1：Experimental Design and Method Selection:

The probe ZOC was designed based on FRET and ICT platforms using coumarin as donor and pyridinium as acceptor. The synthesis involved multi-step organic reactions including condensation and cyclization. Fluorescence and UV-Vis spectroscopy were used for characterization and detection.

2：Sample Selection and Data Sources:

Probe ZOC was synthesized and characterized. Hypochlorite solutions and other analytes (ROS, RNS, ions) were prepared from commercial chemicals. RAW 264.7 cells were used for bioimaging.

3：7 cells were used for bioimaging. List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Instruments included Bruker Avance 300 MHz NMR spectrometer, Agilent Q-TOF6510 mass spectrometer, Hitachi U-4100 UV-Vis spectrophotometer, VERTEX 70 FT-IR spectrophotometer, XD-4 melting point apparatus, PHS-3C pH meter, Pekin-Elmer LS-55 fluorescence spectrometer, and LSM 700 confocal microscope. Materials included silica gel, solvents (DCM, ethanol, CH3CN, etc.), and chemicals for analyte preparation.

4：Experimental Procedures and Operational Workflow:

Synthesis of ZOC involved stepwise reactions monitored by TLC and purified by column chromatography. Spectroscopic measurements were conducted in PBS-ethanol buffer. Cell imaging involved culturing RAW 264.7 cells, incubation with probe and stimulants, and confocal microscopy.

5：7 cells, incubation with probe and stimulants, and confocal microscopy. Data Analysis Methods:

5. Data Analysis Methods: Fluorescence ratios (I478/I610) were calculated. Detection limit was determined using 3σ/s method. Energy transfer efficiency was calculated as E = 1 - FDA/FD. Statistical analysis included mean and standard deviation for cell imaging.