1：Experimental Design and Method Selection:

The study designed a SERS-based detection method using silver nanoparticles modified carbon dots (CD@Ag) as the substrate, with R6G as a Raman reporter. The principle involves target-induced aggregation of CD@Ag by atrazine, leading to enhanced SERS signals due to the formation of electromagnetic 'hot-spots'.

2：Sample Selection and Data Sources:

Real water samples were collected from Yi Sea in Anhui Jianzhu University, filtered, and spiked with atrazine at concentrations of 5, 10, or 200 nM for validation.

3：List of Experimental Equipment and Materials:

Materials included silver nitrate, sucrose, ethylenediamine, R6G, 3-mercaptopropionic acid (MPA), ethambutol (EB), atrazine, and ultrapure water. Equipment used were Cary 60 UV-vis spectrometer, Cary Eclipse fluorescence spectrometer, PerkinElmer FTIR spectrophotometer, Tecnai G2 F20 TEM, Thermo ESCALAB 250Xi XPS spectrometer, confocal microscopic Raman spectrometer (Renishaw), FEI Quanta 200 FEG SEM, and FLS980 Edinburgh fluorescence spectrometer.

4：Experimental Procedures and Operational Workflow:

CDs were synthesized hydrothermally from sucrose and ethylenediamine. CD@Ag was prepared by reducing Ag+ with CDs. For SERS detection, a mixture of R6G, CD@Ag, and atrazine was prepared, spotted on a quartz plate, and analyzed using a Raman microscope with a 633 nm laser at 0.2 mW power, 2 s integral time, and 25 mm slit aperture.

5：2 mW power, 2 s integral time, and 25 mm slit aperture. Data Analysis Methods:

5. Data Analysis Methods: SERS signals were analyzed for intensity changes with atrazine concentration. Linear regression was used for calibration curves, and limits of detection and quantification were estimated based on IUPAC recommendations.