1：Experimental Design and Method Selection:

The study used UV-Vis spectroscopy, fluorescence spectroscopy (G4-FID assay), and circular dichroism (CD) to investigate interactions between phthalocyanines and various DNA structures (G-Quadruplexes and duplex DNA). Titrations were performed to assess affinity and selectivity.

2：Sample Selection and Data Sources:

DNA oligonucleotides included human telomeric repeat (AG3(T2AG3)3), bimolecular Oxytricha repeat (G4T4G4)2, tetramolecular sequence (T2G5T)4, and duplex DNA (5GC and salmon sperm DNA). Phthalocyanines (ZnPc1, ZnPc2, ZnPc3, ZnPc4) and TMPyP were synthesized or purchased.

3：List of Experimental Equipment and Materials:

Equipment included UV-2501-PC spectrophotometer (Shimadzu), Fluoromax-3 spectrofluorometer (Horiba), J-1500 and J-815 CD spectrophotometers (Jasco), confocal microscope LSM 710 (Carl Zeiss). Materials included PBS buffer, DMSO, oligonucleotides from Thermo Fisher Scientific, TMPyP from Sigma Aldrich.

4：Experimental Procedures and Operational Workflow:

Titrations involved adding DNA to phthalocyanine solutions, monitoring spectral changes. Fluorescence assays used thiazole orange displacement. CD melting experiments determined stabilization effects. Cell studies included MTT assays and fluorescence microscopy on UM-UC-3 bladder cancer cells.

5：Data Analysis Methods:

Data analyzed using Hypspec program for KD values, Hill saturation binding model for fluorescence titrations, sigmoidal curve fitting for CD melting, and non-linear regression for MTT assays.