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Impact of photodynamic therapy versus ultrasonic scaler on gingival health during treatment with orthodontic fixed appliances
摘要： Objectives: Poor oral hygiene during treatment with fixed appliances results in plaque accumulation. The presence of bacteria in the gingival crevice triggers an inflammatory reaction in the gingival tissues. The aim of this study was to compare the impact of two preventive treatments, photodynamic therapy (PDT), and ultrasonic scaler (US), on gingival health in patients under fixed orthodontic treatment. Methods: Twenty orthodontic patients were randomly allocated to two groups: PDT or US. Each group received seven sessions [days 0, 15, 30, 45, 90 (3-months follow-up), 180 (6-months follow-up), 270 (9-months follow-up)] of experimental interventions, and clinical parameters [Plaque index(PI); gingival index(GI); probing depth(PD)], periodontopathogens [Aggregatibacter actinomycetemcomitans; Porphyromonas gingivalis; Prevotella intermedia; Micromonas micros; Fusobacterium nucleatum; Tannerella forsythia; Campylobacter rectus; Eikenella corrodens; Capnocytophaga sp.] and protein markers [IL-1b;IL-1ra;IL-6;IL-10; TNF-a;FGF-2/FGF basic] were monitored at baseline and at 3, 6, and 9 months. ANOVA, Student’s t-test with Bonferroni correction and ANOVA with multiple rank test were used to identify differences between groups (P < 0.05). Results: Clinical assessments [PI, GI, and PD] yielded no differences (P > 0.05) between groups, which showed a major decrease at the start of the trial. Reductions in total colony forming units (log CFU reduction) were observed with both treatments, although to a greater extent in the PDT group, but with no differences between groups (P > 0.05). Similar reductions in log CFU counts of P. gingivalis, P. intermedia, and F. nucleatum were observed in both groups (P > 0.05). The two groups also showed similar trends for inflammatory mediators with decreased levels of IL-1b, IL-10, and TNF-a, whereas IL-6 and IL-1ra levels remained stable and those of FGF-2 were increased after both interventions, with no differences (P > 0.05) between groups. Conclusion: Both PDT and US methods proved similar effectiveness for the treatment of gingival inflammation induced by fixed orthodontic appliances.
关键词： photodynamic therapy,cytokines,ultrasonic scaler,orthodontic treatment,gingival inflammation,periodontopathogens
更新于2025-09-23 15:23:52
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Simulation study of the performance of new micropattern gaseous detectors
摘要： Purpose: ICAM-1 plays a critical role in the development of acute respiratory distress syndrome (ARDS). MK2 regulates the expression of ICAM-1 in human pulmonary microvascular endothelial cells. To explore whether the inhibition of MK2 activation has the same effect in experimental animals, MMI-0100, a peptide-mediated inhibitor of MK2, was used to verify whether MMI-0100 can ameliorate lung inflammation in a mouse model of ARDS by reducing endothelial expression of ICAM-1. Methods: In this study, C57BL/6 mice were randomly divided into three groups: a control group, an lipopolysaccharides (LPS) group, and an LPS plus MMI-0100 group. Mice were killed 24 hours after the administration of LPS and MMI-0100. The mouse lung tissue histopathology, wet/dry weight ratio (W/D), and the neutrophil count were used to measure the severity of lung inflammation in mice. The pulmonary microvascular endothelial cells (PMVECs) of the mice were isolated. The mRNA expression of ICAM-1 in mouse PMVECs was determined using RT-PCR, and the protein expression of MK2 and ICAM-1 in mouse PMVECs was analyzed using Western blotting and immunohistochemistry. Results: We found that the level of phosphorylated MK2 in the LPS plus MMI-0100 group was reduced. Compared with the LPS group, the LPS plus MMI-0100 group of mice showed less severe inflammation, including a lower W/D and neutrophil count. The mRNA and protein expression of ICAM-1 in the LPS group was significantly higher than in the control group in mouse PMVECs, and the ICAM-1 level was reduced after the administration of MMI-0100. Conclusion: These data indicate that MMI-0100 ameliorates lung inflammation in a mouse model of ARDS by reducing endothelial expression of ICAM-1.
关键词： pulmonary microvascular endothelial cell,inflammation,acute respiratory distress syndrome
更新于2025-09-23 15:23:52
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A Fluorescent Cocktail Strategy for Differentiating Tumor, Inflammation, and Normal Cells by Detecting mRNA and H <sub/>2</sub> O <sub/>2</sub>
摘要： Accurately distinguishing tumors from noncancerous inflammation and normal tissues is hugely significant for tumor diagnosis and therapy. However, tumor and inflammatory tissues have similar pathologic characteristics in their microenvironment, making differentiation very difficult. Here, a fluorescent cocktail nanoparticle capable of simultaneously detecting intracellular mRNA and H2O2 was designed to differentiate tumors from nontumor cells. To detect targeted mRNA in living cells, a DNA probe was generated using the fluorescence resonance energy transfer (FRET) principle. A pH-responsive amphiphilic polymer was synthesized to realize the transportation of the DNA probe. In addition, the polymer was conjugated with a coumarin-boronic acid ester (Cou-BE) H2O2 probe. According to the change in the fluorescence of Cou-BE, tumor and inflammatory cells could be distinguished from normal cells owing to their high concentration of H2O2. Because of the different concentrations of tumor-related mRNA in tumor and nontumor cells, the fluorescence intensity of the DNA probe-loaded nanoparticles inside tumor cells was different from that inside inflammatory cells. Therefore, our fluorescent cocktail strategy could discriminate simultaneously tumor, inflammation, and normal cells through the cooperative detection of intracellular mRNA and H2O2, which demonstrated potential application value in biomedical research and clinical diagnosis.
关键词： H2O2,tumor,tumor-related mRNA,fluorescence imaging,inflammation
更新于2025-09-23 15:23:52
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A self-illuminating nanoparticle for inflammation imaging and cancer therapy
摘要： Nanoparticles have been extensively used for inflammation imaging and photodynamic therapy of cancer. However, the major translational barriers to most nanoparticle-based imaging and therapy applications are the limited depth of tissue penetration, inevitable requirement of external irradiation, and poor biocompatibility of the nanoparticles. To overcome these critical limitations, we synthesized a sensitive, specific, biodegradable luminescent nanoparticle that is self-assembled from an amphiphilic polymeric conjugate with a luminescent donor (luminol) and a fluorescent acceptor [chlorin e6 (Ce6)] for in vivo luminescence imaging and photodynamic therapy in deep tissues. Mechanistically, reactive oxygen species (ROS) and myeloperoxidase generated in inflammatory sites or the tumor microenvironment trigger bioluminescence resonance energy transfer and the production of singlet oxygen (1O2) from the nanoparticle, enabling in vivo imaging and cancer therapy, respectively. This self-illuminating nanoparticle shows an excellent in vivo imaging capability with suitable tissue penetration and resolution in diverse animal models of inflammation. It is also proven to be a selective, potent, and safe antitumor nanomedicine that specifically kills cancer cells via in situ 1O2 produced in the tumor microenvironment, which contains a high level of ROS.
关键词： photodynamic therapy,cancer therapy,inflammation imaging,reactive oxygen species,myeloperoxidase,bioluminescence resonance energy transfer,nanoparticles
更新于2025-09-23 15:22:29
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Macrophage uptake switches on OCT contrast of superparamagnetic nanoparticles for imaging of atherosclerotic plaques
摘要： Background: Optical coherence tomography (OCT) is an intravascular, high-resolution imaging technique that is used to characterize atherosclerotic plaques. However, the identification of macrophages as important markers of inflammation and plaque vulnerability remains difficult. Here, we investigate whether the uptake of very small iron oxide particles (VSOP) in macrophages, that cluster in phagolysosomes and allow high-quality magnetic resonance imaging (MRI) of atherosclerotic plaques, and uptake of ferumoxytol nanoparticles enhance detection of macrophages by OCT. Materials and methods: RAW 264.7 macrophage cells were incubated with VSOP (1 and 2 mM Fe) that have been clinically tested and ferumoxytol (8.9 mM Fe) that is approved for iron deficiency treatment and currently investigated as an MRI contrast agent. The light scattering of control macrophages, nanoparticle-labeled macrophages (2,000,000 in 500 μL) and nanoparticle suspensions was measured in synchronous wavelength scan mode using a fluorescence spectrophotometer. For OCT analyses, pellets of 8,000,000 non-labeled, VSOP-labeled and ferumoxytol-labeled RAW 264.7 macrophages were imaged and analyzed on an OPTIS? OCT imaging system. Results: Incubation with 1 and 2 mM VSOP resulted in uptake of 7.1±1.5 and 12±1.5 pg Fe per cell, which increased the backscattering of the macrophages in spectrophotometry 2.5- and 3.6-fold, whereas incubation with 8.9 mM Fe ferumoxytol resulted in uptake of 6.6±2 pg Fe per cell, which increased the backscattering 1.5-fold at 700 nm. In contrast, backscattering of non-clustered nanoparticles in suspension was negligible. Accordingly, OCT imaging could visualize significantly increased backscattering and signal attenuation of nanoparticle-labeled macrophages in comparison with controls. Conclusion: We conclude that VSOP and, to a lesser extent, ferumoxytol increase light scattering and attenuation when taken up by macrophages and can serve as a multimodal imaging probe for MRI and OCT to improve macrophage detection in atherosclerotic plaques by OCT in the future.
关键词： intravascular,magnetic resonance imaging,multimodal imaging,optical coherence tomography,vulnerability,inflammation
更新于2025-09-23 15:22:29
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Fish oil preparation inhibits leukocyte recruitment and bands that characterize inflamed tissue in a model of phenol-induced skin inflammation: percutaneous penetration of a topically applied preparation demonstrated by photoacoustic spectroscopy
摘要： Fish oil (FO) is a natural source of omega-3 fatty acids, with well-established beneficial effects in inflammatory diseases when FO is orally administered. This study investigated the effects of a topically applied FO preparation (FOP) on phenol-induced ear edema and evaluated the percutaneous penetration of FOP in ear tissue. After applying phenol, groups of mice received FOP on the ear. After 1 h, ear tissue was collected to determine the percent inhibition of edema, myeloperoxidase activity, and to perform photoacoustic spectroscopy (PAS). Treatment with FOP did not reduce edema, but reduced myeloperoxidase activity. The FOP decreased the area of bands that characterize inflamed tissue and penetrated into the tissue. These results indicated an inhibitory effect of FOP on leukocyte recruitment in phenol-induced ear edema. These data support the applicability of PAS as a non-destructive method for evaluating the inflammatory response, percutaneous penetration and antiinflammatory activity of compounds.
关键词： ear edema,Fish oil,inflammation,phenol,skin,photoacoustic spectroscopy
更新于2025-09-23 15:22:29
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Dual-Modal In Vivo Fluorescence/Photoacoustic Microscopy Imaging of Inflammation Induced by GFP-Expressing Bacteria
摘要： In this study, dual-modal fluorescence and photoacoustic microscopy was performed for noninvasive and functional in vivo imaging of inflammation induced by green fluorescent protein (GFP) transfected bacteria in mice ear. Our imaging results demonstrated that the multimodal imaging technique is able to monitor the tissue immunovascular responses to infections with molecular specificity. Our study also indicated that the combination of photoacoustic and fluorescence microscopy imaging can simultaneously track the biochemical changes including the bacterial distribution and morphological change of blood vessels in the biological tissues with high resolution and enhanced sensitivity. Consequently, the developed method paves a new avenue for improving the understanding of the pathology mechanism of inflammation.
关键词： GFP-expressing bacteria,fluorescence imaging,biosensor,photoacoustic microscopy,inflammation/infection
更新于2025-09-23 15:22:29
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CdS nanoparticles of different lengths induce differential responses in some of the liver functions of mice
摘要： Cadmium sulfide nanoparticles (CdS NPs) are one of important nanoparticle materials which are widely used in photoelectric production, but their potential health hazard to the liver is not clear. This study is aimed at exploring the possible mechanisms of liver injury induced by CdS NPs. Male mice were treated with nanoparticles of 110–130 nm and 80–100 nm cadmium sulfide. The main methods were based on detecting the vigor of superoxide dismutase (SOD) and glutathione (GSH), and content of malondialdehyde (MDA) in both blood and liver tissues as well as on observing the pathological changes in liver tissue. CdS NPs suppressed the activity of SOD and GSH, and increased the serum MDA content (p < 0.05); both effects were observed together in liver tissues of 80–100 nm group (p < 0.05) and were accompanied by an obviously inflammatory response. CdS NPs induced oxidative damage and inflammatory response in liver tissue, which may be an underlying mechanism for its pulmonary toxicity. Additionally, the toxicity of CdS NPs was closely related to the size of nanoparticles. Pathological results showed that the hepatotoxicity of shorter CdS NPs is greater than that of longer CdS NPs (Tab. 6, Fig. 3, Ref. 20). Text in PDF www.elis.sk.
关键词： oxidative damage,cadmium sulfide nanoparticles,inflammation
更新于2025-09-23 15:22:29
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<sup>18</sup> F-Sodium Fluoride Positron Emission Tomography and Plaque Calcification
摘要： 18F-sodium fluoride (18F-NaF) was introduced as a tracer for imaging skeletal diseases in 1962 and was approved by the Food and Drug Administration in 1972.1 Recently, with the increased availability of positron emission tomography (PET) scanners there has been a surge in clinical utilization of 18F-NaF imaging for oncological applications. The incidental observation, nearly a decade ago, of 18F-NaF uptake in the vasculature in patients undergoing PET imaging for cancer has led to a growing number of investigations exploring the potential role of this tracer in atherosclerosis.2–4 However, the biological correlates of 18F-NaF imaging in the vasculature, and its potential role in risk stratification of patients and prospective identification of vulnerable plaques remain incompletely characterized. In this issue of the Journal, Creager et al5 address some of these gaps by exploring the relationship between 18F-NaF binding and the size of microcalcifications using a 3-dimensional hydrogel platform.6 In agreement with a previous publication,2 their study finds that smaller and more numerous microcalcifications (ie, higher surface areas of calcifications) are associated with higher 18F-NaF binding when compared with fewer larger calcifications.5 The study also provides ex vivo proof-of-concept evidence for the correlation between 18F-NaF binding and foci of ongoing calcification in mouse and human atherosclerotic plaques.5
关键词： Editorials,sodium fluoride,atherosclerosis,inflammation,positron emission tomography
更新于2025-09-23 15:22:29
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The background and philosophy behind daylight photodynamic therapy
摘要： Conventional photodynamic therapy (PDT) is associated with side effects, primarily related to the waiting time between pretreatment with application of photosensitizer and illumination. Pain during illumination is a major issue for the patients and options for effective pain relief are limited. Post-treatment inflammation can often be severe and cause inconvenient down-time for the patients and their employers. To avoid the problems of pain and patients crowding in the clinic we eliminated red light treatment of high PpIX concentration and introduced illumination in daylight which may be performed at home. We also investigated if protoporphyrin IX (PpIX) could be activated continuously during its formation which might reduce pain and inflammation. Continuous activation of PpIX during its formation turned out to minimize pain as single PpIX molecules are activated continuously without accumulation of PpIX in the skin. PpIX molecules are formed in the mitochondria and the photodynamic effect only takes place in the mitochondria when continuously activated. This results primarily in apoptosis with little inflammation. Continuous activation of PpIX can be obtained by performing photodynamic therapy in daylight, as well as with daylight-emitting light sources of appropriate wavelengths. Use of daylight prevents the patients from crowding in the clinic. Daylight-PDT completely fulfils the purpose of minimizing pain and inflammation, as well as limiting the strain on the clinic treating the patients.
关键词： Photochemotherapy,Pain,Inflammation,Protoporphyrin IX
更新于2025-09-23 15:22:29