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Ordered assemblies of Fe3O4 and a donor-acceptor-type π-conjugated polymer in nanoparticles for enhanced photoacoustic and magnetic effects
摘要： We report that the ordered structure in the assemblies of iron oxide nanoparticles in conjugated polymer nanoparticles is the key to achieve better properties to realize multimodal theranostic agents for magnetic resonance and photoacoustic imaging. Hybrid nanoparticles of a conjugated polymer (PCPDTBT), a phospholipid (D8PE) with a primary amine polar head, and iron oxide (Fe3O4) nanoparticles were prepared by a phase-separated film shattering process by varying the iron oxide concentration while maintaining a fixed mixing ratio of PCPDTBT and D8PE. Notably, the hybrid nanoparticles assembled at a molar mixing ratio of 1:1:0.8 (PCPDTBT/D8PE/Fe3O4) exhibited the shortest transversal relaxation time, T2, and a photoacoustic signal 22 times higher than that obtained at the 1:1:0 mixing ratio. Structural analysis by X-ray diffraction together with the measurements of energy transfer by transient absorption spectroscopy confirmed that the structural ordering of these hybrid nanoparticles was responsible for their enhanced photoacoustic and magnetic properties.
关键词： Conjugated polymers,Photoacoustic imaging,Magnetic resonance imaging,Iron oxides,Hybrid nanoparticles
更新于2025-09-23 15:22:29
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Fast Padé Transform for increasing the signal to noise ratio of spectra provided by STEAM pulse sequence
摘要： BACKGROUND/OBJECTIVE: There are two routine pulse-sequences for single voxel spectroscopy (SVS), point resolved spectroscopy (PRESS) and stimulated echo acquisition mode (STEAM). Although STEAM has several advantages in comparison to PRESS, signal/noise ratio (SNR) superiority of PRESS makes it the first choice for SVS. Application of Fast Padé transform (FPT) instead of Fast Furrier transform (FFT) might increase the SNR of the signal produced by STEAM pulse-sequence and therefore allows the benefits of its advantages. We aimed to evaluate and compare the noise root mean square (RMS) and SNR provided by STEAM pulse-sequence using both FPT and FFT. MATERIALS AND METHOD: A gelatin-based phantom was constructed in a 19-cm acrylic cylinder. The phantom had two normal/tumoral parts. The SVS was performed using a 3T MRI scanner. STEAM pulse-sequence were used with the following parameters: TR = 2000 ms, TM = 10 ms, and three TEs of 20, 135 and 270 ms with two data-points of 1024 and 512 and voxel-size of 1 cm3. The raw data were extracted and processed using both FFT and FPT estimators to produce the spectrum. The noise RMS and SNR of Cho and Cr metabolites were assessed. RESULTS: According to the results, noise RMS of spectra provided by FPT were decreased between 3619.01–14252.94% in comparison to FFT (p < 0.00001). The SNR of Cr1 and Cho peaks of the spectra provided by FPT were increased more than 96.80 and 97.18, respectively (0.00006 < p < 0.02). DISCUSSION: The difference of noise RMS’s provided by FPT are thousands percent less than FFT. This enormous decrease in noise provides a good increase of SNR. While the range of Cr1 and Cho SNR by FFT are between 41.55–120.32 the range of SNRs of these peaks provided by FPT are between 1719.99–9744.79, which implies a significant difference between the efficiency of FPT and FFT. CONCLUSION: This study showed that application of FPT in comparison to FFT can increase the spectra SNR and so that its usage can be helpful during the application of STEAM pulse-sequence which results in lower SNR in comparison to PRESS pulse-sequence. Thus, we should make use of the advantages of STEAM pulse-sequence.
关键词： Magnetic resonance spectroscopy,signal to noise ratio,STEAM pulse sequence,Fast Padé Transform,Fast Furrier Transform
更新于2025-09-23 15:22:29
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Diagnostic imaging and radiation exposure in inflammatory bowel disease
摘要： Diagnostic imaging plays a key role in the diagnosis and management of inflammatory bowel disease (IBD). However due to the relapsing nature of IBD, there is growing concern that IBD patients may be exposed to potentially harmful cumulative levels of ionising radiation in their lifetime, increasing malignant potential in a population already at risk. In this review we explore the proportion of IBD patients exposed to high cumulative radiation doses, the risk factors associated with higher radiation exposures, and we compare conventional diagnostic imaging with newer radiation-free imaging techniques used in the evaluation of patients with IBD. While computed tomography (CT) performs well as an imaging modality for IBD, the effective radiation dose is considerably higher than other abdominal imaging modalities. It is increasingly recognised that CT imaging remains responsible for the majority of diagnostic medical radiation to which IBD patients are exposed. Magnetic resonance imaging (MRI) and small intestine contrast enhanced ultrasonography (SICUS) have now emerged as suitable radiation-free alternatives to CT imaging, with comparable diagnostic accuracy. The routine use of MRI and SICUS for the clinical evaluation of patients with known or suspected small bowel Crohn’s disease is to be encouraged wherever possible. More provision is needed for out-of-hours radiation-free imaging modalities to reduce the need for CT.
关键词： Diagnostic medical radiation,Small intestine contrast-enhanced ultrasonography,Nuclear medicine,Computerised tomography,Inflammatory bowel disease,Magnetic resonance enterography,Small bowel follow-through
更新于2025-09-23 15:22:29
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Significance of Additional Non-Mass Enhancement in Patients with Breast Cancer on Preoperative 3T Dynamic Contrast Enhanced MRI of the Breast
摘要： Background: In preoperative assessment of breast cancer, MRI has been shown to identify more additional breast lesions than are detectable using conventional imaging techniques. The characterization of additional lesions is more important than detection for optimal surgical treatment. Additional breast lesions can be included in focus, mass, and non-mass enhancement (NME) on MRI. According to the fifth edition of the breast imaging reporting and data system (BI-RADS?), which includes several changes in the NME descriptors, few studies to date have evaluated NME in preoperative assessment of breast cancer. Objectives: We investigated the diagnostic accuracy of BI-RADS descriptors in predicting malignancy for additional NME lesions detected on preoperative 3T dynamic contrast enhanced MRI (DCE-MRI) in patients with newly diagnosed breast cancer. Patients and Methods: Between January 2008 and December 2012, 88 patients were enrolled in our study, all with NME lesions other than the index cancer on preoperative 3T DCE-MRI and all with accompanying histopathologic examination. The MRI findings were analyzed according to the BI-RADS MRI lexicon. We evaluated the size, distribution, internal enhancement pattern, and location of NME lesions relative to the index cancer (i.e., same quadrant, different quadrant, or contralateral breast). Results: On histopathologic analysis of the 88 NME lesions, 73 (83%) were malignant and 15 (17%) were benign. Lesion size did not differ significantly between malignant and benign lesions (P = 0.410). Malignancy was more frequent in linear (P = 0.005) and segmental (P = 0.011) distributions, and benignancy was more frequent in focal (P = 0.004) and regional (P < 0.001) NME lesions. The highest positive predictive value (PPV) for malignancy occurred in segmental (96.8%), linear (95.1%), clustered ring (100%), and clumped (92.0%) enhancement. Asymmetry demonstrated a high positive predictive value of 85.9%. The frequency of malignancy was higher for NME lesions located in the same quadrant with the index cancer (P = 0.006), and benignancy was higher in the contralateral breast (P = 0.015). On multivariate analysis, linear (P = 0.001) and segmental (P = 0.005) distributions were significant predictors of malignancy. Conclusion: The possibility of malignancy is strongly indicated when additional NME lesions show linear or segmental enhancement on preoperative 3T DCE-MRI in patients with recently diagnosed breast cancer.
关键词： Non-Mass Enhancement,Magnetic Resonance Imaging,Preoperative Care,Breast Neoplasms,Diagnosis
更新于2025-09-23 15:22:29
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Advanced Silicon Carbide Devices and Processing || Silicon Carbide for Novel Quantum Technology Devices
摘要： Silicon carbide (SiC) has recently been investigated as an alternative material to host deep optically active defects suitable for optical and spin quantum bits. This material presents a unique opportunity to realise more advanced quantum-based devices and sensors than currently possible. We will summarise key results revealing the role that defects have played in enabling optical and spin quantum measurements in this material such as single photon emission and optical spin control. The great advantage of SiC lies in its existing and well-developed device processing protocols and the possibilities to integrate these defects in a straightforward manner. There is particular current interest in nanomaterials and nanophotonics in SiC that could, once realised, introduce a new platform for quantum nanophotonics and in general for photonics. We will summarise SiC nanostructures exhibiting optical emission due to multiple polytypic bandgap engineering and deep defects. The combination of nanostructures and in-built paramagnetic defects in SiC could pave the way for future single-particle and single-defect quantum devices and related biomedical sensors with single-molecule sensitivity. We will review relevant classical devices in SiC (photonic crystal cavities, microdiscs) integrated with intrinsic defects. Finally, we will provide an outlook on future sensors that could arise from the integration of paramagnetic defects in SiC nanostructures and devices.
关键词： Optical-detected magnetic resonance,Single-photon sources,Silicon carbide deep defects,Paramagnetic properties
更新于2025-09-23 15:22:29
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A novel bioreactor for combined magnetic resonance spectroscopy and optical imaging of metabolism in 3D cell cultures
摘要： Purpose: Fluorescence lifetime imaging microscopy (FLIM) of endogenous fluorescent metabolites permits the measurement of cellular metabolism in cell, tissue and animal models. In parallel, magnetic resonance spectroscopy (MRS) of dynamic nuclear (hyper)polarized 13C‐pyruvate enables measurement of metabolism at larger in vivo scales. Presented here are the design and initial application of a bioreactor that connects these 2 metabolic imaging modalities in vitro, using 3D cell cultures. Methods: The model fitting for FLIM data analysis and the theory behind a model for the diffusion of pyruvate into a collagen gel are detailed. The device is MRI‐compatible, including an optical window, a temperature control system and an injection port for the introduction of contrast agents. Three‐dimensional printing, computer numerical control machining and laser cutting were used to fabricate custom parts. Results: Performance of the bioreactor is demonstrated for 4 T1 murine breast cancer cells under glucose deprivation. Mean nicotinamide adenine dinucleotide (NADH) fluorescence lifetimes were 10% longer and hyperpolarized 13C lactate:pyruvate (Lac:Pyr) ratios were 60% lower for glucose‐deprived 4 T1 cells compared to 4 T1 cells in normal medium. Looking at the individual components of the NADH fluorescent lifetime, τ1 (free NADH) showed no significant change, while τ2 (bound NADH) showed a significant increase, suggesting that the increase in mean lifetime was due to a change in bound NADH. Conclusion: A novel bioreactor that is compatible with, and can exploit the benefits of, both FLIM and 13C MRS in 3D cell cultures for studies of cell metabolism has been designed and applied.
关键词： multimodal,optical imaging,bioreactor,magnetic resonance spectroscopy (MRS),nicotinamide adenine dinucleotide (NADH),metabolism,fluorescence lifetime imaging (FLIM),lactate production
更新于2025-09-23 15:22:29
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Towards Real-time Metabolic Profiling of Cancer with Hyperpolarized Succinate
摘要： Purpose: The energy-yielding mitochondrial Krebs cycle has been shown in many cancers and other diseases to be inhibited or mutated. In most cells, the Krebs cycle with oxidative phosphorylation generates approximately 90% of the adenosine triphosphate in the cell. We designed and hyperpolarized carbon-13 labeled succinate (SUC) and its derivative diethyl succinate (DES) to interrogate the Krebs cycle in real-time in cancer animal models. Procedures: Using Parahydrogen Induced Polarization (PHIP), we generated hyperpolarized SUC and DES by hydrogenating their respective fumarate precursors. DES and SUC metabolism was studied in five cancer allograft animal models: breast (4T1), Renal Cell Carcinoma (RENCA), colon (CT26), lymphoma NSO, and lymphoma A20. Results: The extent of hyperpolarization was 8 ± 2% for SUC and 2.1 ± 0.6% for DES. The metabolism of DES and SUC in the Krebs cycle could be followed in animals 5 s after tail vein injection. The biodistribution of the compounds was observed using 13C FISP imaging. We observed significant differences in uptake and conversion of both compounds in different cell types both in vivo and in vitro. Conclusion: With hyperpolarized DES and SUC, we are able to meet many of the requirements for a useable in vivo metabolic imaging compound – high polarization, relatively long T1 values, low toxicity and high water solubility. However, succinate and its derivative DES are metabolized robustly by RENCA but not by the other cancer models. Our results underscore the heterogeneity of cancer cells and the role cellular uptake plays in hyperpolarized metabolic spectroscopy.
关键词： krebs cycle,hyperpolarization,metabolic imaging,succinate,magnetic resonance spectroscopy
更新于2025-09-23 15:22:29
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Weighted Manifold Alignment using Wave Kernel Signatures for Aligning Medical image Datasets
摘要： Manifold alignment (MA) is a technique to map many high-dimensional datasets to one shared low-dimensional space. Here we develop a pipeline for using MA to reconstruct high-resolution medical images. We present two key contributions. Firstly, we develop a novel MA scheme in which each high-dimensional dataset can be differently weighted preventing noisier or less informative data from corrupting the aligned embedding. We find that this generalisation improves performance in our experiments in both supervised and unsupervised MA problems. Secondly, we use the wave kernel signature as a graph descriptor for the unsupervised MA case finding that it significantly outperforms the current state-of-the-art methods and provides higher quality reconstructed magnetic resonance volumes than existing methods.
关键词： Slice stacking,Wave kernel signature,Magnetic resonance imaging,Manifold alignment,Graph descriptor
更新于2025-09-23 15:22:29
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Perspective: Prospects of non-invasive sensing of the human brain with diffuse optical imaging
摘要： Since the initial demonstration of near-infrared spectroscopy (NIRS) for noninvasive measurements of brain perfusion and metabolism in the 1970s, and its application to functional brain studies (fNIRS) in the 1990s, the field of noninvasive optical studies of the brain has been continuously growing. Technological developments, data analysis advances, and novel areas of application keep advancing the field. In this article, we provide a view of the state of the field of cerebral NIRS, starting with a brief historical introduction and a description of the information content of the NIRS signal. We argue that NIRS and fNIRS studies should always report data of both oxy- and deoxyhemoglobin concentrations in brain tissue, as they complement each other to provide more complete functional and physiological information, and may help identify different types of confounds. One significant challenge is the assessment of absolute tissue properties, be them optical or physiological, so that relative measurements account for the vast majority of NIRS and fNIRS applications. However, even relative measurements of hemodynamics or metabolic changes face the major problem of a potential contamination from extracerebral tissue layers. Accounting for extracerebral contributions to fNIRS signals is one of the most critical barriers in the field. We present some of the approaches that were proposed to tackle this challenge in the study of cerebral hemodynamics and functional connectivity. Finally, we critically compare fNIRS and functional magnetic resonance imaging by relating their measurements in terms of signal and noise, and by commenting on their complementarity.
关键词： hemodynamics,functional connectivity,functional magnetic resonance imaging,human brain,near-infrared spectroscopy,diffuse optical imaging,non-invasive sensing,functional near-infrared spectroscopy
更新于2025-09-23 15:21:21
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Functionalized Holmium-Doped Hollow Silica Nanospheres for Combined Sonodynamic and Hypoxia-Activated Therapy
摘要： The oxygen concentration dependence of sonodynamic therapy (SDT) and bioreductive therapy can be utilized to design the strategy of synergistic therapy. Herein, holmium-doped hollow silica nanospheres are synthesized and then sequentially modified with chlorin e6, carboxyl poly(ethylene glycol) silane, and prostate stem cell antigen (PSCA) monoclonal antibody. The resultant nanocomposite designated as HHSN-C/P-mAb has good biocompatibility and can specifically target cancer cells overexpressing PSCA. Due to the inner cavity structure and Ho doping, HHSN-C/P-mAb shows high ultrasound (US) imaging contrast capability and excellent high-field magnetic resonance contrast performance. HHSN-C/P-mAb can act as a nanocarrier for loading the bioreductive prodrug tirapazamine (TPZ), and the degradation of the hollow nanospheres under the trigger of acidic microenvironment favors the pH responsive release of TPZ from the material. Upon US irradiation, HHSN-C/P-mAb produces reactive oxygen species to kill the cancer cells, and importantly, the oxygen consumption during SDT induces an intratumoral hypoxic environment to activate the therapeutic function of codelivered TPZ, resulting in a high-effective synergistic therapy. The findings of this study highlight that HHSN-C/P-mAb is a versatile theranostic nanoplatform for efficient cancer treatment.
关键词： magnetic resonance imaging,sonodynamic therapy,ultrasound imaging,holmium-doped hollow silica nanosphere,bioreductive therapy
更新于2025-09-23 15:21:21