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- 摘要
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Application of a Fluorescence Imaging System with Indocyanine Green to Protect the Parathyroid Gland Intraoperatively and to Predict Postoperative Parathyroidism
摘要： Identifying the parathyroid gland during surgery may be difficult for surgeons. It is critical for them to be able to locate it during thyroidectomy or parathyroidectomy because of the significant function of the parathyroid in the calcium balance. According to recent reports, intrinsic fluorescence of the parathyroid has been found. There is some evidence to suggest that new equipment can detect the fluorescence via imaging technology. In this study, a newly invented intraoperative fluorescence imaging system and indocyanine green (ICG) dye were applied to detect the parathyroid glands and evaluate the vascularization of the parathyroid.
关键词： Postoperative parathyroidism,Indocyanine green,Parathyroid,Fluorescence imaging system
更新于2025-09-10 09:29:36
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Comprehensive Supramolecular Chemistry II || Fluorescent Sensors for Biological Metal Ions
摘要： All living organisms require metals. They have vital roles as catalysts, osmotic regulators, signaling molecules, and transport molecules. Many different metals are present in the human body, and carry out a range of essential functions: for example, oxygen travels in the blood bound to iron in hemoglobin, neurons communicate by changing their sodium and potassium levels, and metal catalysts like manganese in superoxide dismutase speed up biochemical reactions.1 Although metals are so essential, they make up less than 3% of the human body by weight. The most abundant metals found in the body belong to periodic groups I and II, including calcium (1.5%), potassium (0.4%), sodium (0.2%), and magnesium (0.1%). Essential transition metals are considered to be trace elements because they collectively make up less than 0.1% of body mass. These include zinc, chromium, vanadium, cobalt, copper, manganese, molybdenum, and tin.2,3
关键词： Fluorescent Sensors,Biological Metal Ions,Fluorescence Imaging,Metal Probes,Ratiometric Probes
更新于2025-09-10 09:29:36
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DNA-Stabilized Silver Nanoclusters for Label-Free Fluorescence Imaging of Cell Surface Glycans and Fluorescence Guided Photothermal Therapy
摘要： A multifunctional nanoplatform that enables the integration of biological detection, imaging diagnosis, and synergistic therapy into a single nanostructure holds great promise for nanoscience and nanomedicine. Herein, a novel theranostic platform was presented for label-free imaging of cell surface glycans based on DNA/silver nanoclusters (AgNCs) via hybridization chain reaction (HCR) and fluorescence guided photothermal therapy (PTT). In this strategy, a dibenzocyclooctyne (DBCO)-functionalized DNA and two hairpin structures of DNA/AgNCs probes were involved. Following metabolic glycan labeling, the binding of DBCO-functionalized DNA to cell surface initiated HCR, and then cell surface glycans were specifically labeled by DNA/AgNCs fluorescent probes. Furthermore, this signal amplification strategy was adopted in quantitative analysis, and the detection limit could be achieved as low as 20 cells in 200 μL binding buffer. Moreover, the remarkable photothermal properties of DNA/AgNCs via HCR, led to efficient killing of cancer cell and inhibited the tumor growth under imaging guide. In this strategy, DNA/AgNCs were utilized to detect the cellular glycans, which aided in overcoming the high cost and instability of fluorescent dyes. Simultaneously, the HCR process avoided the introduction of excessive azido-sugars under the precondition of ensuring apparent fluorescence. These results indicated that the developed nanoplatform has great potential for specific cell surface glycans imaging and fluorescence guided PTT.
关键词： DNA-stabilized silver nanoclusters,fluorescence guided photothermal therapy,hybridization chain reaction,cell surface glycans,label-free fluorescence imaging
更新于2025-09-09 09:28:46
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A curcumin-based NIR fluorescence probe for detection of amyloid-beta (Aβ) plaques in Alzheimer's disease
摘要： Amyloid-beta (Aβ) peptide as one of the main components of senile plaques is closely related to the onset and progression of incurable Alzheimer's disease (AD). In recent years, it is reported that curcumin derivatives have been used as the near-infrared (NIR) fluorescence imaging probes of Aβ plaques for the early diagnosis of AD. To further develop a curcumin-based NIR fluorescent probe for Aβ plaques, in this work, we have synthesized three novel curcumin-based NIR Dyes. Among them, the curcumin derivative Dye 2 showed a significant enhancement in its fluorescence intensity (λem, 635 nm; 19.5-fold increase in quantum yield, Φ = 0.36; Kd, 1.13 μM) after binding to Aβ plaques. Additionally, in vitro and in vivo fluorescence imaging of Aβ plaques strained with Dye 2 confirmed that the compound was a potential probe to detect Aβ plaques in AD. This work opens a perspective to rationally design novel curcumin-based NIR compound for Aβ detection.
关键词： Alzheimer’s Disease,Fluorescence imaging,Amyloid-beta deposits,NIR fluorescence probe,Curcumin,Amyloid-beta plaques
更新于2025-09-09 09:28:46
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An NIR-II Fluorescence/Dual Bioluminescence Multiplexed Imaging for In Vivo Visualizing the Location, Survival, and Differentiation of Transplanted Stem Cells
摘要： The in vivo distribution, viability, and differentiation capability of transplanted stem cells are vital for the therapeutic efficacy of stem cell–based therapy. Herein, an NIR-II fluorescence/dual bioluminescence multiplexed imaging method covering the visible and the second near-infrared window from 400 to 1700 nm is successfully developed for in vivo monitoring the location, survival, and osteogenic differentiation of transplanted human mesenchymal stem cells (hMSCs) in a calvarial defect mouse model. The exogenous Ag2S quantum dot–based fluorescence imaging in the second near-infrared window is applied for visualizing the long-term biodistribution of transplanted hMSCs. Endogenous red firefly luciferase (RFLuc)-based bioluminescence imaging (BLI) and the collagen type 1 promoter–driven Gaussia luciferase (GLuc)-based BLI are employed to report the survival and osteogenic differentiation statuses of the transplanted hMSCs. Meanwhile, by integrating the three imaging channels, multiple dynamic biological behaviors of transplanted hMSCs and the promotion effects of immunosuppression and the bone morphogenetic protein 2 on the survival and osteogenic differentiation of transplanted hMSCs are directly observed. The novel multiplexed imaging method can greatly expand the capability for multifunctional analysis of the fates and therapeutic capabilities of the transplanted stem cells, and aid in the improvement of stem cell–based regeneration therapies and their clinical translation.
关键词： near-infrared fluorescence imaging,bioluminescence imaging,transplanted stem cells,stem cell fate,regenerative medicine
更新于2025-09-09 09:28:46
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Fast confocal fluorescence imaging in freely behaving mice
摘要： Fluorescence imaging in the brain of freely behaving mice is challenging due to severe miniaturization constraints. In particular, the ability to image a large field of view at high temporal resolution and with efficient out-of-focus background rejection still raises technical difficulties. Here, we present a novel fiberscope system that provides fast (up to 200 Hz) background-free fluorescence imaging in freely behaving mice over a field of view of diameter 230 μm. The fiberscope is composed of a custom-made multipoint-scanning confocal microscope coupled to the animal with an image guide and a micro-objective. By simultaneously registering a multipoint-scanning confocal image and a conventional widefield image, we subtracted the residual out-of-focus background and provided a background-free confocal image. Illumination and detection pinholes were created using a digital micromirror device, providing high adaptability to the sample structure and imaging conditions. Using this novel imaging tool, we demonstrated fast fluorescence imaging of microvasculature up to 120 μm deep in the mouse cortex, with an out-of-focus background reduced by two orders of magnitude compared with widefield microscopy. Taking advantage of the high acquisition rate (200 Hz), we measured red blood cell velocity in the cortical microvasculature and showed an increase in awake, unrestrained mice compared with anaesthetized animals.
关键词： red blood cell velocity,multipoint-scanning confocal microscope,digital micromirror device,fiberscope system,microvasculature imaging,fluorescence imaging,freely behaving mice
更新于2025-09-09 09:28:46
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[IEEE 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference (NSS/MIC) - Atlanta, GA (2017.10.21-2017.10.28)] 2017 IEEE Nuclear Science Symposium and Medical Imaging Conference (NSS/MIC) - Three-Dimensional Radiopharmaceutical-Excited Fluorescence Imaging of Lymph Nodes
摘要： Optical imaging techniques have been developed for localizing lymph nodes before surgical resection due to the non-invasion and high sensitivity. However, its attendant penetrability limitations and auto-fluorescence effect have greatly limited the spatial resolution and imaging precision. In this study, a novel technique radiopharmaceutical-excited fluorescence imaging (REFI) was adopted to image lymph nodes, which use gamma-ray and Cerenkov radiation from radioisotopes to excite lanthanide europium oxide (EO) nanophosphors and boost the light intensity. An effective adaptive-steepest-descent-projection onto convex sets (ASD-POCS) reconstruction algorithm and the anatomic structure information were used to three-dimensionally image lymph nodes in mice models. The results indicate that REFI can greatly boost the light intensity and accuracy of three-dimensional imaging of lymph node with location deviation less than 1.03 mm.
关键词： lymph nodes imaging,3D reconstruction,radiopharmaceutical-excited fluorescence imaging
更新于2025-09-09 09:28:46
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Raman and fluorescence imaging on polyoxometalate composite agarose films
摘要： Composite agarose films were prepared with Eu(III) containing polyoxometalates, in particular the organic-inorganic hybrid [Eu(W5O18)2(pic)4]13- (Hpic = 3-hydroxypicolinic acid) and a Mo blue Eu(III) derivative. The latter compound shows interesting Eu(III) based photoluminescent properties, firstly described for a molybdenum blue compound. Imaging of the films optical properties and morphology was explored by Raman and fluorescence measurements in a confocal microscopy system, together with the AFM profile. Raman and fluorescence imaging were powerful tools for the characterization of the films, in particular concerning its chemical composition and homogeneity at the micrometric scale.
关键词： Fluorescence imaging,Agarose films,Polyoxometalates,Raman imaging,Europium
更新于2025-09-09 09:28:46
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A Novel Fluorescence Switch for MicroRNA Imaging in Living Cells based on DNAzyme Amplification Strategy.
摘要： MicroRNAs (miRNAs) play important roles in the regulation of target gene expression and cell development. Therefore, developing of accurate and visual detection methods for miRNAs is important for early diagnosis of cancer. In this study, we established a visual detection method for miRNA 155 based on DNAzyme amplification strategy in living cells. MnO2 nanosheets were employed to deliver Locked DNAzyme and Substrate DNA into cells. AuNPs-Probe were taken up by cells autonomously. Then, MnO2 nanosheets were reduced to Mn2+ by glutathione (GSH) in cells and DNA modules were released. MiRNA 155 took away Locker DNA by strand displacement reaction to activate the DNAzyme. Then the DNAzyme cleaved substrate DNA and released single-stranded DNA named Key DNA. Key DNA opened the hairpin DNA that modified on gold nanoparticles (AuNPs) and turn on the fluorescence of cy5. One target miRNA led to plenty of released Key DNA when lots of substrate DNA were added. Thus, the visual detection of miRNA 155 in living cells would be initiated. Under confocal laser microscopy, the fluorescence was obviously observed in tumor cells but not in normal cells. The method has a linear range from 0.1 nM to 10 nM and a low detection limit of 44 pM in vitro detection.
关键词： DNA walker,microRNA,AuNPs,Fluorescence imaging,MnO2,DNAzyme,Signal amplification
更新于2025-09-09 09:28:46
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P2.16-39 The Application of 3D Medical Image Analyzer and a Fluorescence Guided Surgery for Pulmonary Sublobar Resection
摘要： The advantages of this method are applicable to any type of sublobar resection, initial determination of resection area at operation, possible super deep wedge resection without broncho-vascular transection with enough margin, long identification of fluorescence, and indication in case of COPD, IP, reoperation and adhesion. On the other hand, the drawbacks are the necessity of a near infrared thoracoscopy and 3D medical image analyzer, knowledge of precise bronchial anatomy and advanced manipulation skills of bronchoscopy, ununiformity of ICG distribution and distribution of ICG into the adjacent area with the passage of time.
关键词： sublobar resection,three dimension image analysis,fluorescence imaging
更新于2025-09-09 09:28:46