- 标题
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Intriguing “chameleon” fluorescent bioprobes for the visualization of lipid droplet-lysosome interplay
摘要： The interplay of lipid droplets (LDs) and lysosome plays an important role in cell metabolism, and the visualization of this process can provide useful information of organelle communication and function. However, fluorescent bioprobes based on organic fluorophores that can respond to LD-lysosome interplay are much rare. Herein, fluorescent bioprobes with high photostability, excellent biocompatibility and intracellular polarity sensitivity are achieved by encapsulating a new red fluorogenic molecule TPA-BTTDO within polymeric matrix (DSPE-PEG2000). They can sequentially localize in lysosome and LDs with red and cyan emissions, respectively. By monitoring the emission color change, the interesting dynamic processes of the probes escaping from lysosome and then enriching in LDs, and finally returning to lysosome after LDs consumption are visualized. In addition, the tracing of dynamic movement and consumption of LDs is realized by the probes with a high signal-to-noise ratio. The unique labeling behaviors and distinguished dual emissions of the probes in LDs and lysosome make them promising agents for fluorescence visualization studies of LD-lysosome related bioprocess and metabolism diseases.
关键词： lipid droplet,aggregation-induced emission,nanomaterials,lysosome,fluorescent bioprobe
更新于2025-11-21 11:08:12
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AIE active fluorescent organic nanoaggregates for selective detection of phenolic-nitroaromatic explosives and cell imaging
摘要： Development of organic nanoparticles with high fluorescence, good biocompatibility along with strong resistance to photobleaching through simple synthetic routes is important for diverse applications such as sensing and bioimaging. Herein, we present the development of a pyrene excimer nanoaggregate which shows aggregation induced emission (AIE) effect in a solvent mixture of THF and water. The pyrene based fluorescent probe, dimethyl-5-(pyren-1-ylmethyleneamino)isophthalate (5-DP) was synthesized through a simple single step condensation reaction from inexpensive reagents. The photophysical studies of nanoaggregated system further corroborates the AIE active behavior of 5-DP probe at different water fractions (?w = 0% to 90%), where the hydrogen bonding interaction between imine and water molecules led to suppression of photoinduced electron transfer (PET) inducing significant enhancement in fluorescence. The highly photostable nanoaggregates were explored as a selective fluorescence “turn off” sensor for phenolic nitroaromatics and the chemo-selectivity was highly pronounced for 2,4,6-trinitrophenol (picric acid), that showed efficient quenching in aqueous as well as solid phase, with a detection limit of 10 nM in aqueous medium. The quenching efficiency of the nanoaggregates can be ascribed to a combination of factors including efficient fluorescence resonance energy transfer, inner filter effect and coulombic interaction between picric acid and the aggregated probe molecules. Further, random aggregation of the pyrene derivative could be controlled for the formation of fluorescent spherical nanoparticles using Pluoronics P-123 block copolymers as encapsulating agents. The resulting composite could be used as a neoteric cell imaging probe with significantly less cytotoxicity, thus showing their potential biological applications.
关键词： aggregation induced emission,electron transfer,explosive detection,cell imaging,Fluorescent organic nanoaggregates
更新于2025-11-21 11:03:13
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Plasmon-Enhanced Fluorescent Sensor based on Aggregation-Induced Emission for the Study of Protein Conformational Transformation
摘要： The alteration in protein conformation not only affects the performance of its biological functions, but also leads to a variety of protein-mediated diseases. Developing a sensitive strategy for protein detection and monitoring its conformation changes is of great significance for the diagnosis and treatment of protein conformation diseases. Herein, a plasmon-enhanced fluorescence (PEF) sensor is developed, based on an aggregation-induced emission (AIE) molecule to monitor conformational changes in protein, using prion protein as a model. Three anthracene derivatives with AIE characteristics are synthesized and a water-miscible sulfonate salt of 9,10-bis(2-(6-sulfonaphthalen-2-yl)vinyl)anthracene (BSNVA) is selected to construct the PEF–AIE sensor. The sensor is nearly non-emissive when it is mixed with cellular prion protein while emits fluorescence when mixed with disease-associated prion protein (PrPSc). The kinetic process of conformational conversion can be monitored through the fluorescence changes of the PEF–AIE sensor. By right of the amplified fluorescence signal, this PEF–AIE sensor can achieve a detection limit 10 pM lower than the traditional AIE probe and exhibit a good performance in human serum sample. Furthermore, molecular docking simulations suggest that BSNVA tends to dock in the β-sheet structure of PrP by hydrophobic interaction between BSNVA and the exposed hydrophobic residues.
关键词： fluorescence sensor,protein conformation,aggregation-induced emission,prion protein,plasmonic enhancement
更新于2025-11-19 16:46:39
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Sensitive surface-enhanced Raman scattering detection of atrazine based on aggregation of silver nanoparticles modified carbon dots
摘要： The development of precise detection methods with simply operation for pesticides in various environmental samples is a particular challenge. Here a highly sensitive surface-enhanced Raman scattering (SERS) approach for the selective detection of trace atrazine was proposed with R6G as a Raman reporter, which was adsorbed on silver nanoparticles modified carbon dots. The latter were prepared by the reduction of Ag+ by carbon dots. In the presence of atrazine, the aggregation of the modified carbon dots due to the interaction between silver nanoparticles and atrazine led to great enhancement of the SERS signal of R6G. Under optimal assay conditions, the limit of quantification was estimated to be 10 nM, which matched with the standard for drinking water quality of China and WHO defined limit. A good linear response to atrazine was found in the concentration range of 10-1000 nM with the relative standard deviations between 1.8% and 5.6%. The determination of atrazine in real water samples was also carried out to confirm the practicability of the proposed method, which showed the recoveries from 95% to 117.5%. The target induced aggregation for enhancing the signal offered great potential for sensitive on-site detection of atrazine in environments.
关键词： Silver nanoparticles,Pesticides,SERS detection,Target induced aggregation,Atrazine,Carbon dots
更新于2025-11-14 17:03:37
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Fluorometric determination and intracellular imaging of cysteine by using glutathione capped gold nanoclusters and cerium(III) induced aggregation
摘要： A turn-on fluorometric method is described for selective and sensitive detection of cysteine (Cys). Gold nanoclusters (Au NCs) capped with glutathione (GSH) are used as a fluorescent probe. If Ce3+ ion are present, they will bind to the carboxy groups of the GSH-capped Au NC. This results in aggregation-induced emission enhancement (AIEE), best measured at excitation/emission wavelengths of 360/575 nm. On addition of Cys, which has less steric hindrance compared with GSH and higher affinity for Ce3+, it will bind to Ce3+ through the carboxyl group and link with Au NCs via Au-S bond. Hence, the AIEE is increased and Cys can be quantified via this effect with a linear response in the 0.4–120 μmol L?1 Cys concentration range and a detection limit of 0.15 μmol L?1.
关键词： Au NC,HepG2 cell imaging,Aggregation-induced emission enhancement
更新于2025-11-14 15:26:12
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Aggregation-induced emission (AIE)-active highly emissive novel carbazole-based dyes with various solid-state fluorescence and reversible mechanofluorochromism characteristics
摘要： Three new carbazole-based fluorescent molecules 1-3 functionalized with tetraphenylethene have been successfully synthesized, and these compounds have high thermal stability, and they exhibited different fluorescence in solid states with the superior luminescence quantum yields of 99.04% (1), 98.90% (2) and 39.83% (3). Their aggregation-induced behaviors were explored by the study of photoluminescence spectroscopy. The results showed that luminogens 1-3 exhibited remarkable aggregation-induced emission effect. Furthermore, their distinct mechanical stimulus-responsive fluorescence characteristics were also surveyed by solid-state photoluminescence spectroscopy. Interestingly, the various emitting colors of these luminogens could be changed into the same green, and the repeatabilities of their mechanochromic luminescence behaviors were outstanding, and the powder X-ray diffraction results indicated that the reversible conversion from a crystalline to an amorphous state was responsible for the obvious mechanofluorochromism phenomena of compounds 1-3. This work will be valuable for the exploitation of mechanical-force sensors with typical aggregation-induced emission feature.
关键词： Carbazole,Mechanofluorochromism,Tetraphenylethene,Aggregation-induced emission,Different fluorescence,High thermal stability
更新于2025-11-14 15:23:50
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The Self-Assembly of Cu-In-S Quantum Dots with Aggregation-Induced Emission into 3D Network Triggered by Cation and Its Application as A Novel Metal-Enhanced Fluorescent Nanosensor for Detecting Zn (Ⅱ)
摘要： A novel self-assembly phenomenon triggered by Zn2+ of Cu-In-S quantum dots with aggregation-induced emission effect was presented in this paper. Hydrophilic Cu-In-S quantum dots with aggregation-induced emission effect were successfully prepared. They were monodisperse spherical nanoparticles with the diameter of 2.8 ± 0.4 nm and had weak fluorescence in aqueous solution. However, the solution emitted strong fluorescence after addition of Zn2+. The results of TEM and SEM indicated the monodisperse quantum dots self-assembled into 3D networks of Cu-In-S quantum dots-Zn2+, which hindered the motion of quantum dots. Besides, the Zn2+ in the mixture passivated the surface defects. The phenomenon also proved by florescence lifetime and XPS. Thus the radiation decay decreased and followed by strong fluorescent emission. Interestingly, the degree of aggregation was proportional to the amount of Zn2+ and the fluorescent intensity. Based on this interesting phenomenon, a novel metal enhanced fluorescent nanosensor for detecting Zn2+ was established. The results demonstrated the proposed method had a good selectivity and linearity in the concentration range of 0-800 nmol?L-1 with a limit of detection of 1.99 ppb. These results showed a promising future in the field of metal-enhanced fluorescent probes of the Cu-In-S quantum dots.
关键词： Surface defects,Metal-enhanced sensor,Quantum dots,Restriction of motion,Aggregation-induced emission
更新于2025-11-14 15:23:50
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Multi-stimuli Responsive Supramolecular Gels Based on a D-π-A Structural Cyanostilbene Derivative with Aggregation Induced Emission Properties
摘要： Developing multi-stimuli responsive fluorescent gel materials in a single system remains challenging. Gelator molecules with classical fluorophores suffer from aggregation-caused quenching (ACQ) effect, limiting their applications further. Herein, a novel V-shaped cyanostilbene-based molecule (BAPBIA) with aggregation induced emission (AIE) characteristics and great gelation ability was synthesized and was found to exhibit multi-stimuli responsive behaviors. Reversible gel-sol phase transitions together with emission quenching are realized in response to external stimuli including heating, light and fluoride ion. Especially, the introduction of dimethylaniline group (donor) and cyano group (acceptor) generates a D-π-A structure, further leading to an intramolecular charge transfer (ICT) effect, which enlarges the emission contrast with the variations of the distribution of charge. Thus, upon trifluoroacetic acid (TFA) triggered protonation of dimethylaniline group, not only gel-sol transition but also emission color switching (yellow-to-blue) is achieved due to the loss of the ICT effect. This work paves an easy way to construct fully reversible multi-stimuli responsive fluorescence modulation smart materials.
关键词： supramolecular gels,aggregation induced emission,multi-stimuli responsive,fluorescence switching,cyanostilbene derivative
更新于2025-11-14 15:14:40
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Highly Efficient Luminescent Liquid Crystal with Aggregation-Induced Energy Transfer
摘要： A luminescent liquid crystal molecule (TPEMes) with efficient solid-state emission is rationally constructed via the chemically conjugation of blue-emitting tetraphenylethene cores and luminescent mesogenic tolane moieties, which are both featured with aggregation-induced emission properties. As for this fluorophore, aggregation induced energy transfer from the emissive tolane mesogens to the lighting-up tetraphenylethene units endows the molecule pure blue emission in the suspension and bulk state. Combining DSC, POM and 1D XRD experiments, TPEMes is deduced to adapt thermodynamically more stable layered crystalline phase, and can be 'frozen' into a monotropic smectic mesophase due to kinetic reasons. As a result of more densely packing of TPEMes in the crystalline phase indicated by 1D XRD, the luminescence of TPEMes in crystalline phase has blue-shifted with 17 nm relative to the metastable mesophase.
关键词： tolane,tetraphenylethene,aggregation induced emission,aggregation induced energy transfer,hexagonal columnar phase,luminescent liquid crystals
更新于2025-09-23 15:23:52
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Circularly Polarized Luminescence from Chiral Conjugated Poly(carbazole-ran-acridine)s with Aggregation-Induced Emission and Delayed Fluorescence
摘要： Pure organic materials with circularly polarized luminescence (CPL) and delayed fluorescence have gained increasing interest from academic and technological areas. To enhance the solid-state emission and exciton utilization in chiral optoelectronic devices, a synthetic strategy to impart conjugated polymers with CPL and aggregation-induced delayed fluorescence was proposed. Herein, two conjugated macromolecules with electron-donating poly(carbazole-ran-acridine) backbones, electron-withdrawing dibenzothiophen-2-yl(phenyl)methanone and chiral alanine moieties were designed and synthesized. Their neat films exhibited strong green emission with quantum yields of 6.7% and 10.3% and delayed fluorescence with lifetime of 1.358 μs and 1.366 μs, respectively. Both Cotton effect and CPL with dissymmetry factor of –2.01 × 10–3 and –1.39 × 10–3 were determined in the solid state. Such unique conjugated polymers were employed as solution-processed emitting layers in organic light emitting diodes, which displayed maximum brightness of 1477 cd/m2 and maximum current efficiency of 2.52 cd/A.
关键词： OLEDs,conjugated polymers,aggregation-induced emission,delayed fluorescence,aggregation-induced delayed fluorescence,circularly polarized luminescence
更新于2025-09-23 15:23:52