1：Experimental Design and Method Selection:

The study involved synthesizing a fluorescent probe (DCPO-DMTC) based on oxidative deprotection of dimethylthiocarbamate (DMTC) groups by hypochlorite, leading to a turn-on NIR fluorescence response. UV-Vis and fluorescence spectroscopy were used to monitor the response, and confocal microscopy for cell imaging.

2：Sample Selection and Data Sources:

Hypochlorite and other reactive oxygen species (ROS) were generated from commercial sources or chemical reactions. RAW 264.7 murine macrophage cells were used for bioimaging studies.

3：7 murine macrophage cells were used for bioimaging studies. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Instruments included Bruker AV-400 NMR spectrometer, Thermo Q Exactive mass spectrometer, UV-Vis spectrophotometer, Hitachi F-7000 fluorescence spectrophotometer, Thermo Electron EVOS fluorescence microscope, and Zeiss LSM710META confocal microscope. Materials were obtained from J&K China Chemical Ltd and General-Reagents, including solvents, silica gel for chromatography, and chemicals for ROS generation.

4：Experimental Procedures and Operational Workflow:

The probe was synthesized and characterized. UV-Vis and fluorescence spectra were recorded in buffer solutions with varying hypochlorite concentrations. Selectivity was tested against other ROS. Cytotoxicity was assessed via MTT assay. Cell culture and imaging were performed with stimulation by LPS and PMA to induce hypochlorite production.

5：Data Analysis Methods:

Fluorescence intensity was measured and analyzed for linearity and limit of detection (LOD). Statistical analysis included standard deviation calculations for LOD. ImageJ software was used for semi-quantitative analysis of fluorescence images.