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Silicon nanowire luminescent sensor for cardiovascular risk in saliva
摘要： Cardiovascular diseases are some of the today major cause of death in the world. C-reactive protein (CRP) is well known as the main biomarker related to cardiovascular risk and heart attack occurrence. The standard CRP analyses are performed in a hospital or in a biochemical laboratory with blood analysis after a long chemical and labelling preparation that require expert personnel. In this scenario, a health care analysis that can be performed by the same patient at his own home appears extremely revolutionary. In this paper, the study of an innovative sensing platform based on the luminescence at room temperature of silicon nanowires (NWs) is reported. This NWs sensor is label-free and does not require a chemical treatment of the analyte, is strongly selective to the CRP demonstrating a femtomolar limit of detection and a wide operating range. This proposed silicon sensing platform can be realized with an industrial compatible approach and permits to reveal the strategic CRP level in saliva in order to prevent a heart attack, with great advantages for the patient.
关键词： Cardiovascular risk,Saliva,C-reactive protein,Silicon nanowires,Luminescent sensor
更新于2025-11-21 11:01:37
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Silanized quantum dots as labels in lateral flow test strips for C-reactive protein
摘要： The paper describes the first use of silanized semiconductor core-shell quantum dots as fluorescent labels for macromolecule, C-reactive protein determination in blood plasma. The controlled synthesis of CdSe cores, with successive shells of CdS, CdZnS, ZnS and coating with transparent, stable, and inert silica shell, provides quantum dots with a narrow emission band, high quantum yield, and prolonged signal stability. Finally, the quantum dots were conjugated with specific antibodies via carboxylic groups on the silica surface. The method was further used for the immunochromatographic assay of C-reactive protein, a diagnostically important inflammatory biomarker. Assays with both the fluorescent QDs and a widely used colloidal gold label were developed in parallel and compared. The silanized quantum dots provide a more sensitive assay with a detection limit of 1 ng/mL for C-reactive protein in standard solutions, whereas the common assay has a detection limit of 10 ng/mL. The possibility of quantitative evaluation of analyte content by a portable device was demonstrated; the accuracy of the measurements was in the range of 5%–10%. The tests were used to determine C-reactive proteins in human plasma samples. The selected optimized protocol for these samples is based on a 4-fold dilution. The final working range of the assay, 4–1,200 ng/mL, covers practically all important interval of C-reactive protein values for the characterization of acute, chronic, and local inflammatory processes. Due to their high physical stability and inertness as well as intense, stable, and reproducible fluorescence, silanized quantum dots may be applied for high-sensitive assays for different analytes.
关键词： C-reactive protein,Quantum dots,silanization,lateral flow immunoassay
更新于2025-11-19 16:46:39
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ZnO/porous carbon composite from a mixed-ligand MOF for ultrasensitive electrochemical immunosensing of C-reactive protein
摘要： Carbon-based nano-composite materials obtained via simple thermolysis of metal-organic framework (MOF) have competitive virtues in accordance of layered porosity, controllable morphologies, and easily functionalizing with other metal/metallic oxides or hetero atoms. These make them directly as high activity catalysts or supports for various electrochemical sensor. Herein, we developed a novel ZnO/porous carbon matrix (ZnO/MPC)-based electrochemical immunosensing through thermolysis of a mixed-ligand MOF (Zn-BDC-TED) for real sample analysis of C-reactive protein. The ZnO/MPC and ionic liquid (IL) composite membrane modified electrode displayed prominent conductivity and biocompatibility for ultrasensitive detection of C-reactive protein (CRP). Subsequently, the step-by-step assembly process of the CRP immunosensor was monitored by electrochemical measurements including cyclic voltammogram (CV) and electrochemical impedance spectra (EIS). After the parameter optimization, the differential pulse voltammetry (DPV) response decreased linearly with the logarithm of CRP levels in an extensive range of 0.01–1000 ng·mL?1, and the detection limit was as low as 5.0 pg·mL?1. Meanwhile, the lable-free immunosensor also showed excellent selectivity, reproducibility and stability. We believe that ZnO/MPC composite and many carbon-based nano-composite materials obtained via simple thermolysis of MOF will be a prospective platform for sensitive detecting other biomarkers.
关键词： ZnO/MPC,C-reactive protein,Electrochemical immunosensor,Pyrolysis,Lable-free
更新于2025-09-23 15:22:29
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2D-Porphrinic Covalent Organic Framework-Based Aptasensor with Enhanced Photoelectrochemical Response for the Detection of C-Reactive Protein
摘要： In this study, a novel photoelectrochemical (PEC) aptasensor based on two-dimensional (2D) porphyrinic covalent organic frameworks (p-COFs) for the label-free detection of C-reactive protein (CRP) is presented. The obtained p-COFs possess high conductivity and an improved stability due to strong and rigid covalent linkages. The introduction of p-COFs hinder the recombination of electrons and holes, decreasing their band gap (Eg), thereby which improved the photocurrent conversion efficiency. Compared with pure porphyrin, p-COFs exhibited enhanced photocurrent intensity. An amplified photocurrent conversion efficiency and enhanced photocurrent results from H2O2, which act as active molecules and electron donors. As an unprecedented application of COFs in PEC bioanalysis, the detection of CRP with a PEC aptasensor is presented. The assembly of a CRP aptamer on the surface of Ag nanoparticles hinders the electron transfer, resulting in the decrease of the photocurrent response. This PEC aptasensor exhibits good analytical performances such as a rapid response, high stability, wide linear range and excellent selectivity, making COFs promising candidates for PEC bioanalysis.
关键词： C-reactive protein,Porphyrinic covalent organic framework,High photocurrent conversion efficiency,Photoelectrochemical aptasensor,Enhanced photocurrent
更新于2025-09-23 15:22:29
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A New Device Based on Interferometric Optical Detection Method for Label-Free Screening of C-Reactive Protein
摘要： In previous work, the performance of a compact and cost-effective point-of-care (PoC) device based on the increase relative optical power (IROP) methodology is reported and it is determined the enhancement in comparison with standard high-resolution spectrometry in terms of limit of detection. This paper describes a new label-free IROP-based biomedical device capable of working with low concentration of reagents and low sample volume per measurement in order to be used for screening different steps necessary in immunoassay optimization. This new approach significantly improves the sensing performance in terms of read-out signal (ΔIROP) per nanometer of biofilm in comparison with our previous work. This improvement is achieved due to the implementation of a laser as light source of the optical read-out system and the redesign of Fabry–Perot transducers by optimizing their reflectivity response and reducing their sensing area. For demonstrating the screening capability of this new PoC device in several immunoassays steps and methodologies, a C-reactive protein detection assay was carried out as a potential application and assay model. It is remarkable that only 10 μL of sample was used per measurement. This label-free IROP-based device complies an easy-to-use and cost-effective tool for immunoassays optimization in terms of performance, reagents cost, and measuring time.
关键词： C-reactive protein (CRP),label-free optical screening,immunoassays,point-of-care (PoC) devices,biosensors
更新于2025-09-10 09:29:36