the detection of pathogenic DNAs using ultrathin metal-organic framework nanosheets.
研究成果
The ultrathin MOF nanosheets were successfully prepared and used to develop a sensitive and selective fluorescent biosensor for multiplex detection of pathogenic DNAs with low detection limits, paving the way for rapid and robust biosensing applications.
研究不足
The paper does not explicitly mention limitations, but potential areas could include the specificity in complex samples, scalability for industrial applications, and optimization for other pathogens or conditions.
1：Experimental Design and Method Selection:
A surfactant-assisted one-step method was used to prepare ultrathin Cu-TCPP MOF nanosheets, which exhibit high affinity for ssDNA and fluorescence quenching properties. The biosensor operates on the principle of fluorescence restoration upon target DNA hybridization.
2：Sample Selection and Data Sources:
DNA sequences for Salmonella enterica, Listeria monocytogenes, and Vibrio parahemolyticus genes were synthesized and used as probes and targets.
3：List of Experimental Equipment and Materials:
Chemicals included copper nitrate trihydrate, PVP, TCPP, DMF, ethanol, trifluoroacetic acid, and DNA sequences. Equipment included TEM (JEOL-JEM-1200EX), SEM (Hitachi-SU8010), AFM (Dimension Icon, Bruker), and a fluorescence spectrometer (BioTek ELIASA Synergy Neo2).
4：2). Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: MOF nanosheets were synthesized by mixing precursors, heating, and centrifuging. DNA detection involved hybridizing probes with targets, adding MOF nanosheets, incubating, and measuring fluorescence.
5：Data Analysis Methods:
Fluorescence spectra were recorded, and detection limits were calculated using the formula LOD = 3σ/S, with linear ranges determined from calibration plots.
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Transmission Electron Microscope
JEM-1200EX
JEOL
Characterization of Cu-TCPP nanosheets
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Scanning Electron Microscope
SU8010
Hitachi
Characterization of Cu-TCPP nanosheets
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Atomic Force Microscope
Dimension Icon
Bruker
Characterization of Cu-TCPP nanosheets in tapping mode
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Polyvinylpyrrolidone
Average wt 40,000
Sigma-Aldrich
Surfactant used in synthesis of MOF nanosheets
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Fluorescence Spectrometer
Synergy Neo2
BioTek
Recording fluorescence spectra for DNA detection
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Copper Nitrate Trihydrate
Sigma-Aldrich
Chemical used in synthesis of MOF nanosheets
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Tetrakis(4-carboxyphenyl)porphyrin
97%
Tokyo Chemical Industry
Organic ligand used in synthesis of MOF nanosheets
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N,N-Dimethylformamide
99.8%
Alfa Aesar
Solvent used in synthesis
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Trifluoroacetic Acid
99%
Alfa Aesar
Acid used in synthesis
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DNA Sequences
Sangon Biotech
Probes and targets for DNA detection
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