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Efficient Exploration of the Composition Space in Ternary Organic Solar Cells by Combining High‐Throughput Material Libraries and Hyperspectral Imaging
摘要： Organic solar cells based on ternary active layers can lead to higher power conversion efficiencies than corresponding binaries, and improved stability. The parameter space for optimization of multicomponent systems is considerably more complex than that of binaries, due to both, a larger number of parameters (e.g., two relative compositions rather than one) and intricate morphology–property correlations. Most experimental reports to date reasonably limit themselves to a relatively narrow subset of compositions (e.g., the 1:1 donor/s:acceptor/s trajectory). This work advances a methodology that allows exploration of a large fraction of the ternary phase space employing only a few (<10) samples. Each sample is produced by a designed sequential deposition of the constituent inks, and results in compositions gradients with ≈5000 points/sample that cover about 15%–25% of the phase space. These effective ternary libraries are then colocally imaged by a combination of photovoltaic techniques (laser and white light photocurrent maps) and spectroscopic techniques (Raman, photoluminescence, absorption). The generality of the methodology is demonstrated by investigating three ternary systems, namely PBDB-T:ITIC:PC70BM, PTB7-Th:ITIC:PC70BM, and P3HT:O-IDFBR:O-IDTBR. Complex performance-structure landscapes through the ternary diagram as well as the emergence of several performance maxima are discovered.
关键词： high-throughput screening,layer-by-layer deposition,organic solar cells,ternaries,Raman imaging
更新于2025-09-12 10:27:22
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Beyond DPPH: Use of Fluorescence-Enabled Inhibited Autoxidation to Predict Oxidative Cell Death Rescue
摘要： Shah et al. develop a fluorescence-enabled inhibited autoxidation (FENIX) approach that permits accurate quantitation of radical-trapping antioxidant activity in phospholipid bilayers. The methodology is far superior to existing assays (e.g., DPPH) and enables reliable prediction of the anti-ferroptotic potency of redox-active compounds. The approach is easily amenable to high-throughput screening.
关键词： anti-ferroptotic potency,high-throughput screening,radical-trapping antioxidant activity,DPPH assay,phospholipid bilayers,FENIX
更新于2025-09-12 10:27:22
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High-throughput screening of laser additive manufactured metallic glass via ultrasonic wave
摘要： Laser additive manufacturing (LAM) technology provides an opportunity to fabricate bulk metallic glasses (BMGs) without any dimensional constraint and achieve the large-scale applications of BMGs. However, flaws, such as cracks, gas porosity, and crystalline phases, are always formed accompanied by the process of LAM, which seriously worsens the mechanical and physical properties of the resulting BMGs. Here, we present a novel method that involves ultrasonic wave technique to high-throughput screen the optimum process parameters for the LAM of BMG. A parameter library, constituted by a series of rectangular BMG samples, is rapidly fabricated by the LAM method under continuously changed combinations of laser power and travel speed. the ultrasonic attenuation factor, which is sensitive to the flaws, is used as the monitor to screen the parameters of the BMGs fabricated by the LAM. Using this approach, the laser power of 1300 W and travel speed of 600 mm/min are estimated as the optimum parameter combination for the LAM of a Zr51ti5ni10cu25Al9 (Zr51) BMG with the slightest flaws. The amorphous-phase dominated microstructure and the sufficiently high tensile strength of the subsequent fabricated large-sized Zr51 BMG sample verify this optimum parameter combination.
关键词： High-throughput screening,Bulk metallic glasses,Ultrasonic wave testing,Optimum process parameters,Laser additive manufacturing
更新于2025-09-11 14:15:04
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A simple turn-on fluorescent chemosensor for CO2 based on aggregation-induced emission: Application as a CO2 absorbent screening method
摘要： An a-cyanostilbene-based fluorescent chemosensor, which includes a primary amine, was developed for the quantitative analysis of CO2 in solution. (Z)-3-(4-(3-Aminopropoxy)phenyl)-2-(4-nitrophenyl)acrylonitrile (1) reacts with CO2 and enables a turn-on type CO2 chemosensor with increased fluorescence due to aggregation-induced emission. More importantly, 1 required no additives such as an amine media for detecting CO2, and the limit of detection was estimated to be ~26 ppm. This system was successfully applied to determine the relative efficiency of CO2 absorbents dependent on the fluorescence change in 1. The use of this chemosensor as a CO2 absorbent screening method suggests that 1 may be used to develop a high-throughput screening method for the evaluation of high-performance CO2 absorbents.
关键词： High throughput screening,Aggregation-induced emission,CO2 absorbent screening,Fluorescent chemosensor,CO2
更新于2025-09-10 09:29:36
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Computational screening of bulk materials with intrinsic intermediate band
摘要： Intermediate band compounds are promising high-efficiency materials for solar cells. Besides that, they also show unusual properties that make them interesting from a fundamental point of view. In this work, we create a series of filters capable of searching the AFLOW-ICSD materials database for compounds that show intrinsic intermediate bands. We analyse all available binary and ternary compounds, totaling 45,656 entries. The selected materials show at least one narrow band (maximum width of 1 eV) next to the energy gap. After removal of repeated entries and of wide bandgap insulators, we end up with a total of 660 materials that present intermediate bands. From this group, the majority has completely empty or filled intermediate bands or, during new calculations, changed to another state with a different type of intermediate band or no band of this type. Nevertheless, three compounds fulfill all criteria and continue to present a partially filled intermediate band (Bi2Rh2O7, Ca5FeN6, and OsTb6I10), showing potential for solar cells applications.
关键词： Intermediate band,Solar cells,Materials screening,High-throughput screening,DFT calculations
更新于2025-09-10 09:29:36
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A fluorescent 3D cell culture assay for high throughput screening of cancer drugs down-regulating survivin
摘要： Survivin, a member of inhibitor of apoptosis family, is currently undergoing intensive investigations as a promising cancer marker due to its overexpression in multiple tumor tissues and close relationship with chemotherapy resistance. In this study, a novel 3D survivin promoter assay was developed, using enhanced green fluorescent protein (EGFP) as the reporter to assess survivin promoter activity for cancer drug screening. Breast cancer MCF-7 cells were engineered to express EGFP controlled by a human survivin promoter and a CMV promoter, respectively. These cells were cultured in three-dimensional (3D) polymer-based scaffolds on a 40-microbioreactor platform (40-MBR) with real-time monitoring of EGFP signals. The EGFP production driven by the survivin promoter was strongly correlated with survivin transcriptional level in MCF-7 cells treated with YM155, a small-molecule survivin promoter suppressant. Moreover, the potential inhibition effects of doxorubicin and cisplatin on survivin and their cytotoxicity were also evaluated in this system. This study demonstrated the potential application of the novel 3D survivin promoter-EGFP reporter assay for high-throughput screening of chemicals down-regulating survivin as a molecular target for cancer therapy.
关键词： Cancer,drug discovery,survivin,high throughput screening,3D cell culture
更新于2025-09-10 09:29:36
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A polarization sensitive light scattering unit for high throughput screening
摘要： A simple, effective light scattering prototype sensitive to both polarized and depolarized scattering was constructed, and its performance was tested on a variety of pure liquids and optically isotropic and anisotropic polymer solutions and colloidal suspensions. The results, performance, and means to further improvement are reported here. Because of its simplicity and low cost, many identical units can be produced to construct a simultaneous multiple sample light scattering platform that can be used to monitor polymer and colloid solution stability, phase changes, aggregation, degradation, etc. Measurable depolarization was found for a variety of organic liquids and suspensions of both poly?uoroethylene and latex spheres. No detectable depolarization was found for various polymers.
关键词： light scattering,depolarized scattering,colloidal suspensions,polymer solutions,high throughput screening,polarization sensitive
更新于2025-09-10 09:29:36
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Automated Behavioral Analysis of Large <em>C. elegans</em> Populations Using a Wide Field-of-view Tracking Platform
摘要： Caenorhabditis elegans is a well-established animal model in biomedical research, widely employed in functional genomics and ageing studies. To assess the health and fitness of the animals under study, one typically relies on motility readouts, such as the measurement of the number of body bends or the speed of movement. These measurements usually involve manual counting, making it challenging to obtain good statistical significance, as time and labor constraints often limit the number of animals in each experiment to 25 or less. Since high statistical power is necessary to obtain reproducible results and limit false positive and negative results when weak phenotypic effects are investigated, efforts have recently been made to develop automated protocols focused on increasing the sensitivity of motility detection and multi-parametric behavioral profiling. In order to extend the limit of detection to the level needed to capture the small phenotypic changes that are often crucial in genetic studies and drug discovery, we describe here a technological development that enables the study of up to 5,000 individual animals simultaneously, increasing the statistical power of the measurements by about 1,000-fold compared to manual assays and about 100-fold compared to other available automated methods.
关键词： nematode library,neurodegeneration,amyloid formation,Alzheimer's disease,Drug discovery,Issue 141,phenotype-based screening,high-throughput screening,Immunology and Infection,C. elegans,large population analysis
更新于2025-09-09 09:28:46
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Recent Advances in Design of Fluorescence-based Assays for High-throughput Screening
摘要： Significant improvements in disease management have been achieved in the past few decades. Using cancer as an example, survival rates have increased dramatically in economically developed countries, according to the World Cancer Report published by WHO in 2014.1 One of the highlights is the average 5-year survival rate of children diagnosed before the age of 15 years in Britain increased from less than 30% in 1966-70 to almost 80% in 1996-2000. The improvement can be attributed to the establishment of early screening and awareness programs, identification of a large number of genetic or non-genetic risk factors or markers, and availability of diverse therapeutic strategies including surgery and treatments using radioactive reagents, small molecules, or biological drugs. Still, cancer remains one of the major causes of deaths worldwide, affecting millions of people. Survival rates in developing countries are poor, and the global and regional burden of cancer in terms of both incidence and mortality keep increasing along with the growing population and aging society. Further improvement heavily relies on early diagnosis, wide access to treatment options, and discovery of more effective biomarkers as well as anticancer drugs with high efficacy. Such demands are also applied to diagnosis and cure for all other diseases, calling for rapid advancements in high-throughput screening (HTS) technologies that can efficiently test a large number of targets and samples, monitor diverse enzymatic processes in situ, and effectively identify biologically active substances.2
关键词： Fluorescence-based assays,High-throughput screening,Disease diagnosis,Drug discovery,Biomarkers
更新于2025-09-09 09:28:46
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Zebrafish larvae show negative phototaxis to near-infrared light
摘要： Zebrafish larvae (Danio rerio) are among the most used model species to test biological effects of different substances in biomedical research, neuroscience and ecotoxicology. Most tests are based on changes in swimming activity of zebrafish larvae by using commercially available high-throughput screening systems. These systems record and analyse behaviour patterns using visible (VIS) and near-infrared (NIR) light sources, to simulate day (VIS) and night (NIR) phases, which allow continuous recording of the behaviour using a NIR sensitive camera. So far, however, the sensitivity of zebrafish larvae to NIR has never been tested experimentally, although being a critical piece of information for interpreting their behaviour under experimental conditions. Here, we investigated the swimming activity of 96 hpf (hours post fertilization) and 120 hpf zebrafish larvae under light sources of NIR at 860 nm and at 960 nm wavelength and under VIS light. A thermal source was simultaneously presented opposite to one of the light sources as control. We found that zebrafish larvae of both larval stages showed a clear negative phototactic response towards 860 nm NIR light and to VIS light, but not to 960 nm NIR light. Our results demonstrated that zebrafish larvae are able to perceive NIR at 860 nm, which is almost identical to the most commonly used light source in commercial screening systems (NIR at 850 nm) to create a dark environment. These tests, however, are not performed in the dark from the zebrafish′s point of view. We recommend testing sensitivity of the used test organism before assuming no interaction with the applied light source of commonly used biosensor test systems. Previous studies on biological effects of substances to zebrafish larvae should be interpreted with caution.
关键词： Zebrafish larvae,near-infrared light,high-throughput screening systems,negative phototaxis,behavioral response
更新于2025-09-09 09:28:46