1：Experimental Design and Method Selection:

Ultrasound-assisted coprecipitation method followed by steam sterilization was used to synthesize SH-Eu:nHAP nanoparticles, leveraging ultrasonic sonochemistry and heparin stabilization.

2：Sample Selection and Data Sources:

Analytically pure raw materials including Ca(NO3)2·4H2O, Eu(NO3)3·6H2O, (NH4)2HPO4, and sodium heparin were used. Samples were characterized at different synthesis stages.

3：List of Experimental Equipment and Materials:

High intensity ultrasound probe, autoclave for steam sterilization, XRD (D/Max-IIIA, Rigaku Co.), FT-IR (Nexus, Thermo Nicolet), ICP-AES (Optima 4300DV, PE Co. Ltd.), HRTEM (JEM-2100F, JEOL), particle size and zeta potential analyzer (Zetasizer 3000HS, Malvern), fluorescence spectrophotometer (970CRT, Shanghai Sanco), microplate reader (Thermo Labsystems), CLSM (AOBS SP2, Leica).

4：Experimental Procedures and Operational Workflow:

Solutions of Ca2+/Eu3+ and PO4^3- were mixed, pH adjusted to 10, precipitated, centrifuged, washed, redispersed with SH, ultrasonicated for 6 min, and steam sterilized at 121°C for 1 h. Biological evaluations included hemolysis tests with rabbit RBCs, cytotoxicity with L-02 cells using MTT assay, and cell labeling with HepG2 cells observed via CLSM.

5：Data Analysis Methods:

XRD for phase composition and crystallinity, FT-IR for functional groups, ICP-AES for elemental content, TEM/HRTEM for morphology, particle size analyzer for size distribution, fluorescence spectrophotometer for luminescence spectra, statistical analysis for biological assays.