- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Reflectance confocal microscopy of cutaneous melanoma. Correlation with dermoscopy and histopathology
摘要： In vivo Confocal Microscopy is a method for non-invasive, real-time visualization of microscopic structures and cellular details of the epidermis and dermis, which has a degree of resolution similar to that obtained with histology. We present a case of cutaneous melanoma in which diagnosis was aided by confocal microscopy examination. We also correlate the observed features with the dermoscopic and histopathological findings. Confocal microscopy proved to be an useful adjunct to dermoscopy, playing an important role as a method 'between clinical evaluation and histopathology'.
关键词： Melanoma,Diagnosis,Dermoscopy,Confocal microscopy
更新于2025-09-23 15:22:29
-
Regression Analysis of Protoporphyrin IX Measurements Obtained During Dermatological Photodynamic Therapy
摘要： Photodynamic therapy (PDT) is a light activated drug therapy that can be used to treat a number of dermatological cancers and precancers. Improvement of efficacy is required to widen its application. Clinical protoporphyrin IX (PpIX) fluorescence data were obtained using a pre-validated, non-invasive imaging system during routine methyl aminolevulinate (MAL)-PDT treatment of 172 patients with licensed dermatological indications (37.2% actinic keratosis, 27.3% superficial basal cell carcinoma and 35.5% Bowen’s disease). Linear and logistic regressions were employed to model any relationships between variables that may have affected PpIX accumulation and/or PpIX photobleaching during irradiation and thus clinical outcome at three months. Patient age was found to be associated with lower PpIX accumulation/photobleaching, however only a reduction in PpIX photobleaching appeared to consistently adversely affect treatment efficacy. Clinical clearance was reduced in lesions located on the limbs, hands and feet with lower PpIX accumulation and subsequent photobleaching adversely affecting the outcome achieved. If air cooling pain relief was employed during light irradiation, PpIX photobleaching was lower and this resulted in an approximate three-fold reduction in the likelihood of achieving clinical clearance. PpIX photobleaching during the first treatment was concluded to be an excellent predictor of clinical outcome across all lesion types.
关键词： photobleaching,aminolevulinic acid (ALA; Ameluz),dermatology,protoporphyrin IX (PpIX),imaging,methyl aminolevulinate (MAL; Metvix),photodynamic therapy (PDT),fluorescence,non-melanoma skin cancer (NMSC)
更新于2025-09-23 15:22:29
-
Autofluorescence and spectral-domain optical coherence tomography of optic disk melanocytoma
摘要： The authors report fundus autofluorescence (FAF) and spectral-domain optical coherence tomography (OCT ) findings of two consecutive patients who presented with optic disk melanocytoma (ODM). A retrospective study was performed by reviewing medical records and ophthalmic imaging examinations. Optical coherence tomography findings were sloped and brightly reflective anterior tumor surface, adjacent retinal desorganization and abrupt posterior optical shadowing. Vitreous seeds were found in one patient. Fundus autofluorescence revealed outstanding hypoautofluorescence at the tumor area and isoautofluorescence at the remaining retina. Optical coherence tomography findings of the reported cases are consistent with those reported in the reviewed literature. Fundus autofluorescence has been used in the assessment of choroidal melanocytic tumors, but not yet in melanocytomas. We assume that this is the first report of these findings and believe that when its pattern has become clearly defined, fundus autofluorescence will be a useful tool to avoid misdiagnosis in suspicious cases and for follow-up.
关键词： Melanoma/diagnosis,Female,Adult,Male,Case reports,Optic nerve neoplasms/diagnosis,Fluorescein angiography,Humans,Tomography, optical coherence
更新于2025-09-23 15:22:29
-
[IEEE 2017 International Conference on Computational Science and Computational Intelligence (CSCI) - Las Vegas, NV, USA (2017.12.14-2017.12.16)] 2017 International Conference on Computational Science and Computational Intelligence (CSCI) - Estimation of Illumination Map from Dermoscopy Images for Extracting Differential Structures Using Gabor Local Mesh Patterns
摘要： Melanoma is the most deadly form of skin cancer and its incidence rate is significantly increasing. The design of an assisted diagnosis system for the detection of melanoma is a challenging task involving various steps related to computer vision. Researchers have concluded that the accurate identification of melanoma requires robust preprocessing steps on dermoscopy images including hair removal, illumination correction etc., that can help in a better detection of melanoma. In this paper, we propose a novel illumination correction algorithm followed by robust feature extraction from dermoscopy images, leading to a better identification of cancer. Illumination correction is based on statistical estimation of illumination content in the images, followed by the extraction of differential structures using a combination of Gabor filtering followed by extracting local mesh patterns, which exhibit physiological significance based on various clinical rules for detecting melanoma. Our experiments show that the proposed technique outperforms all the other methods that have been considered in this paper.
关键词： Texture analysis,Optimization,Pattern recognition,Gabor filters,Melanoma
更新于2025-09-23 15:22:29
-
Switchable Photoacoustic Imaging of Glutathione Using MnO2 Nanotubes for Cancer Diagnosis
摘要： Glutathione is overexpressed in tumor cells and regulates cancer growth, metastasis and drug resistance. Therefore, detecting glutathione levels may greatly facilitate cancer diagnosis and treatment response monitoring. Photoacoustic (PA) imaging is a noninvasive modality for high-sensitivity, high-resolution, deep-tissue optical imaging. Switchable PA probes that offer signal on/off responses to tumor targets would further improve the detection sensitivity and signal-to-noise ratio of PA imaging. Here we explore the use of MnO2 nanotubes as a switchable and biodegradable PA probe for dynamic imaging of glutathione in cancer. Glutathione reduces black MnO2 nanotubes into colorless Mn2+ ions, leading to decreased and “signal off” PA amplitude. In phantoms, we observed a linear response of reduced PA signals of MnO2 nanotubes to increased glutathione concentrations. Using melanoma as the disease model, we demonstrated that MnO2 nanotubes-based PA imaging of glutathione successfully distinguished B16F10 melanoma cells from BEAS-2b normal cells and discriminated B16F10 tumors from healthy skin tissues. Our results showed that MnO2 nanotubes are a potent switchable and biodegradable PA probe for glutathione imaging in cancer diagnosis.
关键词： photoacoustic imaging,nanotubes,cancer,glutathione,melanoma,switchable contrast reagent,MnO2
更新于2025-09-23 15:21:21
-
Improving clinical diagnosis of early-stage cutaneous melanoma based on Raman spectroscopy
摘要： BACKGROUND: Clinical diagnosis of early melanoma (Breslow thickness less than 0.8 mm) is crucial to disease-free survival. However, it is subjective and can be exceedingly difficult, leading to missed melanomas, or unnecessary excision of benign pigmented skin lesions. An objective technique is needed to improve the diagnosis of early melanoma. METHODS: We have developed a method to improve diagnosis of (thin) melanoma, based on Raman spectroscopy. In an ex vivo study in a tertiary referral (pigmented lesions) centre, high-wavenumber Raman spectra were collected from 174 freshly excised melanocytic lesions suspicious for melanoma. Measurements were performed on multiple locations within the lesions. A diagnostic model was developed and validated on an independent data set of 96 lesions. RESULTS: Approximately 60% of the melanomas included in this study were melanomas in situ. The invasive melanomas had an average Breslow thickness of 0.89 mm. The diagnostic model correctly classified all melanomas (including in situ) with a specificity of 43.8%, and showed a potential improvement of the number needed to treat from 6.0 to 2.7, at a sensitivity of 100%. CONCLUSION: This work signifies an important step towards accurate and objective clinical diagnosis of melanoma and in particular melanoma with Breslow thickness <0.8 mm.
关键词： dermatology,Raman spectroscopy,early diagnosis,Breslow thickness,melanoma
更新于2025-09-23 15:21:21
-
Attenuated total reflectance spectroscopy to diagnose skin cancer and to distinguish different metastatic potential of melanoma cell
摘要： Early diagnosing of skin cancer and investigation of metastatic potential of cancer cells are very important to treat it appropriately. Infrared spectroscopy of biological tissues is an emerging technique which gives the spectral differences between healthy and diseased cells. In this work, we have demonstrated that attenuated total re?ectance Fourier transform (ATR-FTIR) spectroscopy can be used in diagnostic of skin cancer and in differentiating metastatic potential of cancer cells. Using IR spectroscopy, we can identify various types of cancer such as basal cell carcinoma, malignant melanoma, nevus and metastatic potential by alternations in hydration level and molecular changes. We examined biopsy of different types of cancer cells to diagnose skin cancer at early stages by using FTIR spectroscopy. To differentiate metastases we examined two human melanoma cells of same patient but at different metastatic potential and two murine melanoma cells with common genetic background but different metastatic potential. Our ?ndings revealed that melanoma changes the permeability of cell membrane and higher metastatic potential is related to the hydration level of cell membrane. Thus, ATR-FTIR spectroscopy is a potential technique to help in early diagnosing of skin cancer and to differentiate different metastatic potentials.
关键词： ATR-FTIR,Metastatic,spectroscopy,melanoma
更新于2025-09-23 15:21:01
-
Introduction to reflectance confocal microscopy and its use in clinical practice
摘要： Re?ectance confocal microscopy (RCM) is a novel technology that provides noninvasive, in vivo imaging of the skin at nearly histologic resolution. In 2016, the US Centers for Medicare and Medicaid Services (CMS) established reimbursement codes for RCM image acquisition and for the reading and interpretation of images. The combination of RCM imaging with dermoscopy has improved the accuracy of skin cancer diagnosis while reducing the number of biopsies of benign skin lesions. With that, we are starting to see more dermatologists and dermatopathologists using RCM in clinical practice. This editorial is to serve as an introduction on RCM imaging with a focus on its usefulness in both the diagnosis and management of skin cancers. We end by brie?y describing the characteristic RCM features of normal skin to serve as a building block for later cases that will explore both the bene?ts and drawbacks of incorporating RCM imaging for benign and malignant lesions.
关键词： innovative technology,noninvasive imaging,skin cancer,melanoma,re?ectance confocal microscopy,lentigo maligna,nonmelanoma skin cancer
更新于2025-09-23 15:21:01
-
Dataset concerning plasmonic thermal destruction of murine melanoma by gold nanoparticles obtained by green chemistry
摘要： The data presented in this article are related to the research paper “Proof of concept of plasmonic thermal destruction of surface cancers by gold nanoparticles obtained by green chemistry” (Ben Hadadda et al, 2019). In this article, we examined plasmonic thermal destruction of murine melanoma using gold nanoparticles obtained by green chemistry. The presented data were obtained by measuring tumor volume and mice weight in different groups of mice murine melanoma B16F10 treated or not with the nanoparticles and coupled to laser irradiation. These data were compared to the clinical reference treatment: anti-PD1 monoclonal antibody. The data were analyzed in order to be able to compare the antitumor effect of our treatment (photothermal plasmonic therapy using gold nanoparticles) and the reference treatment.
关键词： Green chemistry,Photothermal therapy,Cancer treatment,Gold nanoparticles,Murine melanoma
更新于2025-09-23 15:19:57
-
Deep lamellar keratectomy and penetrating scleroplasty using CO <sub/>2</sub> laser photoablation and bimodal grafting for the treatment of a limbal melanoma in a dog
摘要： A novel approach for en bloc resection of a limbal melanoma using a CO2 laser in a dog. Benefits of this advanced technique include excellent surgical precision, improved hemostasis, and the induction of coagulative necrosis of neoplastic cells.
关键词： limbal,canine,melanoma,CO2 laser
更新于2025-09-23 15:19:57