- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
<p>Gene Expression Profiling of the Liver and Lung in Mice After Exposure to ZnO Quantum Dots</p>
摘要： Introduction: ZnO quantum dots (QDs) have drawn much attention recently as they are Cd-free, low-cost, and have excellent optical properties. With the expanded production and application of ZnO nanoparticles, concerns about their potential toxicity have also been raised. Materials and Methods: We used RNA sequencing (RNA-seq) to analyze the global gene expression of liver and lung tissues after ZnO QDs treatment. Differentially expressed genes (DEGs) were screened, with a fold change >1.5 and padj <0.05. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed, and padj <0.05 was considered significantly enriched. The RNA-seq results were validated by quantitative real-time polymerase chain reaction (qRT-PCR). Results: A total of 47 and 218 genes were significantly differentially expressed in the liver and lung. Eight GO terms were enriched in the liver and lung, and retinol metabolism and the peroxisome proliferator-activated receptor (PPAR) signaling pathway were shared in different tissues. Discussion: According to DEGs and pathway enrichment analyses, inflammation might be induced in liver and lung tissues after intravenous injection of ZnO QDs. These findings will be helpful for future research and application of ZnO QDs.
关键词： ZnO QDs,transcriptome,differentially expressed genes
更新于2025-09-23 15:21:01
-
Vitamin B6 cofactors guided highly selective fluorescent turn-on sensing of histamine using beta-cyclodextrin stabilized ZnO quantum dots
摘要： Histamine, one of the most important biogenic amines (BAs) is considered as food hazard and therefore various agencies have fixed threshold in different food and beverages. In this manuscript, two novel fluorescent turn-on probes were developed for the instantaneous detection of histamine. The β-cyclodextrin (β-CD) capped ZnO quantum dots (QDs) were decorated with the vitamin B6 cofactors like pyridoxal 5′-phosphate (PLP) and pyridoxal (Py) by forming host-guest inclusion complexation between the capped β-CD and PLP/Py. The cofactors decorated QDs (ZnO@PLP and ZnO@Py) were applied for the sensing of BAs. Addition of histamine to the ZnO@PLP and ZnO@Py solution resulted selective fluorescence enhancement at 473 nm and 460 nm, respectively. Without any interference from the other tested BAs, the fluorescence response of the probes ZnO@PLP and ZnO@Py showed good linearity to histidine concentration from 2.49 to 24.4 μM and 7.44 to 47.6 μM with the detection limit down to 0.59 μM and 0.97 μM, respectively.
关键词： Vitamin B6 cofactors,ZnO QDs,Fluorescent turn-on sensor,Histamine
更新于2025-09-19 17:13:59
-
Water-soluble ZnO quantum dots modified by (3-aminopropyl)triethoxysilane: The promising fluorescent probe for the selective detection of Cu2+ ion in drinking water
摘要： Copper, as an essential element in human body, can have adverse impact on environment and healthy individuals if it is excessive. So it is necessary to establish a rapid and effective method for detecting Cu2+. In this work, we describe a method for determination of Cu2+ based on water-soluble ZnO quantum dots (QDs) modified with (3-aminopropyl)triethoxysilane (APTEs). The ZnO QDs functionalized with APTEs (NH2-ZnO QDs) synthesized by a simple sol-gel method and displayed strong yellow-green fluorescence with a peak at 535 nm under 350 nm excitation. High-resolution transmission electron microscopy, Fourier transform infrared spectroscopy, luminescence, and UV-visible absorption spectroscopy were used to characterize the NH2-ZnO QDs. In addition, the emission from NH2-ZnO QDs was selectively quenched upon addition of Cu2+. Therefore, this finding was used to design a fluorescent probe based on NH2-ZnO QDs to detect Cu2+ in water solution, and the linear relationships were 2-20 nM and 1-100 μM respectively, with detection limit for Cu2+ at 1.72 nM (on the basis of 3σ/slope criterion). This fluorescent probe had also been applied in real water sample to testify its availability in drinking water. Furthermore, the quenching mechanism was studied by measurements of UV-visible absorption spectra and fluorescent lifetime of ZnO QDs, which may be attributed to the aggregation induced by Cu2+ and the dynamic quenching existing energy transfer between QDs and Cu2+.
关键词： Drinking water,Water-soluble,Fluorescent probe,Cu2+ detection,ZnO QDs,Quenching mechanism
更新于2025-09-16 10:30:52
-
Concentration dependent thermo-optic properties of yellow emissive ZnO quantum dots
摘要： This paper study the thermal properties of ZnO quantum dots (QDs) synthesized by sol-gel method. The structural and morphological properties were investigated using x-ray diffraction technique (XRD) and transmission electron microscopy (TEM). Thermal properties of ZnO QDs were measured using dual beam mode matched thermal lens technique. The results reveal that the thermal diffusivity of the nano?uid depends on the concentration of ZnO QDs. There is an optimum concentration of ZnO QDs, above which thermal diffusivity increases more than that of the base ?uid whereas thermal diffusivity decreases below the optimum concentration. This variation in thermal diffusivity of ZnO QDs nano?uid is explained using absorption and emission spectra.
关键词： optical properties,ZnO QDs,thermal properties,nano?uids
更新于2025-09-11 14:15:04
-
ZnO Quantum Dots Modified by pH-Activated Charge-Reversal Polymer for Tumor Targeted Drug Delivery
摘要： In this paper, we reported a pH responsive nano drug delivery system (NDDS) based on ZnO quantum dots (QDs) for controlled release of drugs. Zwitterionic poly(carboxybetaine methacrylate) (PCBMA) and poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA) were introduced to modify ZnO QDs, which can help enhance water stability, increase blood circulation time, and promote endocytosis. After tuning of PCBMA/PDMAEMA ratios, the ZnO@P(CBMA-co-DMAEMA) nanoplatform shows a sensitive switch from strong protein adsorption resistance (with negatively charged surface) at physiological pH to strong adhesion to tumor cell membranes (with positively charged surface) at the slightly acidic extracellular pH of tumors. Anti-cancer drug, Doxorubicin (DOX), molecules were demonstrated to be successfully loaded to ZnO@P(CBMA-co-DMAEMA) with a relatively large drug loading content (24.6%). In addition, ZnO@P(CBMA-co-DMAEMA) loaded with DOX can achieve lysosomal acid degradation and release of DOX after endocytosis by tumor cells, resulting in synergistic treatment of cancer, which is attributed to a combination of the anticancer effect of Zn2+ and DOX.
关键词： Zinc oxide quantum dots (ZnO QDs),poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA),drug delivery,poly(carboxybetaine methacrylate) (PCBMA)
更新于2025-09-10 09:29:36