研究目的
To develop a surface plasmon resonance (SPR) based sensor chip for specific detection of blood glucose with improved sensitivity and stability, utilizing spectral interrogation and self-assembled monolayers for covalent bonding.
研究成果
The SPR-based glucose sensor demonstrates enhanced sensitivity (0.14 nm/(mg/dl)) and stability for up to 3 months, with reliable performance in real blood serum samples. The use of covalent bonding via SAMs improves reusability and reduces non-specific interactions, making it suitable for continuous monitoring applications.
研究不足
The detection limit is approximately 7 mg/dl with a standard spectrometer, which may not be sufficient for all clinical applications; potential interference from other blood analytes not fully tested; reliance on covalent bonding may have limitations in certain environments.
1：Experimental Design and Method Selection:
The experiment used a Kretschmann configuration for SPR with spectral interrogation. Self-assembled monolayers (SAMs) were employed for covalent bonding of glucose oxidase (GOx) to enhance stability and reusability.
2：Sample Selection and Data Sources:
Glucose solutions in water with concentrations from 0 to 200 mg/dl, and human and foetal bovine serums (HS and FBS) obtained commercially. Control experiments without GOx were conducted.
3：List of Experimental Equipment and Materials:
SPR setup with prism, silver-coated SF11 glass slides, GOx, SAMs materials, spectrometer for spectral measurement, and a conventional glucometer for comparison.
4：Experimental Procedures and Operational Workflow:
Immobilize GOx on sensor chips using SAMs; record SPR spectra for varying glucose concentrations and blood serums; perform control experiments without GOx; compare results with glucometer measurements.
5：Data Analysis Methods:
Analyze SPR spectra to determine resonance wavelength shifts; calculate sensitivity from response curves; use statistical comparison with control experiments and commercial device.
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