研究目的
To develop a hybrid nanomaterial for synergistic tumor therapy by combining photothermal therapy and photodynamic therapy effects using a single laser source, overcoming limitations such as non-specificity and hypoxic tumor microenvironment.
研究成果
The MCSCe nanoplatform demonstrated excellent tumor accumulation, biocompatibility, and synergistic therapeutic effects through NIR-induced hyperthermia and hydroxyl radical generation, providing a promising approach for precise and effective phototherapy with minimal damage to normal tissues.
研究不足
The study may have limitations in scalability for clinical applications, potential off-target effects not fully characterized, and the need for further optimization in material synthesis and dosing. The in vivo models used are specific to mice, which may not fully translate to humans.
1：Experimental Design and Method Selection:
The study designed and synthesized a biomimetic nanoplatform (MCSCe) by dotting carbon spheres with cerium oxide and coating with cancer cell membrane. Methods included synthesis, characterization, in vitro and in vivo evaluations.
2：Sample Selection and Data Sources:
Used murine mammary carcinoma (4T1) cells, normal cells (COS7, 293T), and BALB/c mice for experiments. Data from instruments like TEM, UV-vis, ESR, etc.
3：List of Experimental Equipment and Materials:
Included resorcinol, formaldehyde, cerium nitrate, lasers, thermal imagers, spectrometers, microscopes, etc.
4：Experimental Procedures and Operational Workflow:
Steps involved synthesis of CSCe and MCSCe, photothermal evaluation, SOD-like activity assays, ROS detection, cytotoxicity tests, cellular uptake studies, mitochondrial potential changes, biodistribution, pharmacokinetics, in vivo therapy, and toxicity assessments.
5：Data Analysis Methods:
Used statistical analyses (Student's t-test), software for flow cytometry (Flow Jo), and various spectroscopic and imaging techniques for data collection and interpretation.
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ESR spectrometer
X-band spectrometer A200
Bruker
Collect Electron Spin Resonance spectra
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ICP-OES
700
Agilent
Measure cerium concentrations in tissue and tumors
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Fluorescence microscope
IX73
OLYMPUS
Visualize cell live/dead staining
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Nano-ZS ZEN3600
ZEN3600
Malvern Instruments
Measure surface zeta-potential and particle size using dynamic light scattering
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X-ray diffractometer
MiniFlex 600
Rigaku
Conduct X-ray diffraction analysis
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UV-vis spectrophotometer
Lambda Bio40
Perkin-Elmer
Collect UV-vis spectra
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Scanning electron microscope
Sigma
Sigma
Observe morphology of samples
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Transmission electron microscope
JEM-2100
JEM
Observe morphology and high-resolution details of samples
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NIR light source
808 nm
STONE Laser
Supply 808 nm near infrared light for irradiation
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Thermal imager camera
FLIR Systems AB
Monitor heat generated by light
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Microplate reader
Model 550
Bio-Rad
Evaluate cytotoxicity via optical density measurements
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Confocal laser scanning microscopy
Nikon
Obtain fluorescence microscopy images
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Flow cytometer
BD FACSAria TM III
BD
Quantitative flow cytometry measurements
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Avanti mini extruder
Avanti
Coextrude solutions through porous membranes for nanomaterial preparation
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CRi maestro small animal imaging system
maestro
CRi
Collect fluorescence signals for in vivo optical imaging
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