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- 摘要
- 关键词
- 实验方案
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Flow cytometry and micro-Raman spectroscopy: Identification of hemocyte populations in the mussel Mytilus galloprovincialis (Bivalvia: Mytilidae) from Faro Lake and Tyrrhenian Sea (Sicily, Italy)
摘要： Immunological and structural characteristics of hemocyte populations in the mussel Mytilus galloprovincialis (Bivalvia: Mytilidae), going from two different Sicilian habitats (Faro Lake and Tyrrhenian sea), was investigated by means of two different techniques (flow cytometric and micro-Raman spectroscopy analyses). For this purpose, three hundred and sixty mussels Mytilus galloprovincialis were analyzed during November 2017. They were divided into two equal groups (triplicate sample) on the basis of the site of collection (n = 60 caught in Faro Lake - group A, and n = 60 caught in Tyrrhenian Sea - group B). Some several differences between the species of Faro Lake and Tyrrhenian Sea are observed and ascribed to the disruption of immune parameters induced by the variations of some qualitative water parameters (temperature, salinity, dissolved oxygen, pH, ammonium 10, free chlorine, total chlorine, total phosphate, orthofhosphate) recorded in the two habitats. This study is relevant for monitoring the conditions of the sea and Faro Lake, which is strongly influenced by the currents of the Tyrrhenian Sea. Faro lake is well known for the cultivation of mussels and this is part of a coastal habitat of particular interest, consisted of a peculiar biocenotic complex. Further, for the first time, significant different arrangement in the mussels cell structural organization was evidenced by simply following their highly reproducible Raman biomolecular signatures.
关键词： Hemocyte,Bivalve immunology,Deep-sea,Mussel Mytilus galloprovincialis,Flow cytometry,Raman spectroscopy
更新于2025-09-23 15:23:52
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[IEEE 2018 OCEANS - MTS/IEEE Kobe Techno-Ocean (OTO) - Kobe, Japan (2018.5.28-2018.5.31)] 2018 OCEANS - MTS/IEEE Kobe Techno-Oceans (OTO) - Monitoring and Communication System Design for A Deep-Sea Unmanned Submersible
摘要： Underwater vehicle is an important equipment for ocean monitoring and exploitation, and has been attracting more researchers and practitioners. Deep-sea unmanned submersible is a special type of underwater vehicle. This paper designs the monitoring and communication system for a deep-sea unmanned submersible using LABVIEW. The monitoring system includes initialization interface module, state display module and remote intervene module. The communication system consists of WIFI communication module, iridium communication module, underwater acoustic communication, and communication protocol between the monitoring computer on the surface ship and the submersible. The designed monitoring and communication system will be very helpful for operating the submersible in a safe and efficient way.
关键词： monitoring,LABVIEW,underwater acoustic communication,deep-sea unmanned submersible
更新于2025-09-23 15:22:29
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[IEEE 2018 OCEANS - MTS/IEEE Kobe Techno-Ocean (OTO) - Kobe, Japan (2018.5.28-2018.5.31)] 2018 OCEANS - MTS/IEEE Kobe Techno-Oceans (OTO) - A Dimension-Reduced Modal Space Detector in Deep-Sea Environment
摘要： The matched correlation detector (MCD), combining the received data with the sound transfer function (namely the replica field) is theoretically optimal for underwater passive detection. However, it suffers from the model mismatch problem caused by environmental uncertainties. And furthermore, when applied to the deep-sea environment, it encounters the large search range problem. In this paper, we focus on the source detection problem in deep-sea environment. To overcome the disadvantages of MCD, we choose the modal space detector (MSD) which uses a vertical linear array (VLA). We derive the expression of the deep-sea MSD and further, to improve the signal-to-noise ratio (SNR) of the test statistic, we propose a dimension-reduced form of MSD, which is termed as DR-MSD for short. By numerical simulation, we discuss that how source frequency, array depth and array aperture influence the dimension reduction. And we point out that the dimension-reduced number in DR-MSD decreases when the source frequency and the VLA aperture increase. The numerical results also indicate that DR-MSD can alleviate the search burden and obtain a better detection performance when compared to traditional MSD.
关键词： underwater acoustic,passive source detection,Deep-sea environment,dimension reduction,MSD
更新于2025-09-23 15:22:29
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[IEEE 2018 OCEANS - MTS/IEEE Kobe Techno-Ocean (OTO) - Kobe (2018.5.28-2018.5.31)] 2018 OCEANS - MTS/IEEE Kobe Techno-Oceans (OTO) - Detection of Small Hydrothermal Vents by Low-Altitude Seafloor Exploration of a Hovering-Type AUV “Hobalin”
摘要： We have used a hovering-type AUV (Autonomous Underwater Vehicle), “Hobalin” for investigation of hydrothermal fields. Data of deep seafloor images and water temperature sensor were obtained. Both optical observations and water sensor measurements were valid for detection of small hydrothermal vents, and water temperature distribution near seafloor was efficient for estimation of their positions.
关键词： deep sea,seafloor,hydrothermal vent,AUV
更新于2025-09-23 15:22:29
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[IEEE 2019 2nd World Conference on Mechanical Engineering and Intelligent Manufacturing (WCMEIM) - Shanghai, China (2019.11.22-2019.11.24)] 2019 2nd World Conference on Mechanical Engineering and Intelligent Manufacturing (WCMEIM) - Deep Sea Laser Scanning System for Underwater In-Situ Measurement
摘要： In this paper, a novel laser scanning system for deep sea in-situ measurement was developed. Firstly, the main components of the prototype were introduced separately. Then, the performance of the system was tested and analyzed in a tank filled with water with a standard ball bar. Results showed that the laser scanning system in water can achieve high precision. In addition, the prototype was placed in deep sea to verify the performance of the in-situ measurement and the measured targets are well reconstructed.
关键词： high precision,laser scanning system,in-situ measurement,deep sea
更新于2025-09-23 15:19:57
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Quantitative in situ mapping of elements in deep-sea hydrothermal vents using laser-induced breakdown spectroscopy and multivariate analysis
摘要： This study describes a method to quantify the chemical composition of deep-sea hydrothermal deposits in situ using laser-induced breakdown spectroscopy (LIBS). Partial least squares (PLS) regression analysis is applied to spectra obtained using a long laser pulse with a duration of 150 ns. The number of measurements needed to address the spatial heterogeneity of samples is determined through high-resolution mapping of the elemental distribution in rock samples. PLS applied to laboratory measured seawater-submerged samples achieved an average relative error (RE) of 25% for Cu, Pb, and Zn compared to benchmark concentration values in cross-validation and validation studies, where both the benchmark concentration values and LIBS spectral data are made available with this publication. The PLS model was applied to LIBS signals obtained in situ from hydrothermal deposits at 1000 m depth in the ocean. The results show that target inhomogeneity limits the accuracy of the surface LIBS measurements compared to benchmark values from bulk analysis of samples. Making multiple measurements with small position offsets at each location improves the accuracy of estimates compared to an equivalent number of measurements at a single position. Maps of element distribution generated using quantified in situ data demonstrate how chemical survey outputs can be generated by combining LIBS with multivariate analysis. This enables real-time chemical feedback during deep-sea operations and chemical surveys in situations or with platforms where sample recovery is not possible.
关键词： Multivariate analysis,Laser-induced breakdown spectroscopy (LIBS),Deep-sea explorations,In situ chemical analysis,Seafloor mineral resources,Partial least squares regression analysis
更新于2025-09-23 15:19:57
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[IEEE 2019 18th International Conference on Optical Communications and Networks (ICOCN) - Huangshan, China (2019.8.5-2019.8.8)] 2019 18th International Conference on Optical Communications and Networks (ICOCN) - Research on all-fiber deep sea pressure sensor
摘要： Theoretically analyzed and experimentally demonstrated an all-fiber pressure sensor based on a microfiber coupler combined SAGNAC loop. The work of this paper proves the usability of the structure in the deep sea environment.
关键词： deep sea pressure sensor,SAGNAC loop,All-fiber sensing,microfiber coupler
更新于2025-09-16 10:30:52
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Spectral characteristics of underwater laser-induced breakdown spectroscopy at high pressure conditions
摘要： Laser-induced breakdown spectroscopy (LIBS) has been proven to be an attractive technique for the in-situ oceanic applications. However, when applying LIBS into deep-sea, the pressure effect caused by different ocean depth is inescapable and could have great influence on the LIBS signals. In this work, spectral characteristics of underwater LIBS were investigated as a function of pressure in the range of 0.1–45 MPa. A high-pressure chamber built in the laboratory was used to simulate the high-pressure environment of deep-sea. Optimal laser energy and detection delay were first determined under different pressure conditions and were shown to be independent on the external pressure. The increase in pressure has a significant impact both on the peak intensity and line broadening of the observed spectra. The peak intensity of Na, Li and K lines increases with the increasing pressure until a maximum intensity reached at 12.5 MPa. Above this value, the peak intensity decreases gradually up to 45 MPa. For Ca line, the maximum intensity was observed at 30 MPa. The line broadening keeps constant at low pressures from 0.1 to 10 MPa while it increases linearly at higher pressures, indicating a higher electron density caused by the compression effect of the high external pressure. We also compared the spectral data obtained from the high-pressure chamber and from the field sea trials, and the good consistency between the laboratory data and sea-trial data suggested the key role of pressure effect on underwater LIBS signals for the practical deep-sea applications.
关键词： high-pressure chamber,underwater laser-induced breakdown spectroscopy,deep-sea application,pressure effect,spectral characteristics
更新于2025-09-16 10:30:52
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Anatomy of the free tantulus larva (Crustacea: Tantulocarida) studied with confocal laser scanning microscopy: An extreme case of miniatuarisation in the Arthropoda
摘要： The Tantulocarida is a small taxon of microscopic parasitic crustaceans that has a sophisticated life-cycle. The unique larval stage is called a tantulus, which is adapted to both free-swimming in sediments and attached parasitic mode of life. The anatomy of free-swimming tantulus larvae was studied using confocal laser scanning microscopy (CLSM) for the first time. This revealed previously undocumented details of the internal organisation of the Tantulocarida. Structures known to degrade after larval settlement were observed intact for the first time. The cement glands appeared to consist of six large, spherical cells with relatively large nuclei. The body muscles, proboscis and stylet retractors and protractors were able to be described in detail, whilst the central nervous system, including the compact brain and ventral nerve cord, were observed for the first time. This study has revealed the changes that occur in the internal organisation of the larvae after settlement, and provides a new understanding of certain general aspects of tantulocarid anatomy. The morphological adaptations to parasitism and the relatively minute size of these crustaceans are elucidated. Several aspects of arthropod miniaturisation are illustrated, using tantulocarids as examples. The potential for CLSM in studies of rare, microscopic arthropods is discussed.
关键词： Morphological adaptations,Microanatomy,Miniaturization,Deep sea,CLSM,Tantulocarida,Parasitic crustaceans
更新于2025-09-16 10:30:52