研究目的
To evaluate the biodegradability of disulfide-organosilica nanoparticles for potential use in cancer therapy by examining their reactions with glutathione using various spectroscopic and microscopic techniques.
研究成果
MPDMS nanoparticles contain disulfide bonds and are biodegradable in response to glutathione, making them promising for cancer therapy applications. The use of soft X-ray photoelectron spectroscopy provided novel insights into surface chemistry, demonstrating the reduction of disulfide bonds and oxidation of GSH to GSSG. This biodegradability feature enhances their potential as safe and effective drug delivery vehicles.
研究不足
Radiation damage from X-rays can affect organic components, requiring careful optimization of irradiation time. XPS may not detect disulfide bonds deep inside nanoparticles due to surface sensitivity. The degradation process is slow, taking up to 7 days, which might not be ideal for all clinical applications. Sample preparation and handling could introduce contaminants or oxidation.
1：Experimental Design and Method Selection:
The study involved synthesizing two types of thiol-organosilica nanoparticles (MPMS NPs and MPDMS NPs) via sol-gel chemistry, incubating them with glutathione (GSH) to assess biodegradability, and analyzing the products using FE-SEM, Raman spectroscopy, and soft X-ray photoelectron spectroscopy (XPS) to investigate chemical and morphological changes.
2：Sample Selection and Data Sources:
Nanoparticles were synthesized from precursors MPMS and MPDMS. GSH aqueous solution (10 mM) was used for incubation. Samples were prepared on Au/Cr/Si substrates for analysis.
3：List of Experimental Equipment and Materials:
FE-SEM instrument (S4800, Hitachi, Japan), Raman spectrophotometer (NRS3100, JASCO, Japan), XPS setup at BL23SU in SPring-8, centrifuge, incubator, rotation mixer, Milli-Q water, GSH, MPMS, MPDMS, NH4OH.
4：Experimental Procedures and Operational Workflow:
NPs were synthesized, washed, incubated with GSH at 37°C for 7 days, washed again, and then analyzed. FE-SEM images were taken at 5 kV acceleration voltage. Raman spectra were measured with a 532 nm laser. XPS measurements used synchrotron radiation at 1150 eV photon energy with short irradiation times to minimize damage.
5：Data Analysis Methods:
Raman spectra were deconvoluted using pseudo-Voigt functions. XPS spectra were analyzed using CasaXPS software for peak decomposition. FE-SEM images were used to observe morphological changes.
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