1：Experimental Design and Method Selection:

The study involved synthesizing HA-ZnPP conjugates to enhance water solubility and tumor targeting via the EPR effect. Methods included chemical synthesis, physicochemical characterization, in vitro cellular uptake studies, and in vivo antitumor efficacy assessments in mouse models.

2：Sample Selection and Data Sources:

HeLa cells and S-180 mouse sarcoma cells were used for in vitro and in vivo experiments, respectively. Materials were sourced from commercial suppliers.

3：List of Experimental Equipment and Materials:

Equipment included high-performance size exclusion chromatography (HPSEC), dynamic light scattering (DLS) instrument, electron spin resonance (ESR) spectrometer, fluorescence spectrophotometer, and xenon lamp for light irradiation. Materials included ZnPP, HA, solvents, and reagents from Sigma-Aldrich, Wako Pure Chemical, and others.

4：Experimental Procedures and Operational Workflow:

Synthesis of ZnPPED and conjugation with HA, purification, characterization of particle size and singlet oxygen generation, cellular uptake assays in HeLa cells, body distribution studies in tumor-bearing mice, and antitumor activity assessments with light irradiation.

5：Data Analysis Methods:

Data were analyzed using fluorescence spectrometry for drug quantification, ESR for singlet oxygen detection, and statistical analysis with Student's t-test.