1：Experimental Design and Method Selection:

The study employed a one-pot solvothermal method to synthesize the MSNs-CDs nanohybrid using folic acid and amino-modified mesoporous silica nanoparticles (MSNs-NH2) via microwave-assisted reaction. The design focused on in situ carbonization of folic acid on MSNs to retain folate receptor targeting capability.

2：Sample Selection and Data Sources:

Human cervical cancer cells (HeLa), human breast cancer cells (MCF-7), human lung adenocarcinoma cells (A549), and mouse fibroblast cells (L929) were used as cell models. Chemicals were purchased from commercial sources like Sinopharm Chemical Reagent Co., Ltd. and Aladdin Chemistry Co., Ltd.

3：List of Experimental Equipment and Materials:

Equipment included MDS-6G microwave chemical reactor (SMART, Sineo Microwave Chemistry Technology), transmission electron microscope (Tecnai F20), FT-IR spectrometer (Nicolet 6700), fluorescence spectrophotometer (Hitachi F-4600), XPS spectrograph (Axis Ultra DLD), DLS equipment (Malvern Zetasizer Nano ZS), XRD diffractometer (Rigaku D/max-2000), microplate reader (iMark 168-1130, Bio-Rad), and confocal laser scanning microscope (TSCSPS II, Leica). Materials included TEOS, CTAC, TEA, TMS-EDA, FA, DMSO, DOX, Hoechst 33258, MTT, DMEM, FBS, etc.

4：Experimental Procedures and Operational Workflow:

Synthesis involved preparing MSNs-NH2, then MSNs-CDs via solvothermal reaction, followed by DOX loading and release studies. Cell culture, cytotoxicity assays (MTT), targeting studies (CLSM and flow cytometry), and therapeutic efficacy tests were conducted with controlled conditions (e.g., pH, temperature).

5：Data Analysis Methods:

Data were analyzed using BET method for surface area, BJH model for pore size, TGA for weight loss, FT-IR and XPS for functional groups, fluorescence spectra for optical properties, DLS for size and zeta potential, and statistical analysis for cell viability and fluorescence intensity.