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Polydopamine Coated PB-MnO <sub/>2</sub> Nanoparticles as an Oxygen Generator Nanosystem for Imaging-Guided Single-NIR-Laser Triggered Synergistic Photodynamic/Photothermal Therapy
摘要： Exploring a combined phototherapeutic strategy to overcome the limitations of a single mode therapy and inducing high anticancer efficiency is highly promising for precision cancer nanomedicine. However, a single-wavelength laser activates dual photothermal/photodynamic therapy (PTT/PDT) treatment is still a formidable challenge. Herein, we strategically design and fabricate a multifunctional theranostic nanosystem based on chlorin e6-functionalized polydopamine (PDA) coated prussian blue/manganese dioxide nanoparticles (PB-MnO2@PDA-Ce6 NPs). the obtained PB-MnO2@PDA NPs not only offer an effective delivery system for Ce6 but also provide strong optical absorption in the near-infrared range, endowing high antitumor efficacy of PTT. More importantly, the as-prepared PB-MnO2@PDA-Ce6 nanoagents exhibit an effective oxygen generation, superior reactive oxygen species (ROS), and outstanding photothermal conversion ability to greatly improve PTT and PDT treatments. As a result, both in vitro and in vivo treatments guided by MR imaging on liver cancer cells reveal the complete cell/tumor eradication under a single wavelength of 660 nm laser irradiation, implying the simultaneous synergistic PDT/PTT effects triggered by PB-MnO2@PDA-Ce6 nanoplatform, which are much higher than individual treatment. Taken together, our phototherapeutic nanoagents exhibit an excellent therapeutic performance, which may act as a nanoplatform to find safe and clinically translatable routes to accelerate cancer therapeutics.
关键词： photodynamic therapy,nanomedicine,prussian blue,photothermal therapy,MRI,cancer therapy,polydopamine,chlorin e6,manganese dioxide
更新于2025-09-23 15:19:57
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685PA MULTICENTER PHASE II STUDY OF SALVAGE PHOTODYNAMIC THERAPY USING TALAPORFIN SODIUM AND A DIODE LASER FOR LOCAL FAILURE OF ESOPHAGEAL CANCER AFTER CHEMORADIOTHERAPY
摘要： We previously reported that photodynamic therapy (PDT) has a potential to cure local failure after chemoradiotherapy (CRT) for esophageal cancer (EC). However, first-generation PDT using porfimer sodium and an excimer dye laser has several problems: a high occurrence of skin phototoxicity, the requirement of a long light shielding period (6 weeks), and the need for an expensive and large laser generator. Talaporfin sodium (Laserphyrin?) is a second-generation photosensitizer that requires a shorter light shielding period (2 weeks) and induces less phototoxicity. We previously identified the optimum diode laser fluence (100 J/cm2) for PDT using talaporfin sodium in the phase I/IIA study. Therefore, in the present study, we conducted a multicenter phase IIB study to evaluate the efficacy and safety of PDT using talaporfin sodium as a salvage treatment for local failure after CRT or radiotherapy (RT) for EC. (UMIN000009184).
关键词： chemoradiotherapy,non-colorectal,esophageal cancer,photodynamic therapy,talaporfin sodium,diode laser,gastrointestinal tumours
更新于2025-09-23 15:19:57
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Prospective evaluation of changes in choroidal vascularity index after half-dose photodynamic therapy versus micropulse laser treatment in chronic central serous chorioretinopathy
摘要： Purpose To assess whether treatment of chronic central serous chorioretinopathy (cCSC) with photodynamic therapy (PDT) and high-density subthreshold micropulse laser (HSML) results in choroidal vascularity index (CVI) changes that may account for the treatment effect. Methods Patients with cCSC were prospectively included and analyzed. Patients received either half-dose PDT or HSML treatment. CVI of the affected and unaffected eye was obtained before treatment, 6 to 8 weeks after treatment, and 7 to 8 months after treatment. Results At baseline, 29 eyes (29 patients) were included both in the PDT and in the HSML group. The mean (± standard deviation) CVI change in the HSML group between before PDT and 6 to 8 weeks after PDT was ? 0.009 ± 0.032 (p = 0.127), whereas this was 0.0025 ± 0.037 (p = 0.723) between the visit before PDT and final visit. The patients in the PDT group had a CVI change of ? 0.0025 ± 0.037 (p = 0.723) between the visit before PDT and first visit after PDT, and a mean CVI change of ? 0.013 ± 0.038 (p = 0.080) between the visit before PDT and final visit. There was no significant correlation between CVI and BCVA at the measured time points, in both the HSML group (p = 0.885), and in the PDT group (p = 0.904). Moreover, no significant changes in CVI occurred in the unaffected eye at any time point. Conclusions PDT and HSML do not significantly affect CVI, and therefore a CVI change may not be primarily responsible for the treatment effect. The positive treatment effect of both interventions may rely on other mechanisms, such as an effect on choriocapillaris and/or retinal pigment epithelium function.
关键词： Choroidal vascularity index,Photodynamic therapy,Central serous chorioretinopathy,Micropulse laser
更新于2025-09-23 15:19:57
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Light-Triggered Dual-Modality Drug Release of Self-Assembled Prodrug-Nanoparticles for Synergistic Photodynamic and Hypoxia-Activated Therapy
摘要： Photodynamic therapy (PDT) leads to tumor hypoxia which could be utilized for the activation of hypoxia-activated prodrugs (HAPs). However, conventional photosensitizer-loaded nanoformulations suffer from aggregation-caused quenching (ACQ) effect, which limits the efficiency of PDT and synergistic therapy. Herein, prodrug-nanoparticles (NPs) are prepared by the self-assembly of heterodimeric prodrugs composed of pyropheophorbide a (PPa), hypoxia-activated prodrug PR104A, and a thioether or thioketal linkage. In addition, a novel dual-modality drug release pattern is proposed on the basis of the structural states of prodrug-NPs. Under light irradiation, PR104A is released via photoinduced electron transfer (PET) due to the aggregating state of prodrugs. With the disassembly of prodrug-NPs, ACQ effect relieves, PPa produces singlet oxygen which further promotes the reactive oxygen species (ROS)-sensitive release of PR104A. Such prodrug-NPs turn the disadvantage of the ACQ effect to facilitate drug release, demonstrating high-efficiency synergy in combination with PDT and hypoxia-activated therapy.
关键词： aggregation-caused quenching,Photodynamic therapy,drug release,photoinduced electron transfer,hypoxia-activated prodrugs,reactive oxygen species
更新于2025-09-23 15:19:57
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Antimicrobial photodynamic therapy with Bixa orellana extract and blue LED in the reduction of halitosisa??A randomized, controlled clinical trial
摘要： This study aimed to evaluate the reduction of halitosis when using antimicrobial photodynamic therapy (aPDT) with Bixa orellana extract and blue light-emitting diode (LED). Methods: Forty-four UNINOVE students or employees with a diagnosis of sulfide (H2S) ≥ 112 ppb in gas chromatography were selected. The patients were randomly divided in groups: Group 1 (n=15): aPDT with annatto and LED; Group 2 (n=14): tongue scraping; Group 3 (n=15): tongue scraping and aPDT. For aPDT, a was Bixa orellana extract used in a concentration of 20% w/v (Fórmula e A??o?, S?o Paulo, Brazil) on the tongue for 2 minutes, associated with a blue-violet LED (Valo Cordless Ultradent? Products, Inc., South Jordan, UT, USA) (395-480 nm). Six points were irradiated on the back of the tongue, at wavelength 395-480 nm for 20 seconds, energy of 9.6 J and radiant energy of 6.37 J/cm2 per point. The results were compared before, immediately after treatment and 7 days after. The Friedman test was used for the intragroup analysis and the Kruskal Wallis test for the intergroup analysis. Results: In all groups, there was a difference between baseline and the value immediately after the treatment. In Groups 1 and 3, there was no difference between the baseline and the 7 days control. Conclusion: There was an immediate reduction of halitosis, but the reduction was not maintained after 7days.
关键词： Light emitting diode,Photodynamic therapy,Bixa orellana,Halitosis
更新于2025-09-23 15:19:57
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Self-Assembled Nanophotosensitizing Systems with Zinc(II) Phthalocyanine-Peptide Conjugates as Building Blocks for Targeted Chemo-Photodynamic Therapy
摘要： Two zinc(II) phthalocyanines (ZnPcs) substituted with a short peptide (Gly-Gly-Lys) with either a carboxyl or a carbamoyl group at the C-terminus and an appended biotin moiety were prepared and characterized. They could self-assemble into spherical nanoparticles, namely ZnPc-GGK(B)-COOH NP and ZnPc-GGK(B)-CONH2 NP, through noncovalent interactions, which encapsulated the hydrophobic ZnPc units in the core and exposed the biotin moieties on the surface. The zeta potential of ZnPc-GGK(B)-COOH NP in water was found to be ?28 mV, whereas that of ZnPc-GGK(B)-CONH2 NP was in opposite sign (+15 mV), reflecting the different functionality at the C-terminus, which also greatly affected the stability of the self-assembled nanoparticles. The targeting effect of ZnPc-GGK(B)-COOH NP was examined against human hepatocellular carcinoma HepG2 cells, which overexpress biotin receptor, and Chinese Hamster Ovary CHO-K1 cells, which have a low expression of biotin receptor. This nanosystem was also coassembled with the chemotherapeutic doxorubicin (DOX) to form ZnPc-GGK(B)-COOH/DOX NP. Both ZnPc-GGK(B)-COOH NP and ZnPc-GGK(B)-COOH/DOX NP could induce photocytotoxicity and apoptosis on HepG2 cells with an IC50 value of 1.48 and 0.49 μM ZnPc, respectively. For the latter nanosystem, the ZnPc and DOX components induced cytotoxicity in a synergistic manner. The photodynamic and chemotherapeutic effects of these two nanosystems were also examined on nude mice bearing a human colorectal adenocarcinoma HT29 tumor. The ZnPc-GGK(B)-COOH/DOX NP exhibited a stronger tumor inhibition effect upon irradiation, demonstrating the presence of dual chemo-photodynamic therapeutic actions.
关键词： photodynamic therapy,phthalocyanine,peptide,nanomedicine,self-assembly
更新于2025-09-23 15:19:57
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Optically controlled hybrid metamaterial of plasmonic spiky gold inbuilt graphene sheets for bimodal imaging guided multimodal therapy
摘要： The development of multifunctional molecular diagnostic platforms for concordant visualization and treatment of diseases with high sensitivity and resolution has recently become a crucial strategy in cancer management. Thus, engineering functional metamaterials with high therapeutic and imaging capabilities to elucidate diseases from morphological behaviors to physiological mechanisms is an unmet need in the current scenario. Here, we report the design of a unique hybrid plasmonic nanoarchitecture, for targeted multiple photo-therapies of breast cancer by simultaneous real time monitoring through fluorescence and surface enhanced Raman scattering (SERS) techniques. The nanoframework consists of plasmonic gold-graphene hybrids tethered with folic acid ligated chitosan modified photosensitizer (PpIX) to afford target specific localized photothermal and photodynamic therapy. The hybrid vehicle also served as an excellent nanocarrier for efficient loading and stimuli responsive release of the chemotherapeutic drug doxorubicin (DOX) to enhance the therapeutic efficacy, thereby forming a trimodal nanomedicine against cancer. The cytotoxic effects induced by the cumulative action of triplet therapeutic tools were visualized through both fluorescence and SERS imaging channels. Moreover, it also generated synchronized therapeutic effects resulting in the effective regression of tumor volume without propagating any toxic effect to other organs of the animals. Taken together, by virtue of strong light-matter interactions, our nanoprobe showed enhanced photoadsorption which facilitate the amplified light reactive therapeutic and imaging efficacies along with targeted and enhanced chemotherapy, both in vitro and in vivo, which may offer a promising outcome in clinical research.
关键词： surface enhanced Raman scattering (SERS),hybrid plasmonic nanoarchitecture,breast cancer,trimodal nanomedicine,fluorescence,doxorubicin (DOX),photothermal and photodynamic therapy,multifunctional molecular diagnostic platforms
更新于2025-09-23 15:19:57
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Glutathione- and light-controlled generation of singlet oxygen for triggering drug release in mesoporous silica nanoparticles
摘要： A combined stimuli-responsive photosensitiser and drug release system based on mesoporous silica nanoparticles was prepared. This nanoplatform encapsulated molecules of a zinc(II) phthalocyanine substituted with a glutathione-cleavable 2,4-dinitrobenzenesulfonate quencher and doxorubicin linked via a singlet-oxygen-cleavable 9,10-dialkoxyanthracene linker. In the presence of glutathione (in mM range) and upon irradiation (λ > 610 nm), the phthalocyanine units were activated by detaching from the quenching component to emit fluorescence and generate singlet oxygen. The latter subsequently cleaved the 9,10-dialkoxyanthracene linker to trigger the release of a doxorubicin derivative. The glutathione- and light-controlled activation and drug-release processes on this nanoplatform were demonstrated in phosphate buffered saline. The activation in fluorescence emission by intracellular thiols was also shown inside HepG2 human hepatocellular carcinoma cells. Upon irradiation, the nanosystem exhibited high cytotoxicity due to the photodynamic effect of the activated phthalocyanine units, but the cytotoxic effect of the released Dox moieties was not notable probably due to their reduced cytotoxicity as a result of the pendant substituent and the low drug loading in the nanoparticles.
关键词： mesoporous silica nanoparticles,photosensitiser,photodynamic therapy,doxorubicin,drug release,glutathione,singlet oxygen
更新于2025-09-23 15:19:57
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Nucleus-targeted organoiridium-albumin conjugate for photoactivated cancer therapy
摘要： A novel organoiridium-albumin bioconjugate (Ir1-HSA) was synthesized via reaction of a pendant maleimide ligand with human serum albumin. The phosphorescence of Ir1-HSA was enhanced significantly compared to the parent complex Ir1. The long phosphorescence lifetime and high 1O2 quantum yield of Ir1-HSA are highly favourable properties for photodynamic therapy. Ir1-HSA mainly accumulated in the nucleus of living cancer cells and showed remarkable photocytotoxicity against a range of cancer cell lines and tumor spheroids (light IC50; 0.8-5 μM, photo-cytotoxicity index PI = 40-60) while remaining non-toxic to normal cells and normal cell spheroids, even after photo-irradiation. This nucleus-targeting organoiridium-albumin is a strong candidate photosensitizer for anticancer photodynamic therapy.
关键词： Photodynamic therapy,Photosensitizer,Albumin,Organoiridium
更新于2025-09-23 15:19:57
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A Conservative Approach for Localized Spongiotic Gingivitis Hyperplasia Using Photodynamic Therapy: A Case Report and Review of the Literature
摘要： Objective: To describe a clinical case of successful conservative management of Localized Juvenile Spongiotic Gingivitis Hyperplasia (LJSGH) using photodynamic therapy (PDT) and reviews the current literature on this pathology. Background data: LJSGH is a recently described rare disease with controversial treatment results. As of today, 13 publications report surgical treatment approaches. The use of CO2 laser and cryotherapy was reported only in one study. The use of PDT was not previously reported. Patients and methods: A 9-year-old male patient was referred to our institution with the chief complaint of asymptomatic "inflamed gingiva" starting 1 year before. Clinical examination revealed an erythematous line accompanying the gingival contour, with a certain degree of hyperplasia. The diagnosis of LJSGH was performed based on clinical features and later confirmed histopathologically. A novel approach using PDT was then proposed. The photosensitizer was methylene blue, and a semiconductor laser diode was used. Results: One week after starting PDT, gingival hyperplasia was partially reduced. Immediately after the end of treatment, a significant reduction of gingival hyperplasia was observed. PDT proved to be safe, quick and painless, with no esthetic harm. Conclusions: This case illustrates the benefit of a more conservative approach as opposed to surgical procedure, with good clinical response and decreased morbidity over a 2-year follow-up period.
关键词： photodynamic therapy,low-level laser therapy,Juvenile Spongiotic Gingivitis Hyperplasia
更新于2025-09-19 17:15:36