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Proof of concept of plasmonic thermal destruction of surface cancers by gold nanoparticles obtained by green chemistry
摘要： A greener approach for the design of surface plasmon resonant gold nanoparticles has been obtained with a hydrosoluble fraction of an endemic asteraceae medicinal plant. This medicinal plant is originated from Indian Ocean and demonstrates its bioreducing activity in the design of stable green nanomedicine in aqueous media. This article describes the preclinical assessment of the efficacy of these novel nanocandidates on murine model by intratumoral and intravenous injections. It definitely demonstrates two key points in the treatment of cancer: 1) optimization of the tumor microenvironment targeting by specific ligands for a limited damage on healthy tissue, 2) the need to screen the specific irradiation dose (time, power) taking into account the type of tumor.
关键词： Medicinal plant,Hyperthermia,Green nanomedicine,Plasmonic photothermal therapy,Gold nanoparticle
更新于2025-11-14 15:25:21
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Intercellular Trafficking of Gold Nanostars in Uveal Melanoma Cells for Plasmonic Photothermal Therapy
摘要： Efficient plasmonic photothermal therapies (PPTTs) using non-harmful pulse laser irradiation at the near-infrared (NIR) are a highly sought goal in nanomedicine. These therapies rely on the use of plasmonic nanostructures to kill cancer cells while minimizing the applied laser power density. Cancer cells have an unsettled capacity to uptake, retain, release, and re-uptake gold nanoparticles, thus offering enormous versatility for research. In this work, we have studied such cell capabilities for nanoparticle trafficking and its impact on the effect of photothermal treatments. As our model system, we chose uveal (eye) melanoma cells, since laser-assisted eye surgery is routinely used to treat glaucoma and cataracts, or vision correction in refractive surgery. As nanostructure, we selected gold nanostars (Au NSs) due to their high photothermal efficiency at the near-infrared (NIR) region of the electromagnetic spectrum. We first investigated the photothermal effect on the basis of the dilution of Au NSs induced by cell division. Using this approach, we obtained high PPTT efficiency after several cell division cycles at an initial low Au NS concentration (pM regime). Subsequently, we evaluated the photothermal effect on account of cell division upon mixing Au NS-loaded and non-loaded cells. Upon such mixing, we observed trafficking of Au NSs between loaded and non-loaded cells, thus achieving effective PPTT after several division cycles under low irradiation conditions (below the maximum permissible exposure threshold of skin). Our study reveals the ability of uveal melanoma cells to release and re-uptake Au NSs that maintain their plasmonic photothermal properties throughout several cell division cycles and re-uptake. This approach may be readily extrapolated to real tissue and even to treat in situ the eye tumor itself. We believe that our method can potentially be used as co-therapy to disperse plasmonic gold nanostructures across affected tissues, thus increasing the effectiveness of classic PPTT.
关键词： nanoparticle endocytosis,femtosecond pulse laser,nanoparticle exocytosis,gold nanostars,plasmonic photothermal therapy
更新于2025-09-23 15:21:01
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Single-Cell ATP Content Monitoring during Hyperthermia Cell Death by using Plasmonic Fluorescent Nanoflare
摘要： Gold nanorods-based plasmonic photothermal therapy (AuNRs-PPTT) has been a prospective anti-cancer approach in which AuNRs absorbs near-infrared (NIR) light and converts it into heat, leading to cell death. Investigating molecular energy metabolism of single cells, especially cancer cells, during hyperthermia cell death process is therefore of great significance, as it can help us to better understand the photothermal lethal mechanism of cancer cells and design new photothermal probes more rationally. However, during the AuNRs-PPTT process, how the cells respond to heat stimulation, and how their energy metabolism changes, these basic issues have rarely been studied. Herein, we selected adenosine triphosphate (ATP) as a target molecule, and by preparing a plasmonic and turn-on type fluorescent nanoprobe we examined the ATP metabolism difference between cancerous cells and normal cells during the AuNRs-PPTT process. We found that the fluorescence intensity increased ~ 60% after 5 min laser irradiation as compared to the initial intensity in single HeLa cells, but only ~ 20% increasement was observed for single H8 cells; obviously the increase of ATP content in cancerous cells was notably higher than that in normal cells during the hyperthermia cell death.
关键词： Plasmonic photothermal therapy,Fluorescent nanoprobe,ATP,Gold nanorods,Hyperthermia cell death
更新于2025-09-19 17:13:59