1：Experimental Design and Method Selection:

The study used a photo-electro-oxidation (PEO) process with UV light and electrical current to generate hydroxyl radicals for viral degradation. The experimental design involved treating a solution containing HAdV-5 with PEO for 3 hours at a 5A current, with samples collected every 15 minutes for analysis.

2：Sample Selection and Data Sources:

A solution was prepared from sterile water containing

3：107 genomic copies/L of HAdV-5, divided into test and control parts. The virus was cultured in A-549 cells. List of Experimental Equipment and Materials:

Equipment included a benchtop reactor with a borosilicate glass kettle, Ti/TiO2 cathode, Ti/70TiO2-30RuO2 anode, 400W mercury-vapor lamp, electrical current source, centrifugal pump, chemical thermometer, pH indicator strips, real-time thermocycler (iQ5 Bio-Rad), and various kits and reagents (e.g., Invitek DNA/RNA Virus Mini Kit, Platinum SYBR Green qPCR Supermix-UDG, TURBO DNase). Materials included sterile water, HAdV-5, anhydrous sodium sulfate, and cell culture components.

4：Experimental Procedures and Operational Workflow:

The test solution was treated in the PEO reactor; aliquots were collected at intervals and stored at -70°C. pH and temperature were monitored. Viral genome extraction was performed using commercial kits, followed by qPCR with specific primers. DNAse treatment was applied to assess viral integrity, and ICC-qPCR was used for infectivity analysis.

5：Data Analysis Methods:

qPCR results were analyzed using iQ5 software, with standard curves for quantification. Log10 reduction in viral load was calculated to assess efficiency, following U.S. EPA and Health Canada guidelines for a 4 log10 reduction as effective.