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<p>Biodistribution and sensitive tracking of immune cells with plasmonic gold nanostars</p>
摘要： Aim: To quantitatively and sensitively investigate the biodistribution of immune cells after systemic administration. Methods: Immune cells were loaded with plasmonic gold nanostars (GNS) tracking probes. Inductively coupled plasma mass spectrometry (ICP-MS) was used for quantitative gold mass measurement and two-photon photoluminescence (TPL) was used for high-resolution sensitive optical imaging. Results: GNS nanoparticles were loaded successfully into immune cells without negative effect on cellular vitality. Liver and spleen were identified to be the major organs for macrophage cells uptake after systematic administration. A small amount of macrophage cells were detected in the tumor site in our murine lymphoma animal model. Conclusion: GNS has great potential as a biocompatible marker for quantitative tracking and high-resolution imaging of immune cells at the cellular level.
关键词： biodistribution,immune cells,GNS,ICP-MS,two-photon microscopy
更新于2025-11-19 16:56:42
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Zircon fissiona??track and Ua??Pb double dating using femtosecond laser ablationa??inductively coupled plasmaa??mass spectrometry: A technical note
摘要： We present a new LA–ICP–MS system for zircon fission-track (FT) and U–Pb double dating, whereby a femtosecond laser combined with galvanometric optics simultaneously ablates multiple spots to measure average surface U contents. The U contents of zircon measured by LA–ICP–MS and standardized with the NIST SRM610 glass are comparable to those measured by the induced fission track method, and have smaller analytical errors. LA–ICP–MS FT dating of seven zircon samples including three IUGS age standards is as accurate as the external detector method, but can give a higher-precision age depending on the counting statistics of the U content measurement. Double dating of the IUGS age standards gives FT and U–Pb ages that are in agreement. A chip of the Nancy 91500 zircon has a homogeneous U content of 84 ppm, suggesting the possibility of using this zircon as a matrix-matched U standard for FT dating. When using the Nancy 91500 zircon as a U standard, a zeta calibration value of 42–43 yr cm?2 for LA–ICP–MS FT dating is obtained. While this value is strictly only valid for the particular session, it can serve as a reference for other studies.
关键词： dating,femtosecond laser,U–Pb,fission track,zircon,LA–ICP–MS
更新于2025-09-23 15:21:01
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Spatially-resolved uranium isotopic analysis of contaminated scrap metal using laser ablation multi-collector ICP-MS
摘要： Laser ablation multi-collector inductively coupled plasma mass spectrometry (LA-MC-ICP-MS) was applied to the detailed investigation of the uranium (U) isotopic composition (234U, 235U, 236U, and 238U) of five contaminated scrap metal samples found within the European Union. Pressed pellets of the two certified U isotopic reference materials CRM U-020 and CRM U-030 were included in the measurement protocol for mass bias correction, calculation of the ion counter gains and for quality assurance. Since the investigated samples had low U content (0.15–14.3 wt%) compared to typically analysed pure U compounds (>60 wt%), the applied experimental parameters had to be adjusted. Spatially-resolved U isotopic information was obtained by line scan analysis of each sample. While other analytical techniques used typically in nuclear forensic investigations, such as g-spectrometry and thermal ionisation mass spectrometry (TIMS) yielded average U isotopic compositions of the entire sample, LA-MC-ICP-MS provided substantial added value, highlighting the inhomogeneous distribution of U isotopes within various scrap metal samples. Analysis of individual particles via secondary ion mass spectrometry (SIMS) confirmed the large range of 235U enrichment levels in heterogeneous scrap metal samples. Four out of five scrap metal samples contained 236U ((cid:1)0.05–(cid:1)0.11 wt%), indicating the presence of reprocessed U. Taken together, LA-MC-ICP-MS analysis provided fast and accurate spatially-resolved U isotopic information without consuming or altering the scrap metal samples, a key feature for nuclear forensics investigations.
关键词： nuclear forensics,Laser ablation,multi-collector ICP-MS,uranium isotopic analysis,scrap metal
更新于2025-09-23 15:21:01
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A high performance method for the accurate and precise determination of silicon isotopic compositions in bulk silicate rock samples using laser ablation MC-ICP-MS
摘要： MC-ICP-MS has become one of the most powerful analytical methods for Si isotopes in bulk liquid and solid samples in the past decade. Laser ablation provides direct sampling of solid samples, avoiding laborious chemical digestion procedures. As for powder bulk samples, the preparation of stable and homogeneous targets prior to measurements, and strategies to minimize potential matrix effects, are critical for accurate isotopic analysis. In this study, an accurate and precise method for Si isotope ratio measurements by fs-LA-MC-ICP-MS of bulk silicate rock samples was established. A new laser fusion sample preparation technique was developed to achieve fast and homogeneous fusion of silicate rock powders (including granite and granodiorite that contain abundant refractory minerals) into glasses. Compared to the pressed pellet technique, the measurement precision for fused glasses was improved ~5.7-fold. Matrix effects during Si isotopic analysis were observed when using non-matrix matched calibration protocols under normal dry-plasma instrument conditions with both ns- and fs-laser using a raster ablation mode. Matrix effects were eliminated by the addition of water vapor into the plasma, realizing accurate non-matrix matched calibration. In addition, fs-laser provided better measurement precisions, with the internal and external precisions being improved ~1.85 and ~2.4 fold compared to ns-laser at nearly equal signal intensities. Five well characterized silicate rock reference materials were analyzed using fs-LA-MC-ICP-MS calibrated against NBS-28 and BHVO-2G. Results obtained for =29Si and =30Si for these five reference materials were in agreement with previously reported values, confirming the accuracy of the proposed method.
关键词： matrix effects,Silicon isotopic analysis,water vapor addition,laser ablation MC-ICP-MS,silicate rock samples
更新于2025-09-23 15:21:01
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An Image Mapping Approach to U-Pb LA-ICP-MS Carbonate Dating, and Applications to Direct Dating of Carbonate Sedimentation
摘要： We present a new approach to laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) U-Pb dating of carbonates based on selection and pooling of pixels from 2D elemental and isotopic ratio maps. This image mapping technique is particularly useful for targeting sub-domains in samples with complex geological histories. Key major and trace elements that are sensitive to detrital components, post-formational fluid ingress, mineralogical changes or diagenetic overprinting are measured along with the Pb and U isotopic data. Laser sampling is undertaken along successive linear rasters that are compiled into maps using the Monocle add-on for Iolite, with one pixel in the map corresponding to one time-slice of the time-resolved signal. These element, element-ratio and isotope ratio maps can be overlain over photomicrographs or SEM images to spatially link compositional data to textural and structural features. The pixels corresponding to likely homogeneous age domains can be isolated by applying appropriate selection criteria (e.g., Th<0.3 ppm, Mg/Ca<0.004) and pooled into pseudo-analyses using a proxy for the parent/daughter ratio (e.g., 207Pb/235U, 238U/208Pb) to retrieve the largest possible spread of the data points on isochron diagrams. The approach is best suited for analytical setups capable of rapidly or simultaneously scanning over a large mass range, and can yield a precision of ±1 % or better on quadrupole instruments depending on U concentration, 238U/204Pb and age of the sample. The sample-specific filtering criteria for selection and rejection of data and their rationale can be reported, resulting in more transparency with regards to data processing.
关键词： image mapping,carbonates,Monocle,Iolite,LA-ICP-MS,U-Pb dating
更新于2025-09-23 15:21:01
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Fast offline data reduction of laser ablation MC-ICP-MS Sr isotope measurements via the interactive Excel-based spreadsheet a??SrDRa??
摘要： Strontium isotopes are applied to a wide range of scientific fields and to different types of sample materials, providing valuable information foremost about provenance and age, but also on diagenetic processes and mixing relationships between different Sr reservoirs. The development of in-situ analytical techniques, such as laser ablation ICP-MS, has improved our understanding of Sr isotope variability in several field of application, because of the possibility to discriminate small-scale changes and their spatial distribution. However, large outputs of Sr isotope data are produced by laser ablation MC-ICP-MS systems, which necessitate of multiple offline steps to correct and assess the data. This requires the availability of simple and user-friendly tools, easily manageable also by non-specialists. With this in mind, we developed SrDR, an Excel-based interactive data reduction spreadsheet (‘SrDR’, Sr-Data-Reduction) for the processing of Sr isotopes measured by LA-MC-ICP-MS. The SrDR spreadsheet is easily customizable (a) to meet user-specific analytical protocols, (b) for different instruments (i.e. Nu Plasma vs. Neptune), and (c) for diverse target materials (e.g. Rare Earth Elements enriched or depleted samples). We include also several examples relevant to low and high temperature geochemistry fields - a fossil tooth, a modern seashell, a speleothem sample and plagioclase crystals - to show how different sample materials are corrected for different interfering masses.
关键词： Strontium isotopes,SrDR,Excel spreadsheet,data reduction,LA-MC-ICP-MS
更新于2025-09-23 15:19:57
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Tracing multi-isotopically labelled CdSe/ZnS quantum dots in biological media
摘要： The strengths and limits of isotopically labelled engineered nanoparticles (spiked ENPs) spread in biological media have been assessed. Multi-spiked CdSe/ZnS quantum dots (QDs), measuring 7 nm and coated with thioglycolic acid (TGA), were synthesized and enriched in 68Zn, 77Se and 111Cd. These QDs were dispersed at very low concentrations (0.1 to 5000 ppt) in diverse biological matrices (synthetic saliva, synthetic urine, plasma and Dulbecco’s phosphate buffered saline - DPBS growth medium) and the isotopic compositions were determined by HR-ICP-MS. The initial QDs concentrations were calculated to assess the limit of quantification (QD-LOQ) according to the matrix and the isotopically enriched element. The obtained results demonstrated the advantages of the isotopic labelling method in order to work at very low concentrations: the QD-LOQ values for the spiked Zn, Cd and Se originated from the QDs were 10, 0.3 and 6 ppt, respectively, which is below the conventional LOQ of the HR-ICP-MS used (30, 3 and 60 ppt for Zn, Cd and Se, respectively). Conversely, in complex matrices such as saliva, urine, plasma and DPBS growth medium, the QD-LOQ values increased significantly, with values ranging from 16 to 32 ppt for Cd, 446 to 10598 ppt for Zn and 1618 to 8317 ppt for Se. These QD-LOQs are dependent on factors as the elemental background concentration already present in the matrices, and the dilution factor. In this study, the QD-LOQs are expressed for the first time with respect to the background concentration in biological media (QD-RLOQ), which can be used to better assess and then predict the efficiency of the spiking method.
关键词： HR-ICP-MS,biological media,isotopically labelled,limit of quantification,quantum dots
更新于2025-09-23 15:19:57
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a??Blind timea?? a?? current limitations on laser ablation multi-collector inductively coupled plasma mass spectrometry (LA-MC-ICP-MS) for ultra-transient signal isotope ratio analysis and application to individual sub-micron sized uranium particles
摘要： The application of laser ablation multi-collector inductively coupled plasma mass spectrometry (LA-MC-ICP-MS) to the isotope ratio analysis of UOx particles has the potential to improve the isotopic determination of these particles when compared to currently utilised ICP-MS techniques. To investigate this a high-speed, integrated ablation cell and dual concentric injector design, was tested in the expectation that the resulting increase in signal to noise ratio and sample ion yield would improve the determination of 234U/238U, 235U/238U and 236U/238U for such materials. However, when compared to a slower washout, more established low-volume cell design, the highly transient signals of the new design proved challenging for the mixed detector array of the multi-collector mass spectrometer, introducing a new bias. We describe a major component of this bias, referred to as ‘blind time’, and model its impact on UOx particle analysis. After accounting for blind time, average precisions for the uranium isotopic composition of sub-micron sized UOx particles using LA-MC-ICP-MS were 3 % 1RSD for 235U/238U and 8 % 1RSD for 234U/238U. When ablating a glass rather than a UOx particle, uncertainties of 1.3 % 1RSD for 235U/238U were achieved for 150 nm equivalent particle sizes using LA-MC-ICP-MS.
关键词： isotope ratio analysis,UOx particles,uranium isotopic composition,LA-MC-ICP-MS,blind time
更新于2025-09-23 15:19:57
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Determination of Metal Concentration in Road-Side Trees from an Industrial Area Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry
摘要： Historical pollution can be elucidated with variations of elements’ concentration in tree rings by using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). However, the capacity of chemical elements’ absorption significantly depends on the tree species and element types. Metal concentrations in the rings for five species (Platanus occidentalis, Salix koreensis, Chamaecyparis obtusa, Pinus densiflora, Ginkgo biloba) were investigated in light of metal pollution history in ambient air of D industrial site located in Daejeon, Korea. The calibration for LA-ICP-MS was performed using cellulose-matrix matched standards with 13C normalization. Tree ring series except for Ginkgo sp. showed that the accumulation rates of Pb and Cd were higher between 1992 and 1999. Other elements, such as Fe, Cr, Mn, Cd, Zn, and Sr, showed a variation in the rings, likely due to the different physiological processes of element uptake and radial mobility. Concentrations of Pb and Cd in the annual rings of Pinus sp. corresponded to the metal monitoring data for the ambient air with the correlation coefficients of 0.879 and 0.579, respectively. Moreover, Cd in Platanus sp. and Pb in Salix sp. showed a positive correlation to ambient metal concentration compared to Chamaecyparis sp. and Ginkgo sp. Therefore, caution should be taken to select candidate elements as well as tree species to reconstruct the ambient air metal pollution history by measuring the concentration of metal in the tree ring.
关键词： Environmental pollution history,Tree ring,Dendrochemistry,LA-ICP-MS
更新于2025-09-23 15:19:57
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Nanoparticle enhanced laser ablation and consequent effects on laser induced plasma optical emission
摘要： In this paper the plasmon enhanced ablation for elemental analysis is investigated with several experiments in order to point out the crucial questions concerning the laser matter interaction under the effect of plasmonic coupling between the nanoparticle (NP) system and the laser ablation pulse. The correlation between the electromagnetic field enhancement and the signal enhancement during NP enhanced laser induced breakdown spectroscopy (NELIBS), as well as the laser matter interaction at the nanoscale, is discussed in the case of noble metal NPs deposited on metal samples. The results suggest that, while laser pulse energy is concentrated in the space between the NPs, the NP system is shielded by the field enhancement distribution after the laser pulse interacts with the plasmons of the NP system. Finally the comparison of the laser energy transfer to the sample between NELIBS and conventional LIBS is discussed.
关键词： LIBS,Nanoparticle enhanced laser induced breakdown spectroscopy (NELIBS),laser ablation,plasmonic enhanced ablation,LA-ICP-MS
更新于2025-09-23 15:19:57