研究目的
To investigate the environmentally friendly method for testing photocatalytic inactivation of cyanobacterial propagation on a hybrid Ag-TiO2 photocatalyst under solar illumination.
研究成果
The hybrid Ag-TiO2/DM photocatalyst effectively inactivates cyanobacteria under solar illumination, with significant damage to cell walls and photosynthetic activity. However, it increases EPS concentration and has a slight effect on DBP formation, suggesting the need for further research on mutagenic potential and application in water treatment.
研究不足
The study is limited to specific cyanobacterial species (Microcystis aeruginosa and Oscillatoria tenuisa) and conditions (e.g., solar lamp simulation, specific dosages). Potential optimizations include testing under natural sunlight, varying environmental factors, and scaling up for real-world applications.
1:Experimental Design and Method Selection:
The study used a hybrid Ag-TiO2/DM photocatalyst synthesized by the sol-gel method, with photocatalytic reactions performed in a semi-batch reactor under solar lamp illumination. Endpoints included chlorophyll a, photosynthetic efficiency, and flow cytometry measurements.
2:Sample Selection and Data Sources:
Axenic cultures of Microcystis aeruginosa and Oscillatoria tenuisa cyanobacteria were obtained from Renyi-Tan Reservoir, Taiwan, and grown in standard media.
3:List of Experimental Equipment and Materials:
Equipment included a solar lamp (Suntest AM1, Hanau, Germany), flow cytometer (FACScan, D-48161 Partec, Münster, Germany), liquid scintillation counter (B2810TR, PerkinElmer), ICP-OES (model 2100DV, Perkin-Elmer), GC/MS (model GC-6890N, MS-5973N, Agilent), and GC/ECD (HP 6890N, Agilent). Materials included titanium isopropoxide, isopropyl alcohol, silver nitrate, diatomite, propidium iodide, SYBR green I, and various chemicals for media and analysis.
4:Experimental Procedures and Operational Workflow:
Photocatalytic reactions were conducted with varying Ag-TiO2/DM dosages (520-2100 mg/L) for up to 24 h under light. Samples were collected at intervals for analysis of chlorophyll a, photosynthetic activity (using carbon-14 method), flow cytometry, DOC, potassium ions, DBP formation, and mutagenicity tests.
5:Data Analysis Methods:
Data were analyzed using statistical methods for dose-response relationships, with specific techniques like spectrophotometry for chlorophyll a, flow cytometry for cell viability, and standard methods for DBP and mutagenicity analysis.
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Liquid Scintillation Counter
B2810TR
PerkinElmer
Measurement of radioactivity for carbon-14 assimilation in photosynthetic efficiency tests.
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ICP-OES
2100DV
Perkin-Elmer
Measurement of potassium ion concentrations.
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GC/MS
GC-6890N, MS-5973N
Agilent
Analysis of trihalomethanes (THMs) using purge-and-trap method.
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GC/ECD
HP 6890N
Agilent
Quantification of haloacetic acids (HAAs).
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Solar Lamp
Suntest AM1
Hanau
Illumination source for photocatalytic reactions, simulating solar spectrum.
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Flow Cytometer
FACScan
Partec
Analysis of cyanobacterial cells for chlorophyll-a fluorescence and cell viability.
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Dissecting Phase Contrast Microscope
SSM-422L
Microtech
Separation and observation of cyanobacterial samples.
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Sep-Pak Plus C18 Cartridges
Waters Chromatography; Millipore
Concentration of cyanobacterial suspensions for mutagenicity tests.
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