研究目的
To evaluate the feasibility of near infrared spectroscopy combined with chemometric approaches for the botanical origin prediction of honey samples.
研究成果
Near infrared spectroscopy combined with multivariate and machine learning analyses can authenticate the botanical origin of honey, particularly for acacia and polyfloral types. However, accurate classification of linden honey was not achieved, likely due to sample size and contamination issues. The methods show promise for routine online control of honey botanical origin.
研究不足
The study had limitations in classifying linden honey due to small sample size (only 7 samples) and potential contamination with other pollens. The PLS-DA model showed poor performance for chestnut and linden honeys, and SVM and CDA approaches also struggled with linden classification. The imbalance in group sizes may have affected algorithm discrimination capabilities.
1:Experimental Design and Method Selection:
The study used near infrared spectroscopy with chemometric approaches including partial least squares discriminant analysis (PLS-DA), support vector machine (SVM), and canonical discriminant analysis (CDA) for classification. Spectra pretreatments such as autoscale, standard normal variate (SNV), detrending, first derivative, and smoothing were applied to reduce scattering.
2:Sample Selection and Data Sources:
119 honey samples were collected, including 65 polyfloral, 29 acacia, 18 chestnut, and 7 linden honeys from various sources including retail and local beekeepers in Veneto, Italy.
3:List of Experimental Equipment and Materials:
A FOSS DS-2500 scanning monochromator for NIR analysis, equipped with a slurry cup with quartz window and 0.5 mm optical path gold reflector. Physical-chemical parameters were measured using instruments like Abbe Refractometer, Five Go F3, Basic 20, Jasco UV/Vis spectrophotometer, Kit PHADEBAS Honey Diastase Test, and UHPLC Nexera x2 with RID-20A detector.
4:5 mm optical path gold reflector. Physical-chemical parameters were measured using instruments like Abbe Refractometer, Five Go F3, Basic 20, Jasco UV/Vis spectrophotometer, Kit PHADEBAS Honey Diastase Test, and UHPLC Nexera x2 with RID-20A detector. Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Honey samples were homogenized, scanned in triplicate in transfectance mode over 850-2500 nm, and spectra were averaged. Absorbance data were stored as log(1/R). Wet chemistry analyses were conducted for quality assessment.
5:Data Analysis Methods:
Statistical analysis included ANOVA, PLS-DA with cross-validation, SVM with cross-validation, and CDA with feature selection using Boruta algorithm. Performance metrics such as sensitivity, specificity, accuracy, precision, and Matthews correlation coefficient were calculated.
独家科研数据包,助您复现前沿成果,加速创新突破
获取完整内容-
UHPLC system
Nexera x2 Quaternary System
Shimadzu
Used for glucose and fructose quantification in honey samples.
-
refractive index detector
RID-20A
Shimadzu
Used with UHPLC for detecting sugars in honey samples.
-
scanning monochromator
DS-2500
FOSS
Used for near infrared spectroscopy analysis of honey samples in transfectance mode.
-
Abbe Refractometer
nD 13,000–17,000 Brix 1.0–95.0%
Bormac
Used to measure moisture content in honey samples.
-
pH meter
Basic 20
Crison Instrument
Used to measure pH and free acidity of honey samples.
-
conductivity meter
Five Go F3
Mettler Toledo
Used to measure electric conductivity of honey samples.
-
UV/Vis spectrophotometer
Jasco
Used for diastase index determination in honey samples.
-
diastase test kit
PHADEBAS Honey Diastase Test
Magle
Used to determine diastase index in honey samples.
-
software
WinISI 4
FOSS Analytical A/S
Used to store and process absorbance data from NIR spectroscopy.
-
software
Matlab R2017a
MathWorks Inc.
Used for chemometric analysis including PLS-DA.
-
software
PLS Toolbox
Eigenvector Research Inc.
Used for PLS-DA modeling in chemometric analysis.
-
software
scikit-learn
Used for support vector machine (SVM) classification in Python.
-
software
SAS
SAS Institute Inc.
Used for statistical analysis including ANOVA and CDA.
-
software
XLSTAT
Addinsoft
Used for plotting CDA results.
-
software
Boruta package
Comprehensive R Archive Network
Used for feature selection in machine learning.
-
登录查看剩余13件设备及参数对照表
查看全部