- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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Multifunctional Thermosensitive Liposomes Based on Natural Phase Change Material: Near-Infrared Light-Triggered Drug Release and Multimodal Imaging Guided Cancer Combination Therapy
摘要： Multifunctional theranostic nanoplatforms (NPs) in response to environment stimulations for on-demand drug release are highly desirable. Herein, the near-infrared (NIR)-absorbing dye, indocyanine green (ICG) and the antitumor drug, doxorubicin (DOX) were efficiently co-encapsulated into the thermosensitive liposomes based on natural phase change material (PCM). Folate and conjugated gadolinium chelate-modified liposome shells enhance active targeting and magnetic resonance (MR) performance of the NPs while maintaining the size of the NPs. The ICG/DOX loaded and gadolinium chelates conjugated temperature-sensitive liposomes nanoplatforms (ID@TSL-Gd NPs) exhibited NIR-triggered drug release and prominent chemo-, photothermal, photodynamic therapy properties. With the co-encapsulated ICG, DOX and the conjugated gadolinium chelates, the ID@TSL-Gd NPs can be used for triple-modal imaging (fluorescence/photoacoustic/magnetic resonance imaging, FL/PAI/MRI) guided combination tumor therapy (chemotherapy, photothermotherapy and photodynamic therapy, Chemo/PTT/PDT). After tail vein injection, the ID@TSL-Gd NPs accumulated effectively in subcutaneous HeLa tumor of mice. The tumor was effectively suppressed by accurate imaging guided NIR triggered phototherapy and chemotherapy, and no tumor regression and side effects were observed. In summary, the prepared ID@TSL-Gd NPs achieved multimodal imaging-guided cancer combination therapy, providing a promising platform for improving diagnosis and treatment of cancer.
关键词： Multimodal imaging,Liposomes,Thermosensitive,Combination therapy,Phase change material
更新于2025-11-21 11:08:12
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Simultaneous enhancement of red upconversion luminescence and CT contrast of NaGdF <sub/>4</sub> :Yb,Er nanoparticles <i>via</i> Lu <sup>3+</sup> doping
摘要： To date, lanthanide-doped upconversion nanoparticles (UCNPs) have been widely reported as a promising CT contrast agent because they have high atomic numbers and big X-ray attenuation coefficient values. However, it is still a challenge to fabricate a simple multimodal imaging probe with improved image quality for early cancer diagnosis in clinical medicine. Herein, ultra-small, uniform and monodisperse β-NaGdF4:Yb,Er,X% Lu (X = 0, 1, 2.5, 4, 6, 7.5) UCNPs were prepared through a solvothermal method with high-level modulation of both the phase and morphology. Meanwhile, a remarkably enhanced red upconversion luminescence (UCL) in the β-NaGdF4:Yb,Er,X% Lu NPs was successfully realized via Lu3+ doping. It is found that as the content of Lu3+ increases from 0 to 7.5 mol%, the UCL intensity of the red emission first increases and then decreases, with the optimum doping content of Lu3+ ions of 2.5 mol%. The red UCL enhancement is ascribed to the change of the Yb–Er interionic distance controlling the Yb–Er energy transfer rate and the distortion of the local environment of Er3+ ions influencing the 4f–4f transition rates of Er3+ ions, which has been further confirmed by the experimental check of the crystallographic phase and by photoluminescence spectroscopy employing Eu3+ as the structural probe, respectively. More importantly, after being modified with the HS-PEG2000-NH2 ligand, the NH2-PEGylated-NaGdF4:Yb,Er,X% Lu NPs exhibited low cytotoxicity, high biocompatibility, and remarkably enhanced contrast performance in in vitro UCL and in vivo CT imaging. On the basis of our findings, the as-obtained functionalized UCNPs could be considered as a promising versatile dual-mode imaging probe for bioimaging, tumor diagnosis, and cancer therapy.
关键词： red luminescence enhancement,Lu3+ doping,upconversion nanoparticles,multimodal imaging,CT contrast
更新于2025-11-14 17:04:02
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Spectral correction for handheld optoacoustic imaging by means of near-infrared optical tomography in reflection mode
摘要： In vivo imaging of tissue/vasculature oxygen saturation levels is of prime interest in many clinical applications. To this end, the feasibility of combining two distinct and complementary imaging modalities was investigated: optoacoustics (OA) and near-infrared tomography (NIROT), both operating noninvasively in reflection mode. Experiments were conducted on two optically heterogeneous phantoms mimicking tissue before and after the occurrence of a perturbation. OA imaging was used to resolve submillimetric vessel-like optical absorbers at depths up to 25 mm, but with a spectral distortion in the OA signals. NIROT measurements were utilized to image perturbations in the background and to estimate the light fluence inside the phantoms at the wavelength pair (760 nm, 830 nm). This enabled the spectral correction of the vessel-like absorbers' OA signals: the error in the ratio of the absorption coefficient at 830 nm to that at 760 nm was reduced from 60%-150% to 10%-20%. The results suggest that oxygen saturation (SO2) levels in arteries can be determined with <10% error and furthermore, that relative changes in vessels' SO2 can be monitored with even better accuracy. The outcome relies on a proper identification of the OA signals emanating from the studied vessels.
关键词： fluence compensation,near-infrared optical tomography,blood oxygen saturation,quantitative optoacoustic imaging,optoacoustic signal quantification,multimodal imaging,spectral correction
更新于2025-09-23 15:23:52
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Multimodal hard x-ray imaging with resolution approaching 10 nm for studies in material science
摘要： We report multimodal scanning hard x-ray imaging with spatial resolution approaching 10 nm and its application to contemporary studies in the field of material science. The high spatial resolution is achieved by focusing hard x-rays with two crossed multilayer Laue lenses and raster-scanning a sample with respect to the nanofocusing optics. Various techniques are used to characterize and verify the achieved focus size and imaging resolution. The multimodal imaging is realized by utilizing simultaneously absorption-, phase-, and fluorescence-contrast mechanisms. The combination of high spatial resolution and multimodal imaging enables a comprehensive study of a sample on a very fine length scale. In this work, the unique multimodal imaging capability was used to investigate a mixed ionic-electronic conducting ceramic-based membrane material employed in solid oxide fuel cells and membrane separations (compound of Ce0.8Gd0.2O2?x and CoFe2O4) which revealed the existence of an emergent material phase and quantified the chemical complexity at the nanoscale.
关键词： mixed ionic-electronic conducting membrane,x-ray nanoscale imaging,multimodal imaging,high spatial resolution
更新于2025-09-23 15:23:52
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Development of a sub-miniature gamma camera for multimodal imaging system
摘要： In the recent past, gamma-ray imaging detectors have achieved an intrinsic spatial resolution of less than 1 mm within a few centimeters of a useful field of view (UFOV). Unlike to conventional gamma cameras, which are large and heavy, the compact gamma-ray imaging detectors can improve the performances of the gamma cameras used in the various fields. In this study, we developed a sub-miniature gamma camera for a multimodal imaging system. The camera has a gamma-ray detector, miniature electronics modules, and a diverging hole collimator. The detector consisted of the sub-millimeter pixelated Ce:GAGG array and the silicon photomultiplier (SiPM) array module. We organized the miniature electronics modules according to the functions; an MPPC base board, analog signal processing board, integrated power supply board, and compact data acquisition (DAQ) base board. The diverging hole collimator widened an imaging area of the gamma camera from the UFOV of the detector. On the detector side, dimensions of each hole and septa were identical to the pixel and inter-pixel thickness of the reflector of scintillator array. For the intrinsic performance test, we acquired a flood map image of 729 (27 × 27) scintillator pixels, and the energy resolution was 18.9 % for an integrated energy histogram of 99mTc (140 keV). For the extrinsic performance test, we used the 57Co sheet source, and made a 99mTc line source using a capillary tube. The sources located at 10 cm apart from the collimator surface. The imaging area was three times wider than the UFOV of the detector. The system sensitivity was 19 CPM/μCi and the spatial resolution was 3.5 mm. The usability of the proposed gamma camera will not be confined to existing applications due to its compactness and novelty.
关键词： Sub-miniature gamma camera,Gamma camera performance evaluation,Front-end electronics,Multimodal imaging system,Diverging hole collimator
更新于2025-09-23 15:23:52
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Chemical and topographical single-cell imaging by near-field desorption mass spectrometry
摘要： Simultaneous acquiring chemical and topographical information within a single cell at nanoscale resolutions is vital to cellular biology, yet it remains a great challenge for current techniques due to limited lateral resolutions and detection sensitivities. Here we report the development of near-field desorption mass spectrometry for co-registered chemical and topographical imaging, thereby bridging the gap between laser-based MS methods and multimodal single-cell imaging. Using this integrated platform, imaging resolution of 250 nm and 3D topographically reconstructed chemical single-cell imaging were achieved. This technique offers more in-depth cellular information than is currently possible with micrometre-range laser-based MS imaging methods. Considering the simplicity and compact size of the near-field device, this technique can be introduced to matrix-assisted laser desorption ionization MS, expanding the multimodal abilities of MS at nanoscale resolutions across wide disciplines.
关键词： single-cell analysis,analytical methods,multimodal imaging,mass spectrometry,near-field desorption
更新于2025-09-23 15:23:52
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Carbon Nanomaterials for Bioimaging, Bioanalysis, and Therapy || Photoacoustic Imaging with Carbon Nanomaterials
摘要： Photoacoustic imaging is a novel, noninvasive biomedical imaging modality that has evolved considerably over the last few decades. As a label‐free imaging modality using both endogenous and exogenous contrast agent it has shown many advantages to safely and effectively differentiate diseased tissue from healthy tissues at a deeper depth. While endogenous light‐absorbing objects in living subjects such as hemoglobin, melanin, and glucose, have been useful in imaging, the use of exogenous contrast agents can improve the detection sensitivity and specific tissue‐targeting capabilities of photoacoustic imaging modality further. The carbon nanomaterial has been found to be one of the best contrast agents for photoacoustic imaging, which has strong absorption properties and great biocompatibility. This chapter covers the basic introduction of photoacoustic imaging and the application of carbon nanomaterials contrast agent such as imaging‐guided therapy and multimodal imaging in photoacoustic imaging.
关键词： photoacoustic imaging,contrast agents,photothermal therapy,photodynamic therapy,multimodal imaging,biomedical imaging,carbon nanomaterials
更新于2025-09-23 15:22:29
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Macrophage uptake switches on OCT contrast of superparamagnetic nanoparticles for imaging of atherosclerotic plaques
摘要： Background: Optical coherence tomography (OCT) is an intravascular, high-resolution imaging technique that is used to characterize atherosclerotic plaques. However, the identification of macrophages as important markers of inflammation and plaque vulnerability remains difficult. Here, we investigate whether the uptake of very small iron oxide particles (VSOP) in macrophages, that cluster in phagolysosomes and allow high-quality magnetic resonance imaging (MRI) of atherosclerotic plaques, and uptake of ferumoxytol nanoparticles enhance detection of macrophages by OCT. Materials and methods: RAW 264.7 macrophage cells were incubated with VSOP (1 and 2 mM Fe) that have been clinically tested and ferumoxytol (8.9 mM Fe) that is approved for iron deficiency treatment and currently investigated as an MRI contrast agent. The light scattering of control macrophages, nanoparticle-labeled macrophages (2,000,000 in 500 μL) and nanoparticle suspensions was measured in synchronous wavelength scan mode using a fluorescence spectrophotometer. For OCT analyses, pellets of 8,000,000 non-labeled, VSOP-labeled and ferumoxytol-labeled RAW 264.7 macrophages were imaged and analyzed on an OPTIS? OCT imaging system. Results: Incubation with 1 and 2 mM VSOP resulted in uptake of 7.1±1.5 and 12±1.5 pg Fe per cell, which increased the backscattering of the macrophages in spectrophotometry 2.5- and 3.6-fold, whereas incubation with 8.9 mM Fe ferumoxytol resulted in uptake of 6.6±2 pg Fe per cell, which increased the backscattering 1.5-fold at 700 nm. In contrast, backscattering of non-clustered nanoparticles in suspension was negligible. Accordingly, OCT imaging could visualize significantly increased backscattering and signal attenuation of nanoparticle-labeled macrophages in comparison with controls. Conclusion: We conclude that VSOP and, to a lesser extent, ferumoxytol increase light scattering and attenuation when taken up by macrophages and can serve as a multimodal imaging probe for MRI and OCT to improve macrophage detection in atherosclerotic plaques by OCT in the future.
关键词： intravascular,magnetic resonance imaging,multimodal imaging,optical coherence tomography,vulnerability,inflammation
更新于2025-09-23 15:22:29
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[IEEE 2018 International Conference on Intelligent Informatics and Biomedical Sciences (ICIIBMS) - Bangkok (2018.10.21-2018.10.24)] 2018 International Conference on Intelligent Informatics and Biomedical Sciences (ICIIBMS) - A Novel Tumor Imaging Agent, Tc-99m and Fluorescence Labeled Gastrin-Releasing Peptide Analogues
摘要： Statement of the Problem: A variety of imaging probes have been developed for different molecular targets. The gastrin-releasing peptide (GRP) analogues (GRP, GRP18-27 and GRP21-27) were investigated vigorously as a targeting peptide for tumor, however, the cancer targeting abilities of these GRP analogues had not been compared head-to-head. Direct comparison of characteristics of these analogues will provide a useful information for the design of molecular imaging agent associated with GRP and an insight within the mechanism of tumor targeting using GRP analogues. Approach: In the present study, we developed Tc-99m and fluorescence (carboxytetramethylrhodamine, TAMRA) labeled GRP analogues containing three different peptides (TAMRA-GHEG-ECG-GRP, TAMRA-GHEG-ECG-GRP18-27 and TAMRA-GHEG-ECG-GRP21-27) to target the tumor cells and compared the tumor targeting abilities using in vitro and in vivo experiments. Peptides were synthesized using Fmoc solid-phase peptide synthesis. Radiolabeling of peptides with Tc-99m was done using ligand exchange via tartrate. Binding affinity and in vitro cellular uptake studies were performed. Gamma camera imaging study was performed in murine models with PC-3 tumors. The average counts per pixel within the ROIs were measured and target-to-non-target ratios were calculated. Results: After radiolabeling procedures with Tc-99m, Tc-99m labeled peptides were prepared in high yield (>96%). Tc-99m TAMRA-GHEG-ECG-GRP (Kd = 7.9 ± 2.7 nM) showed highest binding affinity for PC-3 tumor cells and Tc-99m TAMRA-GHEG-ECG-GRP18-27 (Kd = 12.6 ± 3.6 nM) showed relatively low binding affinity. Confocal microscopy images of PC-3 cells incubated with TAMRA-GHEG-ECG-GRP and TAMRA-GHEG-ECG-GRP21-27 showed strong fluorescence in the cytoplasm. Gamma camera imaging revealed substantial uptake of Tc-99m TAMRA-GHEG-ECG-GRP21-27 in tumors. Conclusion: We developed three Tc-99m and TAMRA labeled GRP analogues as a molecular imaging agent for targeting tumor. In vitro studies demonstrated substantial binding affinity and cellular uptake of TAMRA-GHEG-ECG-GRP and TAMRA-GHEG-ECG-GRP21-27. In contrast, in vivo gamma imaging study revealed that only Tc-99m TAMRA-GHEG-ECG-GRP21-27 was significantly accumulated in the tumor tissue. Taken together, the present study suggest that 7-mer peptide, GRP21-27 is the best surrogate as a targeting ligand for tumor imaging.
关键词： Gastrin-releasing peptide,Bombesin,Tc-99m,TAMRA,Multimodal imaging
更新于2025-09-23 15:22:29
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Multimodal Imaging in a Case of Idiopathic Neuroretinitis
摘要： A 37-year-old woman presented with painful visual loss in the left eye for 2 weeks. The best-corrected visual acuity was 20/200. Ophthalmic examination of the left eye revealed vitreous cells, optic disc swelling, serous retinal detachment, and macular star-figure hard exudates. Swept-source optical coherence tomography showed both inner and outer retinas were swollen, the choroid was thickened, and the corresponding retinal pigment epithelium was elevated. Wide-field indocyanine green angiography disclosed multiple hypofluorescent spots in the mid-periphery. Taken together, an involvement of optic disc, entire retina, and choroid was considered in the current case.
关键词： Choroidal involvement,Multimodal imaging,Neuroretinitis
更新于2025-09-23 15:22:29