- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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Robust Photodynamic Therapy Using 5-ALA-Incorporated Nanocomplexes Cures Metastatic Melanoma through Priming of CD4 <sup>+</sup> CD8 <sup>+</sup> Double Positive T Cells
摘要： Advanced melanoma can rarely be cured. Photodynamic therapy (PDT) readily eradicates the primary melanoma but has limited ability to destroy the spreading tumor cells unless supported by other combinative interventions to augment systemic antitumor immunity. Based on the previously synthesized penetration-enhancing biomaterials, a topically administered nanoformulation is developed, which profoundly assists 5-aminolevulinic acid (5-ALA) in circumventing skin barrier to be selectively delivered to tumor cells. After endocytosis, accumulated 5-ALA is efficiently metabolized to a photosensitizer protoporphyrin IX (PpIX) which stimulates a large production of cytotoxic reactive oxygen species (ROS) under illumination. Accompanied by the robust inflammatory responses followed by primary tumor destruction, CD4+CD8+ double positive T cells are highly boosted to harness host immunity to purge metastases in lymphoid organs. Compared with dacarbazine and programmed death 1 (PD-1) antibody, this treatment in advanced melanoma murine models, achieves a striking curable rate of 90% without melanoma prognostic markers LDH and S-100B detection, followed by a relapse-free survival rate of 83.33% in 300 days. Moreover, the cured mice’s immune system function recovers to an extent similar to healthy mice without prolonged or exaggerated inflammation. This study using the synergistic biomaterials approach may thus render 5-ALA-mediated PDT a potentially curative therapy for advanced melanoma in clinic.
关键词： advanced melanoma,CD4+CD8+ double positive cells,relapse-free survival,5-aminolevulinic acid,cure
更新于2025-11-25 10:30:42
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A ratiometric fluorescence probe based on a novel recognition mechanism for monitoring endogenous hypochlorite in living cells
摘要： A ratiometric fluorescence probe (named ZOC) for the fast detection of HClO/ClO- was constructed by coumarin (donor) and pyridinium (acceptor) based on Forster resonance energy transfer (FRET) and intramolecular charge transfer (ICT) platform. ZOC possessed red emission signal (610 nm), large Stocks shift (190 nm), high energy transfer efficiency (95.3%), high selectivity and sensitivity, low detection limit (25 nM), wider detection range (from 25 nM to 30 μM), rapid response (within 13 S), and good biocompatibility. It was very interesting that the recognition mechanism involved a new organic reaction in which olefin double bond reacted first with HClO/ClO- regioselectively, followed by cyclization. ZOC was successfully used to the real time detection of endogenous HClO/ClO- in RAW 264.7 cells.
关键词： Hypochlorous acid/hypochlorite,Probe,Cell image,Ratiometric fluorescence,New mechanism
更新于2025-11-21 11:08:12
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On-off-on relay fluorescence recognition of ferric and fluoride ions based on indicator displacement in living cells
摘要： A new boronic acid derivative functionalized with a 4-(3-(4-(4,5-diphenyl-1H-imidazol-2-yl)phenyl)-1,2,4-oxadiazol-5-yl)phenyl (IOP) moiety was synthesized for use as a sequential “on-off-on”-type relay fluorescence probe for Fe3+ ions and F? ions with high selectivity and sensitivity under physiological conditions. The introduction of Fe3+ to IOP boronic acid (IOPBA) formed an Fe3+-IOPBA complex, which led to quenching of the blue fluorescence intensity at 458 nm. The lowest-energy conformation of IOPBA was theoretically predicted to adopt an extended structure, and the Fe3+ ion in the Fe3+-IOPBA complex was coordinated to two phenyl groups to form a p-complex. Upon addition of F? to the Fe3+-IOPBA complex, the original fluorescence was recovered due to formation of [FeF6]3?, resulting in “on-off-on”-type sensor behavior. IOPBA showed high selectivity towards Fe3+ among other cations. Moreover, the Fe3+-IOPBA complex showed specific selectivity towards F?, with other cations and anions not interfering with detection. Both sensing processes showed 1:1 stoichiometry with binding constants of 6.87 × 106 and 4.49 × 106 mol–1 L for Fe3+ with IOPBA and F? with Fe3+-IOPBA, respectively. The limits of detection for Fe3+ and F? were 10 and 1 nM, respectively. The proposed method was successfully applied in real water samples. Furthermore, the probe had low cytotoxicity and was successfully used as a bioimaging reagent to detect intracellular Fe3+ and F? in living HeLa cells.
关键词： Fluorescence imaging,On-off-on sensor,Probe for Fe3+ ions and F? ions,Living HeLa cells,Boronic acid derivative
更新于2025-11-21 11:08:12
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A new FRET-based ratiometric fluorescence probe for hypochlorous acid and its imaging in living cells
摘要： A novel ratiometric fluorescence probe for hypochlorous acid was constructed by coumarin and pyridinium fluorophore based on the Forster resonance energy transfer (FRET) and intramolecular charge transfer (ICT) platform. In this ICT/FRET system, the energy transfer efficiency is high to 94.3%. Moreover, the probe could respond to hypochlorous acid with high selectivity and sensitivity, and exhibited a large Stokes shift. It was interesting to find that the probe could recognize hypochlorous acid via a new mechanism, in which the a -position of carbonyl group was oxidized to form a diketone derivative. More importantly, the probe was successfully applied to the ratiometric imaging of both exogenous and endogenous hypochlorous acid in living RAW 264.7 cells, with low toxicity and high photo-stability.
关键词： New reaction mechanism,FRET,Hypochlorous acid,Fluorescence probe,Cell image,Ratiometric
更新于2025-11-21 11:08:12
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Aminoboronic acid-functionalized graphitic carbon nitride quantum dots for the photoluminescence multi-chemical sensing probe
摘要： This paper reports a highly sensitive photoluminescence glucose sensor based on aminoboronic acid-functionalized carbon nitride quantum dots (g-CNQDs/3APBA) fabricated using melamine and 3-aminophenylboronic acid via a facile two-step synthesis process. By introducing the covalent bonds between g-CNQDs and boronic acid groups, it can be effectively used as “on-off-on” based multi-chemical sensor. The g-CNQDs/3APBA exhibited quantum yields (QYs) as high as 78.5%, which is the highest QYs among fluorescence sensors based on g-CNQDs reported thus far. The material showed a wide linear range of 0 – 10 mM and a detection limit as low as 42 nM with excellent selectivity. In addition, it exhibited comparable performance compared to those of a commercial glucometer in a real blood test. Owing to the excellent bio-imaging properties and low cytotoxicity, g-CNQDs/3APBA is a promising candidate as a sensing material for biomedical and clinical applications.
关键词： quantum dots,glucose sensor,multi-chemical sensor,fluorescence,Graphitic carbon nitride,3-aminophenylboronic acid
更新于2025-11-20 15:33:11
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Facile ultrasonic synthesized NH2-carbon quantum dots for ultrasensitive Co2+ ion detection and cell imaging
摘要： The amine decorated carbon quantum dots (NH2-CQDs) were synthesized through ultrasonic method from graphite rods derived CQDs and ammonia hydroxide and utilized as the sensing probes for cobalt (II) ions and nucleic acids. The sensing technique was investigated to be the fluorescence quenching effect, which demonstrated linear relationship between cobalt (II) ions concentration and the emission intensity deviation ratio in the concentration range of 50 nM to 40 μM with the detection limit of 12 nM. In brief, this sensitive and selective detection method was confirmed to demonstrate high potential in cobalt (II) ions detection in real samples and nucleic acid sensing in biological cells.
关键词： Nucleic acid sensing,Carbon quantum dots (CQDs),Cobalt sensor
更新于2025-11-19 16:56:42
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Tailoring optical cross sections of gold nanorods at a target plasmonic resonance wavelength using bromosalicylic acid
摘要： In many applications, the optical cross sections of gold nanorods (AuNRs) are required to be tailored at a fixed target longitudinal surface plasmon resonance (LSPR) wavelength depending on the excitation source and the photodetector. In this work, we demonstrate the fine tailoring of optical cross sections of AuNRs at a fixed target resonance wavelength, on the basis of AuNR overgrowth using a binary surfactant mixture consisting of 5-bromosalicylic acid (BSA) and cetyltrimethylammonium bromide (CTAB). A systematic study was performed on the sum effects of the BSA concentration and the volume of the growth solution, which gives a formula for quantitative instructions. Based on the formula, we gave examples for the successful synthesis of AuNRs with different optical cross sections at target LSPR wavelengths. From simulation, a nonlinear relationship was further derived to understand the relationship between the aspect ratio and the width of the AuNRs at a target LSPR wavelength for the dimension design of AuNRs. The ratio of optical against physical cross sections was calculated and plotted as a function of the width. The results clearly indicate that AuNRs with a width of 30 nm possess the highest efficiency in terms of optical per physical cross section. Our study provides reliable methods for the synthesis, as well as guidelines for the dimension design of AuNRs, for use in a variety of applications.
关键词： 5-bromosalicylic acid,gold nanorods,optical cross sections,cetyltrimethylammonium bromide,surface plasmon resonance
更新于2025-11-19 16:56:42
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Influence of pretreatment on surface interaction between Cu and anatase-TiO2 in the simultaneous photoremediation of nitrate and oxalic acid
摘要： Copper-promoted anatase-type TiO2 photocatalysts (2.5 wt% Cu) were prepared by wet impregnation onto TiO2 which was pre-calcined at 600°C and the other not subjected to any thermal pre-treatment. In the latter case, the material was inactive for the photo-reduction of nitrate whereas 600°C pre-calcined TiO2 yielded a material which was active for the same reaction. The surface properties of the materials were determined by BET Surface area, SEM TEM, XRD, XPS, TPR, UV-Visible diffuse reflectance, DTA, N2O pulsed chemisorption and FTIR studies. The BET and XRD and DTA showed that pre-calcination of TiO2 stabilised the support, but coalescence of particles was observed in TiO2 that was not subjected to any thermal pre-treatment as evidenced by crystallite growth. Similarly, XPS, FTIR and TPR proved the formation of Cu2O particles on the surface of pre-calcined TiO2. On the other hand, the absence of pre-calcination step resulted in interring of Cu species within the grown anatase crystallites that hindered their proper distribution over TiO2, helped in its inactiveness in the photoreduction of nitrate. However, the prepared material using pre-calcined TiO2 showed the overall nitrate and oxalic acid removal efficiency of 31 and 70% with N2 and NH4+ selectivity of 44.9 and 55.1 %, respectively. The results provide insight into the significance of activity-structure relation, inferring that the two surfaces were chemically not similar. Thus, as even supported by adsorption experiment, difference in photocatalytic behaviour amongst the prepared materials was a function of crystallinity, particle size, absence of surface defect and high energy sites.
关键词： Nitrate,photocatalyst,photoremediation,metal oxide-support interaction,oxalic acid
更新于2025-11-19 16:56:35
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Synthesis, Crystal Structure, and Photocatalytic Properties of a Zinc(II) Coordination Polymer Based on 3-Hydroxy-2-pyridinecarboxylic Acid
摘要： The zinc(II) compound, [Zn3(HL)6]n (1) (H2L = 3-hydroxypyridine-2-carboxylic acid) was synthesized by a solvothermal reaction of Zn(NO3)2·6H2O and 3-hydroxypyridine-2-carboxylic acid as raw materials. The structure of complex 1 was determined by single-crystal X-ray diffraction analysis and further characterized by elemental analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis, as well as powder X-ray diffraction. X-ray structure analysis demonstrates that the complex crystallizes in the monoclinic system, space group P21/n. There are three zinc ions in the asymmetric unit, which are either five-coordinate or six-coordinate. The asymmetric units are further bridged by the carboxylate of the organic ligands, featuring a 2D framework. The solid state diffuse-reflectance UV/Vis spectra reveals that complex 1 has semiconducting nature with the energy bandgap (Eg) estimated to be 3.11 eV. The photocatalytic properties of complex 1 in degradation of organic dyes were further investigated. Results showed that the complex could degrade 54 % of the dye methylene blue solution within 120 min under UV irradiation light and reused for five times without the decline of the photocatalytic activity.
关键词： Crystal structure,Photocatalytic properties,Zinc,3-Hydroxypyridine-2-carboxylic acid,Solvothermal reaction,Coordination polymer
更新于2025-11-19 16:56:35
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An AND logic gate-based fluorescence probe for the detection of homovanillic acid, an indicator of the tumor
摘要： Homovanillic acid (HVA) is one of the major metabolites of catecholamine in human. An elevated level of HVA in urine and blood has been associated with many diseases, including catecholamine-secreting tumors. Consequently, detection of the biomarker HVA in urine is an effective strategy to detect cancer precursors and early stage cancers. In this study, a fluorescence probe is designed and fabricated by integrating lanthanide ions with metal-organic frameworks (MOFs). The fluorescence probe shows distinguished response toward HVA and exhibits ratiometric detection of HVA, rendering it an excellent candidate for sensing devices. Thus, we have designed logic gate operation integrating the probe with logical operations for the intelligent sensing of HVA. Furthermore, this type of luminesce based sensors integrated with logic gate augur for the further application in real-time early diagnosis of tumors in the future.
关键词： Rare earth,Homovanillic acid,Fluorescent probe,Logic gate,Ratiometric sensing,Metal-organic frameworks
更新于2025-11-19 16:56:35